Gpld1 (NM_001100512) Rat Tagged ORF Clone

CAT#: RR206031

Gpld1 (Myc-DDK-tagged ORF) - Rat glycosylphosphatidylinositol specific phospholipase D1 (Gpld1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gpld1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206031 representing NM_001100512
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGTAGGCAGGCTGTGGTCTGGCCTGCTGCTCCTGCTGCTTTTTTTCTGCTCTAGAAGTTCATCTT
  GTGGTCTCTCAACACATGTAGAAATAGGACACAGGGCTCTGCAGTTCCTTCAGCTTCAAGATGGACGCAT
  TAACTACAAAGAGCTGCTCTTAGAACACCAGGATGCATATCAGGCAGGGACGGTGTTTCCTGATGCCTTT
  TACCCTAGCATCTGCAAACAAGGAAAATTCCATGAAGTGTCTGAGAGTACTCACTGGACTCCATTTCTTA
  ATGCCAGCATTCATTATATTCGGGAGAACTACCCTCTGCCCTGGGAGAAGGACACAGAGAAGTTGGTGGC
  TTTCTTGTTTGGAGTCACCTCCCACATGGTCGCTGATGTCAGCTGGCACAGCCTGGGTATTGAACAAGGG
  TTCCTCAGGACAATGGGAGCTATTGATTTTTACGACTCTTATTCTGAGGCTCATTCGGCTGGTGATTTTG
  GAGGAGATGTGTTGAGCCAGTTTGAATTTAATTTTAATTACCTCTCACGGCGCTGGTACGTGCCCATCCA
  AGATCTTCTGAGAATTTATGATAATCTCTACGGTCGGAAAGTCATCACCAAGAATGTCATTGTTGATTGC
  ACATACCTTCAGTTCCTGGAAATGCACGGGGAGATGCTAGCTGTTTCCAAGCTCTATTCCACATACTCTA
  CGAAGTCCCCATTTCTGGTGGAACAGTTCCAAGACTATTTCCTCGGAGGTCTAGATGACATGGCCTTCTG
  GTCCACGAACATTTACCGTTTGACCAGCTTTATGCTGGAGAACGGGACCAGTGACTGCAACCTGCCTGAG
  AACCCTCTGTTCATCTCCTGTGATGGTAGGAAGAACCACACCCTCAGCAGCTCAAAAGTGCAGAAAAATG
  ATTTTCACAGGAATTTGACCATGTTTATAAGTAAAGACATCAGGAAAAACCTCAACTACACAGAAAGAGG
  CGTGTTCTACAGCACAGGCTCCTGGGCCCCGGAATCCGTAACCTTTATGTACCAGACTCTGGAGAGGAAC
  CTGAGGATGATGTTTACTGGCAACTCTCAGACAGCTCTGAAGCATGTCTCCAGTCCCTCTGCCTCCTACA
  CCCTGTCTGTCCCCTATGCCAGGCTCGGCTGGGTCATGGCCTCAGCTGACCTCAACCAGGATGGGCACGG
  CGACCTCGTGGTGGGTGCACCAGGCTACAGCCACCCTGGCCGCTTCCAGATCGGGCGGGTGTACATCATC
  TATGGCAATGATTTGGGCCTGCCCCCCATCGACCTGGACTTAGACAAGGAGGCTCATGGGGTCCTGGAGG
  GCTTCCAGCCTTCAGGTCGCTTTGGCTCAGCTTTGGCAGTGCTGGACTTCAACAAGGATGGGCTGCCTGA
  TCTGGCTGTGGGTGCTCCCTCCGTGGGCTCAGGGCAGCTCACCTACAATGGCTCTGTGTATGTCTACTAT
  GGTTCCCAGCAAGGGAGGTTGTCTTCTTCCCCTAACATCACCATCTCCTGCAAGGACACCTACTGTAATT
  TGGGCTGGGCTCTCTTGGCAGCAGACGCAGATGGAGATGGCCAGCATGATCTGGTGATCAGTTCACCTTT
  TGCACCAGGTGGAGGGAAGCAGAGAGGAATTGTGGCTGCCTTTTATTCACACCCCAGACGGAACGACAAA
  GAGTCACTGACTTTGGACGAAGCTGACTGGAAGGTGAACGGGGAGGAGGACTTCTCATGGTTTGGATACT
  CCCTTCATGGGGTCACAGTGGCAAACAGAAGCCTGCTGCTGATTGGGAGCCCGACCTGGAAGAATATCAG
  CAGGATGGCACGCTCAAGCCACCAAAAGAACCAAGAGAAAAAGAGCCTGGGAAGGGTGTATGGCTACTTC
  CCCCCAAACCGGCAGAGGGAAATCACTATCTCGGGAGATAAGGCAATGGGAAAGCTGGGTACATCCCTGT
  CCAGTGGTTATGTGAGGGTGAATGGCACCCTAACCCAAGTCCTGCTGGTTGGAGCCCCTACTCATGATGA
  CGTGTCTAAAATGGCATTCCTAACCATGACCCTGCACCAGGGTGGAGCCACTCGGATGTACGAGCTAGCC
  CCTGAGAAGACACAGCCTGCTCTGCTCAGCACCTTCAGCGGAGACCGACGTTTTTCTCGATTTGGTAGTG
  TTCTACACCTGACAGACCTGGATGACGATGGCTTAGATGAAATCATCATGGCTGCCCCACTGAGGATAAC
  AGATGTCACTTCTGGACTGCTTGGGGGAGAAGATGGGCGAGTGTACATTTATAATGGCATGCATACCACC
  TTAGGTGATGTGACAGGCAAATGCAAGTCCTGGATGACTCCATGTCCAGAGGAGAAGGCCCAGTATGTGC
  TAATTTCTCCAGAGGCAAGTTCAAGGTTTGGGAGCTCACTGGTGTCTGTGCGGTCTAAGGAAAGGAACCA
  AGTGGTCGTTGCTGCTGGAAGGAGTTCTTGGGGAGCCCGGCTCTCTGGGGCACTTCACGTCTACAGCTTC
  AGCTCAGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206031 representing NM_001100512
  Red=Cloning site Green=Tags(s)
  
