Rasa1 (NM_013135) Rat Tagged ORF Clone

CAT#: RR206131

Rasa1 (Myc-DDK-tagged ORF) - Rat RAS p21 protein activator (GTPase activating protein) 1 (Rasa1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rasa1
• Synonyms: GAPX; Rasa
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206131 representing NM_013135
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGCGGCCGAGGCGGGCAGTGAGGAAGGCGGCCCGGCGACCGCGGGGACCGGCGGCGCCGCGGCCA
  CGGGCTCCAGCGCCTACCCAGCAGCGTGCCGGGTAAAGTTACCCGCGGCCCCTCCGATGGCAGTCGCCCC
  GTGTCCTGGGCTGGCGGACACCGACCTGGCTGCGGCCCTCGGTGGGGGAGCCGCGTCGGGGTCCGGGTTC
  CTGGGGACCGGGCCGGTGTCTGGTGTCCTGGGGGGCGCCGCGCTGACCGGCGGTGCTGCAGCTGGCGTGG
  CGGGTGCTGCAGCCGCTGGACCTGCCGGAGACATCGCTCTCACCAAGGGGACTCTCTCTCTGCCTGCTGA
  GACTCTCGGGCCGGGCGGCGGATTCCCTCCTCTGCCGCCTCCTCCTCTGTTGCCTCCTTTGGGCTCCGGT
  CTGGGGACAGTGGATGAAGGTGACTCCTTGGATGGACCAGAATACGAGGAGGAAGAGGTGGCCATACCGC
  TGACCGCACCTCCAACTAACCAGTGGTATCATGGAAAACTTGATAGAACTATAGCAGAAGAACGCCTCAG
  GCAGGCAGGGAAATCTGGAAGCTACCTTATAAGAGAGAGTGATCGGAGGCCAGGGTCCTTTGTACTTTCA
  TTTCTTAGCCAGACAAATGTTGTCAATCATTTTAGGATTATTGCTATGTGTGGTGATTACTACATTGGTG
  GAAGGCGTTTTTCTTCACTCTCAGACCTAATAGGATATTACAGTCACGTTTCTTGTTTACTTAAAGGAGA
  AAAATTACTCTACCCAGTTGCACCACCAGAGCCAGTAGAAGATAGAAGACGTGTACGAGCCATTCTGCCC
  TACACAAAAGTACCAGACACTGATGAAATAAGTTTCTTAAAGGGAGATATGTTCATTGTTCACAATGAGT
  TAGAAGATGGATGGATGTGGGTTACAAACCTAAGAACAGATGAGCAAGGCCTTATTGTTGAGGACCTAGT
  GGAAGAGGTGGGCCGAGAAGAAGATCCACATGAAGGCAAAATATGGTTCCATGGGAAGATTTCCAAACAA
  GAAGCTTATAATTTATTAATGACAGTTGGCCAAGTCTGCAGCTTTCTTGTGAGGCCCTCAGATAATACTC
  CTGGAGATTATTCCCTGTATTTTCGGACCAATGAAAATATCCAGCGATTTAAAATTTGTCCAACACCTAA
  CAATCAGTTCATGATGGGAGGCCGCTATTATAACAGTATTGGGGACATCATAGACCACTATCGGAAAGAA
  CAGATTGTTGAAGGCTATTATCTTAAGGAACCTGTACCAATGCAGGATCAAGGACAAGTACTCAATGACA
  CTGTGGATGGGAAGGAAATCTATAATACAATTCGTCGTAAAACAAAGGATGCATTTTATAAAAATATTGT
  TAAAAAAGGTTATCTTCTGAAGAAGGGCAAAGGAAAACGTTGGAAAAATTTATATTTTATCTTAGAGGGC
  AGTGATGCCCAACTTATTTATTTTGAAAGTGAAAAACGAGCTACAAAACCAAAAGGGCTAATAGATCTCA
  GTGTATGTTCTGTCTACGTTGTCCATGATAGTCTCTTCGGCAGGCCAAACTGTTTTCAGATAGTCGTTCA
  GCACTTTAGTGAAGAACATTACATCTTTTACTTTGCAGGAGAAACTCCAGAACAAGCAGAGGATTGGATG
  AAAGGCCTGCAAGCATTTTGCAGTTTACGGAAAAGTAGCCCAGGGACATCTAATAAACGCCTTCGTCAGG
  TCAGCAGCCTTGTATTACATATTGAAGAAGCTCATAAACTACCAGTAAAACACTTCACTAATCCATATTG
  TAACATCTACTTGAATAGTGTCCAAGTAGCAAAAACTCATGCAAGGGAGGGGCAAAACCCAGTATGGTCA
  GAGGAGTTTGTCTTTGATGATCTTCCTCCTGACATCAATAGATTTGAAATCACACTTAGTAATAAAACAA
  AGAAAAGTAAAGATCCAGATATCTTATTTATGCGTTGCCAGTTGAGCAGGTTACAGAAAGGACATGCCAC
  AGATGAATGGTTTCTGCTAAGTTCTCATATACCACTAAAAGGTATTGAACCAGGATCTCTGCGTGTTCGA
  GCACGATACTCTATGGAAAAAATCATGCCAGAAGAAGAGTACAGTGAATTTAAAGAGCTTATACTGCAGA
  AGGAACTTCACGTAGTCTATGCTCTATCACACGTATGTGGACAAGACCGAACACTACTGGCCAGCATCCT
  ACTGAAAATATTTCTTCATGAAAAGCTTGAGTCATTGTTGTTATGCACACTAAATGACAGAGAGATAAGT
  ATGGAAGATGAAGCCACTACCCTATTTCGAGCTACAACACTTGCGAGTACCTTGATGGAGCAGTACATGA
  AAGCCACGGCCACGCAGTTTGTTCATCATGCTTTGAAAGACTCCATTTTAAAGATAATGGAAAGCAAGCA
  ATCATGTGAGTTAAGTCCATCAAAGTTAGAAAAAAATGAAGATGTGAACACTAATTTAGCACACCTATTG
  AGCATACTTTCAGAGCTTGTGGAGAAAATATTCATGGCTTCAGAAATACTTCCACCGACACTGAGATATA
  TTTATGGGTGTTTACAGAAATCAGTTCAGCATAAGTGGCCTACAAATAACACCATGAGAACACGAGTTGT
  TAGTGGTTTTGTTTTTCTTCGGCTTATCTGCCCAGCTATCCTGAATCCACGGATGTTTAATATCATCTCA
  GATTCTCCATCTCCTATTGCTGCAAGAACACTGACATTAGTGGCTAAATCTGTGCAGAATTTAGCAAATC
  TTGTGGAATTTGGAGCTAAGGAGCCTTATATGGAAGGTGTCAATCCATTCATCAAAAGTAATAAACATCG
  CATGATCATGTTTTTAGATGAACTTGGGAATGTACCTGAGCTTCCGGACACTACAGAACATTCTAGAACT
  GACCTGTCTCGTGACTTAGCAGCATTGCATGAGATTTGTGTAGCTCATTCAGATGAACTGAGAACACTCA
  GTAATGAGCGTGGTGTACAGCAGCATGTACTGAAAAAGCTTCTGGCAATAACAGAACTGCTTCAACAAAA
  GCAAAACCAGTATACAAAAACCAATGATGTCAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206131 representing NM_013135
  Red=Cloning site Green=Tags(s)
  
