Pdlim2 (NM_001007622) Rat Tagged ORF Clone

CAT#: RR206093

Pdlim2 (Myc-DDK-tagged ORF) - Rat PDZ and LIM domain 2 (Pdlim2), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pdlim2
• Synonyms: MGC94060
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206093 representing NM_001007622
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTTGACTGTGAATGTGGTGGGACCAGCACCTTGGGGCTTCCGCATTAGCGGAGGAAGGGATTTCC
  ATACACCCATCATCGTGACGAAGGTCACAGAGAGGGGCAAGGCGGAGGCAGCTGATCTCCGGCCTGGCGA
  CATCATTGTGGCCATCAATGGAGAGAGTGCCGAGAGCATGCTACATGCGGAGGCCCAAAGCAAGATCCGA
  CAGAGTGCCTCACCCCTAAGACTGCAGCTGGACCGGTCCCAAACTGCCTCTCCTGGGCAGATCAATGGGG
  AGGGCTCCTTGGACATGCTGGCAACTAGATTCCAGGGCTCCCTGAGGACACACCATAACAGCCAGTCTTC
  CCAGAGATCTGCCTGCTTCAGCCCAGCCTCTCTCAGTCCCAGGCCAGACAGCCCTTTCTCCACTCCACCC
  CCTACCAGCCCAATTGCCCTTTCTGGAGAGAATGTGATTGGCTGTAGTTTCCAGAGTCTGACACACTCTC
  CAGGTCTTGCTGCTACTCACCACTTGACCTACCCTGGCCAGCCCACCAGCCAACAGGCCGGCCACAGCAG
  CCCAAGCGACTCCACAGTGAGGGTGCTGCTTCATTCCCCAGGACGACCCTCCAGCCCCAGGTTAAGCAGT
  TTGGACCTGGAGGAGGACTCAGAGGTGTTCAAGATGCTGCAGGAGAACCGCCAGGGACGGGCTGCCCCAA
  GGCAGTCCAGCTCTTTTCGGCTCTTACAGGAAGCCTTGGAGGCTGAGGAAAGAGGCGGCACACCTGCCTT
  TGTGCCCAGCTCGCTGAGTCCCAAGGCTTCCTTGCCCACCTCCAGGGCCTTGGCCACGCCACCCAAACTC
  CACACCTGTGAGAAGTGCAGCGTCAACATCTCGAACCAGGCAGTCCGCATCCAGGAGGGGAGGTACCGAC
  ACCCTGGCTGCTACACCTGTGCAGACTGCGGGCTGAACCTGAAGATGCGGGGTCACTTCTGGGTGGGAAA
  CGAGTTGTACTGTGAGAAGCACGCCCGCCAGCGCTACTCAATGCCTGGAACTCTCAGCTCTCAAGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206093 representing NM_001007622
  Red=Cloning site Green=Tags(s)
  
  MALTVNVVGPAPWGFRISGGRDFHTPIIVTKVTERGKAEAADLRPGDIIVAINGESAESMLHAEAQSKIR
  QSASPLRLQLDRSQTASPGQINGEGSLDMLATRFQGSLRTHHNSQSSQRSACFSPASLSPRPDSPFSTPP
  PTSPIALSGENVIGCSFQSLTHSPGLAATHHLTYPGQPTSQQAGHSSPSDSTVRVLLHSPGRPSSPRLSS
  LDLEEDSEVFKMLQENRQGRAAPRQSSSFRLLQEALEAEERGGTPAFVPSSLSPKASLPTSRALATPPKL
  HTCEKCSVNISNQAVRIQEGRYRHPGCYTCADCGLNLKMRGHFWVGNELYCEKHARQRYSMPGTLSSQA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001007622
• ORF Size: 1047 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001007622.1, NP_001007623.1
• RefSeq Size: 1562 bp
• RefSeq ORF: 1050 bp
• Locus ID: 290354
• Cytogenetics: 15p11
• MW: 37.6 kDa