  MSVGRLWSGLLLLLLFFCSRSSSCGLSTHVEIGHRALQFLQLQDGRINYKELLLEHQDAYQAGTVFPDAF
  YPSICKQGKFHEVSESTHWTPFLNASIHYIRENYPLPWEKDTEKLVAFLFGVTSHMVADVSWHSLGIEQG
  FLRTMGAIDFYDSYSEAHSAGDFGGDVLSQFEFNFNYLSRRWYVPIQDLLRIYDNLYGRKVITKNVIVDC
  TYLQFLEMHGEMLAVSKLYSTYSTKSPFLVEQFQDYFLGGLDDMAFWSTNIYRLTSFMLENGTSDCNLPE
  NPLFISCDGRKNHTLSSSKVQKNDFHRNLTMFISKDIRKNLNYTERGVFYSTGSWAPESVTFMYQTLERN
  LRMMFTGNSQTALKHVSSPSASYTLSVPYARLGWVMASADLNQDGHGDLVVGAPGYSHPGRFQIGRVYII
  YGNDLGLPPIDLDLDKEAHGVLEGFQPSGRFGSALAVLDFNKDGLPDLAVGAPSVGSGQLTYNGSVYVYY
  GSQQGRLSSSPNITISCKDTYCNLGWALLAADADGDGQHDLVISSPFAPGGGKQRGIVAAFYSHPRRNDK
  ESLTLDEADWKVNGEEDFSWFGYSLHGVTVANRSLLLIGSPTWKNISRMARSSHQKNQEKKSLGRVYGYF
  PPNRQREITISGDKAMGKLGTSLSSGYVRVNGTLTQVLLVGAPTHDDVSKMAFLTMTLHQGGATRMYELA
  PEKTQPALLSTFSGDRRFSRFGSVLHLTDLDDDGLDEIIMAAPLRITDVTSGLLGGEDGRVYIYNGMHTT
  LGDVTGKCKSWMTPCPEEKAQYVLISPEASSRFGSSLVSVRSKERNQVVVAAGRSSWGARLSGALHVYSF
  SSD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001100512
• ORF Size: 2529 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001100512.1, NP_001093982.1
• RefSeq Size: 3212
• RefSeq ORF: 2532
• Locus ID: 291132
• MW: 93.7 kDa
• Gene Summary: an enzyme that is involved in insulin signaling pathways and is regulated by insulin [RGD, Feb 2006]