Lrrfip1 (NM_001014269) Rat Tagged ORF Clone

CAT#: RR206048

Lrrfip1 (Myc-DDK-tagged ORF) - Rat leucine rich repeat (in FLII) interacting protein 1 (Lrrfip1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lrrfip1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206048 representing NM_001014269
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCAGCCCAGAGGGCGCGCAAAACAAGGAAATCGACTGTCTGAGTCCAGAAGCGCAAAGACTGGCGG
  AGGCTAGGCTTGCAGCGAAGCGTGCAGCCCGCGCCGAGGCTCGAGAGATCCGGATGAAGGAGCTGGAACG
  GCAGCAGAAAGAGGTAGAAGAAAGACCGGACAAAGACTTCGCTGAGAAGGGCTCCCGTAACATGCCCAGC
  CTGTCTGCAGCCACACTGGCATCTCTGGGCGGGACTTCCTCCCGGAGAGGGAGTGGAGACACCTCCATCT
  CCATGGACACCGAGGCATCCATCAGGGAGATCAAGGACTCTCTGGCCGAAGTTGAGGAGAAATACAAGAA
  GGCTATGGTCTCCAACGCCCAGCTAGACAACGAGAAGACCAACTTCATGTACCAGGTGGACACACTGAAG
  GACATGCTGCTGGAGCTGGAAGAGCAGCTGGCCGAGTCTCAGAGGCAATACGAGGAGAAGAACAAGGAGT
  TCGAGAGGGAGAAGCATGCCCACAGCATCCTACAGTTCCAGTTTGCCGAAGTGAAAGAGGCCCTGAGGCA
  AAGGGAAGAAATGCTCGAAAAACATGGAATAATCCTAAATTCAGAAATAGCCACCAACGGAGAGACTTCG
  GACACAGTAAATGATGTTGGGTACCAAGCGCCCACGAAGATAACAAAAGAAGAGCTGAATGCCCTCAAGG
  CAGCTGGGGAAGGGACACTAGGAAAAGCCAAAGAAGTGGAGGTGAAAAAGGAAATTGTGGAGAAAGTGGG
  GCAAAGAGAAACCTTACAGGATTCTGAGCAAGAACAGCCCAAACTCAACACAGGAAAGGATTGTGTGGAC
  AGAGGGGTGTTACATCCTGGTGAAAAGGCTGAGAACCAAAGACCCGTTGAAGACAGTGCCCTGTCCCCGG
  GACCACTAGCAGGGGCCAAGTGTGAGCAAGAGGTTCAGAGCCAAGATCAAGAAAACACTTCCATCCTCAA
  AAGCCCAGAGCAGATTGAGTCACACGAGGTCACAAACAAGTCAGACAGTAGGGACAGCAACTCCCCTGAA
  CCATCTAGCTGCCGGGGAGGTCTAGACAGTGAAGTGTCAGGGCCCACTGCCTTGGGTATCAAAAACCAAA
  GTGAAAACTCTATGGATAGTCAGGGTAAAGAAAACCAAGAAGATTTGGGAAAAGGTAGCTTTGAACCACG
  TCCAGACCACGTTTTGGGGCAAACACCTGAAATTGACAAGGTGAGCTGTACAGACTCAAGGGGCACAGGT
  GGGAACCACCTGGAGGATGTGGTACAGGCAGGGGACACCATAGTTGAGGACCAAGTAGGGACAATGGCCT
  CTGCAGAGCAAAGCAAAAGCATGGAGAATCACATTGGAAGAAGTCTGAACGATGGGCTGGGGCAGAGCTC
  AGAGAGAGAACTCGCCCATGAAGCAGCTGAGCTTGAGGAGGCCCTCACTCAGAGTTCACAGGCAGGCGGA
  GAGAACACCGTTACTGAGGCTGAAGATGCAGCAGTAAGAGATGAGAAGCCCCTCCAGGCAGACGTCCAGG
  CAACCCCTGCAGCTCCCACAGTTCAGAGCGGCCATCAGGACACGACAGGGCCAGGTAGCACAGACACCAA
  GCACACATCACCTCACGCAAAAGAACGTAACAAAGCAAAGAGCGAGCAGCAGGCAGAGGCCTTGGATTCA
  CCCCAGAAGAAGACTAAGAACAAGAAGAAGAAGAACAAGAAGAAGAAAGCTGCAGCTCCCATGGAAACCT
  GCAAAGATGCTAACGAGGAGTCAAGCTGTCAGGACCCAGATGTGGGTGATGGGGAGGAAGAAGAGCGGGT
  CCAGGCCACCGACAAGAAATGGGCGGCAGAGACCCCAGAACTGAAAGAAGATCCTCAAAGTCGGCCAAGT
  GGGAAACAGAACGACGCGGAGGAGGACAGCGGTCCAGCTGAAGGTCCCACAGACGTCTTGGATCAGAACA
  GTCTGCAGTGTGCAGACGGGGACATCTCCCCAGTAGGCAGGAAAGGTCCCCAAAGGGATGCCTCTCAGAT
  AGGTGGTGAAGAGGGACTTGTGCCCTCCCAACATCCAGGCCAGGCAGATGAGAAGGGTATTGAGGGCCAC
  AGCGTAGACAACAGTGACCTGTCAGGGGAGCTGGGGGGCTTCAATTCAGAAAGTGGAGAGCAGGCAAGAG
  AGGAGGTTGGGAACAGCAAGAGTAAAGAGGATTGCACCATGTCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206048 representing NM_001014269
  Red=Cloning site Green=Tags(s)
  
  MTSPEGAQNKEIDCLSPEAQRLAEARLAAKRAARAEAREIRMKELERQQKEVEERPDKDFAEKGSRNMPS
  LSAATLASLGGTSSRRGSGDTSISMDTEASIREIKDSLAEVEEKYKKAMVSNAQLDNEKTNFMYQVDTLK
  DMLLELEEQLAESQRQYEEKNKEFEREKHAHSILQFQFAEVKEALRQREEMLEKHGIILNSEIATNGETS
  DTVNDVGYQAPTKITKEELNALKAAGEGTLGKAKEVEVKKEIVEKVGQRETLQDSEQEQPKLNTGKDCVD
  RGVLHPGEKAENQRPVEDSALSPGPLAGAKCEQEVQSQDQENTSILKSPEQIESHEVTNKSDSRDSNSPE
  PSSCRGGLDSEVSGPTALGIKNQSENSMDSQGKENQEDLGKGSFEPRPDHVLGQTPEIDKVSCTDSRGTG
  GNHLEDVVQAGDTIVEDQVGTMASAEQSKSMENHIGRSLNDGLGQSSERELAHEAAELEEALTQSSQAGG
  ENTVTEAEDAAVRDEKPLQADVQATPAAPTVQSGHQDTTGPGSTDTKHTSPHAKERNKAKSEQQAEALDS
  PQKKTKNKKKKNKKKKAAAPMETCKDANEESSCQDPDVGDGEEEERVQATDKKWAAETPELKEDPQSRPS
  GKQNDAEEDSGPAEGPTDVLDQNSLQCADGDISPVGRKGPQRDASQIGGEEGLVPSQHPGQADEKGIEGH
  SVDNSDLSGELGGFNSESGEQAREEVGNSKSKEDCTMS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001014269
• ORF Size: 2214 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001014269.1, NP_001014291.1
• RefSeq Size: 3667 bp
• RefSeq ORF: 2217 bp
• Locus ID: 367314
• Cytogenetics: 9q36
• MW: 80 kDa