C1qc (NM_001008524) Rat Tagged ORF Clone

CAT#: RR206122

C1qc (Myc-DDK-tagged ORF) - Rat complement component 1, q subcomponent, C chain (C1qc), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C1qc
• Synonyms: C1qg
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206122 representing NM_001008524
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTGTAGGAACCAGCTGCCAGCCCCAGCATGGACTCTACCTGCTGCTGCTCCTTCTGGCCCTACCCC
  TCAGGAGCCAGGCCAACGCTGGCTGCTATGGGATCCCAGGGATGCCAGGCCTGCCGGGAACCCCTGGGAA
  GGATGGGCATGATGGACTTCAGGGGCCCAAGGGTGAGCCGGGAATCCCAGCCATCCCTGGGACACAAGGA
  CCCAAGGGTCAGAAGGGCGAGCCGGGTATGCCTGGCCATCGTGGGAAAAACGGCCCCATGGGGACCTCTG
  GGTCGCCAGGGGATCCAGGCCCCAGGGGTCCTCCCGGGGAGCCGGGTGAGGAGGGTCGATACAAACAGAA
  GCATCAGTCGGTGTTCACGGTCACCCGGCAGACCGCGCAGTACCCAGCGGCCAATGGCCTTGTCAAGTTC
  AATTCCGCCATCACCAATCCTCAGGGGGATTACAACACAAACACGGGGAAGTTCACCTGCAAAGTGCCCG
  GCCTCTACTACTTCGTCCACCACACATCCCAGACGGCCAACCTGTGCGTGCAGCTGCTCCTCAACAATGC
  CAAGGTGACCAGCTTCTGCGACCACATGTCCAACAGCAAGCAGGTCAGCTCAGGAGGAGTACTCCTGCGG
  CTGCAGAGGGGCGACGAGGTGTGGCTGGCCGTCAACGACTACAACGGCATGGTGGGCACTGAGGGCTCTG
  ACAGCGTCTTCTCTGGTTTCCTACTGTTTCCTGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206122 representing NM_001008524
  Red=Cloning site Green=Tags(s)
  
  MVVGTSCQPQHGLYLLLLLLALPLRSQANAGCYGIPGMPGLPGTPGKDGHDGLQGPKGEPGIPAIPGTQG
  PKGQKGEPGMPGHRGKNGPMGTSGSPGDPGPRGPPGEPGEEGRYKQKHQSVFTVTRQTAQYPAANGLVKF
  NSAITNPQGDYNTNTGKFTCKVPGLYYFVHHTSQTANLCVQLLLNNAKVTSFCDHMSNSKQVSSGGVLLR
  LQRGDEVWLAVNDYNGMVGTEGSDSVFSGFLLFPD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001008524
• ORF Size: 735 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001008524.1, NP_001008524.1
• RefSeq Size: 1060 bp
• RefSeq ORF: 738 bp
• Locus ID: 362634
• Cytogenetics: 5q36
• MW: 25.7 kDa
• Gene Summary: C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes. [UniProtKB/Swiss-Prot Function]