P4ha1 (NM_172062) Rat Tagged ORF Clone

CAT#: RR206083

P4ha1 (Myc-DDK-tagged ORF) - Rat procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide I (P4ha1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: P4ha1
• Synonyms: PHalphaI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206083 representing NM_172062
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCTGGGGTGTATTAATGATGGGGATTCTACTTCCTCAGTGTTCAGCCCATCCAGGCTTTTTTACTT
  CAATTGGTCAGATGACTGACTTGATCCATAATGAGAAAGACCTGGTGACGTCACTAAAAGATTACATTAA
  AGCAGAAGAGGACAAGTTAGAGCAAATCAAAAAATGGGCAGAGAAGTTAGACCGGCTAACAAGTACAGCA
  ACAAAAGATCCAGAAGGGTTTGTCGGACACCCTGTAAATGCATTCAAGTTAATGAAACGTCTGAACACCG
  AGTGGAGTGAGTTGGAGAATCTGATCCTCAAGGATATGTCAGATGGCTTCATCTCTAACCTGACCATTCA
  GAGGCAGTACTTCCCTAACGACGAAGACCAGGTTGGGGCTGCAAAAGCTTTGTTTCGTCTGCAAGACACC
  TACAACCTAGACACGAATACCATCTCGAAGGGCAATCTTCCAGGAGTGAAACACAAGTCTTTTCTAACAG
  CTGAGGACTGCTTTGAGTTGGGCAAAGTGGCCTATACAGAAGCAGATTATTACCACACAGAACTCTGGAT
  GGAGCAGGCTCTGATGCAGCTGGAGGAGGGAGAGATGTCTACTGTAGACAAAGTCTCGGTTCTAGATTAT
  TTGAGCTATGCAGTGTACCAGCAGGGTGACCTGGATAAGGCACTTCTGCTTACAAAGAAACTTCTTGAAC
  TAGATCCTGAACACCAGAGAGCCAATGGTAACTTAGTATATTTTGAGTATATAATGAGTAAAGAAAAAGA
  TGCCAATAAGTCTGCTTCGGGTGACCAGTCTGATCAGAAAACTACACCAAAGAAAAAGGGGATTGCTGTG
  GACTACCTGCCAGAGAGACAGAAGTACGAAATGCTGTGCCGTGGGGAGGGTATCAAAATGACTCCTCGGA
  GACAAAAAAGGCTGTTCTGCCGCTACCATGATGGAAACCGGAATCCTAAATTTATCCTGGCCCCAGCCAA
  GCAGGAGGATGAGTGGGACAAGCCTCGCATCATTCGTTTCCATGACATCATCTCAGATGCCGAGATTGAG
  ATCGTCAAAGATTTAGCAAAGCCCAGGCTGAGCCGAGCTACAGTACATGACCCTGAGACTGGGAAATTGA
  CCACAGCACAGTACAGAGTATCTAAGAGTGCTTGGCTGTCTGGCTATGAAGATCCTGTGGTGTCTCGAAT
  TAATATGAGAATACAAGATCTCACAGGACTGGATGTTTCCACGGCAGAGGAATTACAGGTAGCAAATTAT
  GGAGTTGGAGGACAGTATGAACCCCATTTTGACTTTGCCAGGAAAGACGAGCCGGATGCTTTTAGAGAGC
  TTGGGACAGGAAATAGGATTGCCACGTGGCTCTTCTACATGAGTGATGTGTCTGCTGGAGGCGCTACTGT
  TTTTCCTGAAGTGGGAGCCAGTGTTTGGCCCAAAAAAGGCACTGCTGTCTTCTGGTACAATCTGTTTGCC
  AGTGGAGAAGGAGATTACAGTACACGGCACGCAGCCTGTCCTGTGCTAGTGGGAAACAAATGGGTATCCA
  ACAAATGGCTCCATGAACGTGGACAGGAATTTCGAAGGCCGTGTACCCTGTCAGAATTGGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206083 representing NM_172062
  Red=Cloning site Green=Tags(s)
  
  MIWGVLMMGILLPQCSAHPGFFTSIGQMTDLIHNEKDLVTSLKDYIKAEEDKLEQIKKWAEKLDRLTSTA
  TKDPEGFVGHPVNAFKLMKRLNTEWSELENLILKDMSDGFISNLTIQRQYFPNDEDQVGAAKALFRLQDT
  YNLDTNTISKGNLPGVKHKSFLTAEDCFELGKVAYTEADYYHTELWMEQALMQLEEGEMSTVDKVSVLDY
  LSYAVYQQGDLDKALLLTKKLLELDPEHQRANGNLVYFEYIMSKEKDANKSASGDQSDQKTTPKKKGIAV
  DYLPERQKYEMLCRGEGIKMTPRRQKRLFCRYHDGNRNPKFILAPAKQEDEWDKPRIIRFHDIISDAEIE
  IVKDLAKPRLSRATVHDPETGKLTTAQYRVSKSAWLSGYEDPVVSRINMRIQDLTGLDVSTAEELQVANY
  GVGGQYEPHFDFARKDEPDAFRELGTGNRIATWLFYMSDVSAGGATVFPEVGASVWPKKGTAVFWYNLFA
  SGEGDYSTRHAACPVLVGNKWVSNKWLHERGQEFRRPCTLSELE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172062
• ORF Size: 1602 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_172062.1, NM_172062.2, NP_742059.2
• RefSeq Size: 2145
• RefSeq ORF: 1605
• Locus ID: 64475
• MW: 60.9 kDa
• Gene Summary: catalyzes the hydroxylation of -X-Pro-Gly- triplets to form hydroxyproline [RGD, Feb 2006]