Hsp90ab1 (NM_001004082) Rat Tagged ORF Clone

CAT#: RR206060

Hsp90ab1 (Myc-DDK-tagged ORF) - Rat heat shock protein 90kDa alpha (cytosolic), class B member 1 (Hsp90ab1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hsp90ab1
• Synonyms: HSP90-BETA; HSP90B; HSPC2; Hspcb
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206060 representing NM_001004082
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTGAGGAAGTGCACCATGGAGAGGAAGAGGTGGAGACCTTCGCCTTTCAGGCAGAAATTGCCCAGC
  TGATGTCCCTCATCATCAACACTTTCTACTCAAACAAAGAGATTTTCCTCCGGGAGTTGATCTCTAATGC
  TTCTGATGCCCTGGACAAGATTCGGTATGAGAGCCTGACTGACCCTTCCAAGTTGGACAGCGGGAAAGAG
  CTGAAGATTGACATCATCCCCAACCCTCAGGAACGCACGCTGACTTTGGTGGACACAGGCATTGGCATGA
  CCAAGGCTGACCTCATAAATAACTTGGGAACCATTGCTAAGTCTGGTACAAAGGCGTTCATGGAGGCTCT
  CCAGGCTGGTGCAGACATCTCCATGATTGGGCAGTTTGGTGTCGGCTTCTACTCTGCCTACCTGGTGGCA
  GAGAAAGTGGTTGTGATCACGAAGCACAACGATGATGAGCAGTATGCCTGGGAGTCTTCTGCTGGTGGAT
  CCTTCACTGTCCGTGCAGACCACGGTGAGCCCATTGGCCGGGGTACCAAAGTGATCCTTCACCTCAAAGA
  AGACCAGACAGAGTACTTAGAGGAGAGGAGGGTCAAGGAGGTGGTGAAGAAACACTCACAGTTCATAGGC
  TACCCCATCACCCTCTATCTGGAGAAGGAACGCGAGAAGGAGATCAGTGATGATGAGGCAGAGGAAGAGA
  AAGGGGAGAAGGAGGAGGAAGATAAGGAGGATGAGGAGAAGCCTAAGATTGAGGATGTGGGATCTGATGA
  GGAGGATGACAGCGGCAAAGATAAGAAAAAGAAAACAAAGAAGATCAAGGAGAAGTACATTGACCAGGAA
  GAGCTGAATAAGACGAAGCCCATCTGGACCAGAAATCCTGATGACATCACTCAAGAGGAATATGGTGAAT
  TCTACAAGAGCCTCACCAATGACTGGGAAGACCACTTGGCAGTCAAGCACTTCTCTGTAGAAGGGCAGTT
  GGAATTCAGGGCATTGCTCTTCATTCCCCGGCGAGCTCCCTTTGACCTCTTTGAGAACAAGAAGAAGAAG
  AACAACATCAAATTGTATGTCCGTCGTGTGTTCATCATGGACAGCTGTGATGAGCTGATACCTGAGTACC
  TCAACTTCATCCGTGGTGTGGTTGATTCCGAGGACCTGCCTCTGAACATCTCCCGAGAAATGCTCCAGCA
  GAGCAAGATCCTGAAAGTCATCCGCAAAAACATTGTGAAGAAGTGCCTTGAGCTCTTCTCTGAGTTGGCT
  GAAGACAAAGAGAACTACAAGAAATTTTATGAGGCATTCTCTAAGAATTTAAAGCTTGGAATCCATGAGG
  ATTCCACTAACCGTCGCCGCCTCTCTGAGCTCCTTCGCTACCATACCTCTCAGTCTGGAGATGAGATGAC
  TTCCCTGTCAGAGTATGTGTCTCGCATGAAGGAGACACAGAAGTCCATCTACTATATCACTGGTGAGAGC
  AAAGAGCAGGTGGCCAACTCTGCCTTCGTGGAGCGTGTGCGGAAACGGGGCTTTGAGGTGGTATACATGA
  CTGAGCCCATTGATGAGTACTGCGTACAGCAGCTCAAGGAGTTCGATGGCAAGAGCCTGGTCTCCGTGAC
  CAAGGAGGGCCTGGAGCTACCAGAGGATGAGGAAGAGAAGAAAAAAATGGAGGAGAGCAAGGCAAAGTTT
  GAGAATCTCTGCAAGCTCATGAAGGAGATCTTGGACAAGAAGGTTGAAAAAGTGACTATCTCCAATAGGC
  TTGTGTCTTCCCCCTGCTGCATTGTGACCAGCACCTACGGCTGGACAGCCAACATGGAACGGATTATGAA
  GGCCCAGGCACTGCGGGACAACTCGACAATGGGCTACATGATGGCCAAGAAACACCTAGAGATCAACCCT
  GACCACCCCATTGTGGAGACTCTGCGGCAGAAGGCTGAGGCAGACAAAAACGACAAAGCTGTCAAAGACC
  TGGTGGTGCTGCTGTTTGAGACTGCTCTGCTCTCCTCTGGCTTCTCACTTGAGGACCCCCAAACCCACTC
  CAACCGCATCTACCGCATGATTAAACTAGGCCTGGGCATTGATGAAGATGAGGTCACTGCAGAAGAGCCC
  AGTGCTGCTGTTCCCGATGAGATCCCCCCACTGGAGGGTGATGAGGATGCCTCTCGCATGGAAGAAGTGG
  AT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206060 representing NM_001004082
  Red=Cloning site Green=Tags(s)
  
  MPEEVHHGEEEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNASDALDKIRYESLTDPSKLDSGKE
  LKIDIIPNPQERTLTLVDTGIGMTKADLINNLGTIAKSGTKAFMEALQAGADISMIGQFGVGFYSAYLVA
  EKVVVITKHNDDEQYAWESSAGGSFTVRADHGEPIGRGTKVILHLKEDQTEYLEERRVKEVVKKHSQFIG
  YPITLYLEKEREKEISDDEAEEEKGEKEEEDKEDEEKPKIEDVGSDEEDDSGKDKKKKTKKIKEKYIDQE
  ELNKTKPIWTRNPDDITQEEYGEFYKSLTNDWEDHLAVKHFSVEGQLEFRALLFIPRRAPFDLFENKKKK
  NNIKLYVRRVFIMDSCDELIPEYLNFIRGVVDSEDLPLNISREMLQQSKILKVIRKNIVKKCLELFSELA
  EDKENYKKFYEAFSKNLKLGIHEDSTNRRRLSELLRYHTSQSGDEMTSLSEYVSRMKETQKSIYYITGES
  KEQVANSAFVERVRKRGFEVVYMTEPIDEYCVQQLKEFDGKSLVSVTKEGLELPEDEEEKKKMEESKAKF
  ENLCKLMKEILDKKVEKVTISNRLVSSPCCIVTSTYGWTANMERIMKAQALRDNSTMGYMMAKKHLEINP
  DHPIVETLRQKAEADKNDKAVKDLVVLLFETALLSSGFSLEDPQTHSNRIYRMIKLGLGIDEDEVTAEEP
  SAAVPDEIPPLEGDEDASRMEEVD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001004082
• ORF Size: 2172 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004082.1, NM_001004082.2, NM_001004082.3, NP_001004082.3
• RefSeq Size: 2338 bp
• RefSeq ORF: 2175 bp
• Locus ID: 301252
• Cytogenetics: 9q12
• MW: 83.3 kDa
• Gene Summary: heat shock protein; displays chaperone activity; binds to peptides and facilitates their appropriate transport and/or secondary structure [RGD, Feb 2006]