  MMAAEAGSEEGGPATAGTGGAAATGSSAYPAACRVKLPAAPPMAVAPCPGLADTDLAAALGGGAASGSGF
  LGTGPVSGVLGGAALTGGAAAGVAGAAAAGPAGDIALTKGTLSLPAETLGPGGGFPPLPPPPLLPPLGSG
  LGTVDEGDSLDGPEYEEEEVAIPLTAPPTNQWYHGKLDRTIAEERLRQAGKSGSYLIRESDRRPGSFVLS
  FLSQTNVVNHFRIIAMCGDYYIGGRRFSSLSDLIGYYSHVSCLLKGEKLLYPVAPPEPVEDRRRVRAILP
  YTKVPDTDEISFLKGDMFIVHNELEDGWMWVTNLRTDEQGLIVEDLVEEVGREEDPHEGKIWFHGKISKQ
  EAYNLLMTVGQVCSFLVRPSDNTPGDYSLYFRTNENIQRFKICPTPNNQFMMGGRYYNSIGDIIDHYRKE
  QIVEGYYLKEPVPMQDQGQVLNDTVDGKEIYNTIRRKTKDAFYKNIVKKGYLLKKGKGKRWKNLYFILEG
  SDAQLIYFESEKRATKPKGLIDLSVCSVYVVHDSLFGRPNCFQIVVQHFSEEHYIFYFAGETPEQAEDWM
  KGLQAFCSLRKSSPGTSNKRLRQVSSLVLHIEEAHKLPVKHFTNPYCNIYLNSVQVAKTHAREGQNPVWS
  EEFVFDDLPPDINRFEITLSNKTKKSKDPDILFMRCQLSRLQKGHATDEWFLLSSHIPLKGIEPGSLRVR
  ARYSMEKIMPEEEYSEFKELILQKELHVVYALSHVCGQDRTLLASILLKIFLHEKLESLLLCTLNDREIS
  MEDEATTLFRATTLASTLMEQYMKATATQFVHHALKDSILKIMESKQSCELSPSKLEKNEDVNTNLAHLL
  SILSELVEKIFMASEILPPTLRYIYGCLQKSVQHKWPTNNTMRTRVVSGFVFLRLICPAILNPRMFNIIS
  DSPSPIAARTLTLVAKSVQNLANLVEFGAKEPYMEGVNPFIKSNKHRMIMFLDELGNVPELPDTTEHSRT
  DLSRDLAALHEICVAHSDELRTLSNERGVQQHVLKKLLAITELLQQKQNQYTKTNDVR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013135
• ORF Size: 3114 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_013135.1, NP_037267.1
• RefSeq Size: 3117
• RefSeq ORF: 3117
• Locus ID: 25676
• MW: 115.4 kDa
• Gene Summary: may play a role in Ras GTPase mediated signal transduction; may be involved in cell proliferation and cell migration [RGD, Feb 2006]