Cir1 (NM_001007799) Rat Tagged ORF Clone

CAT#: RR205911

Cir1 (Myc-DDK-tagged ORF) - Rat CBF1 interacting corepressor (Cir), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cir1
• Synonyms: 1700023b02rik; Cir; RGD1309199
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205911 representing NM_001007799
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGAAGTCATTCGCCAATTTCATGTGTAAAAAGGACTTTCATCCTGCTTCCAAATCCAACATCAAGA
  AGGTATGGATGGCAGAACAGAAAATATCATATGATAAAAAGAAACAAGAAGAATTAATGCAACAGTACCT
  TAAAGAACAAGAGTCATATGATAACAGATTGCTTATGGGAGATGAGCGTGTAAAGAACGGCCTGAATTTC
  ATGTATGAAGCCCCGCCAGGAGTTAAAAAAGAAAACAAAGAGAAAGAAGAAACGGAAGGAGAGACAGAAT
  ACAAGTTCGAATGGCAGAAAGGTGCTCCAAGGGAAAAATATGCTAAAGATGACATGAACATCAGAGATCA
  GCCCTTTGGTATTCAGGTTCGAAATGTAAGGTGTATTAAATGTCACAAGTGGGGCCATGTGAACACAGAT
  CGAGAGTGCCCCTTGTTTGGTCTTTCCGGGATCAATGCAAGTTCAGTTCCTACTGATGGCTCAGGGCCGT
  CGATGCACCCCTCGGAGCTAATAGCGGAGATGAGAAACAGTGGGTTTGCACTGAAACGAAATGTACTGGG
  GAGAAACTTGACCGCAAATGATCCATCGCAGGACTATGTCGCAAGTGACGGTGAAGAAGACCCTGAAGTT
  GAGTTCTTAAAGTCGCTCACCACCAAACAGAAGCAGAAGCTTCTCAGGAAATTGGATAGACTTGAAAAGA
  AAAAAAAGAAAAAGAAAAGTGATAAGAAAAAGAAGAAGTTACAGAAGAGCAAAAATAAACACAAAAAACA
  TAAAAAATCCTCCTCTTCCTCATCTTCTTCCTCCTCCTCCTCCTCTTCTTCCTCCTCCTCTTCCTCTTCC
  TCTTCCTCATCCTCCACCTCCTCCTCGGAGACAGATAGCAGCAGTGAGAGTGACAACAAAGAAAAGAAAG
  AGAAAGAGAAGAGGAAGAAAAGGAAGAAAACCAAGTGTTCTGGAAATAAATCCGGTGACTGTAAAGAGTA
  CAAGTCTAAGAACATGCTTTATGAAGAACTTTCTAGCAGCCATAGTGACAGGGGGAAAGCCCAGGAAAAA
  TTAAGGCTTCTAAAACAAGAGAGTTCTGGAGAAAATAGCACATGGGTCCATTCTGGTTCTGACCGGAAGT
  CCAGAAGCCACCAGCACAGTCCAGAGAGGAAAGGGTCTGACAGAAACGGGGGAAGCCGAAGCCGTAGCCG
  GGCTGAGGGCAGCAGGCGAAGCCGGAGCAGGAGTCCTTGCGGTCAGAAGCACAGGGAAAGGGAGGCGAGG
  AGCCGGCCCCACCAGAGCCCTAGTGAGGAGCAGAAGGGGAGAAAGGGCACCCGAAGCCATGGTGACAGCG
  AGCATAGAAGAGAGCGTGCGCGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205911 representing NM_001007799
  Red=Cloning site Green=Tags(s)
  
  MGKSFANFMCKKDFHPASKSNIKKVWMAEQKISYDKKKQEELMQQYLKEQESYDNRLLMGDERVKNGLNF
  MYEAPPGVKKENKEKEETEGETEYKFEWQKGAPREKYAKDDMNIRDQPFGIQVRNVRCIKCHKWGHVNTD
  RECPLFGLSGINASSVPTDGSGPSMHPSELIAEMRNSGFALKRNVLGRNLTANDPSQDYVASDGEEDPEV
  EFLKSLTTKQKQKLLRKLDRLEKKKKKKKSDKKKKKLQKSKNKHKKHKKSSSSSSSSSSSSSSSSSSSSS
  SSSSSTSSSETDSSSESDNKEKKEKEKRKKRKKTKCSGNKSGDCKEYKSKNMLYEELSSSHSDRGKAQEK
  LRLLKQESSGENSTWVHSGSDRKSRSHQHSPERKGSDRNGGSRSRSRAEGSRRSRSRSPCGQKHREREAR
  SRPHQSPSEEQKGRKGTRSHGDSEHRRERAR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001007799
• ORF Size: 1353 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001007799.1, NP_001007800.1
• RefSeq Size: 1356 bp
• RefSeq ORF: 1356 bp
• Locus ID: 362149
• Cytogenetics: 3q23
• MW: 51.4 kDa