Ascc1 (NM_001007632) Rat Tagged ORF Clone

CAT#: RR206154

Ascc1 (Myc-DDK-tagged ORF) - Rat activating signal cointegrator 1 complex subunit 1 (Ascc1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ascc1
• Synonyms: Asc-1; CGI-18
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206154 representing NM_001007632
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGTTCTGCGCCCACAGCTTGTAACATTCGATGGTCGGAATTATAGAAAGAATCCTGTCCAGGAAA
  AGCAGCCCCAACATGAAGAGGAGGAGGATTTCTATCAAGACTCCATGGAGTACTCGGATGAGCCCTGCGG
  TGCCTATGAGGTGGAGCAGACCCCTCATGGATTCCGGGCCACCGTCAGTGCCCCTAGCCTGCTCTACAAG
  CACATAGTTGGAAAGAGAGGCGATACCAAGAAGAAAATAGAAGTGGAGACTAAAACATCTATTAACATTC
  CTAAACTCGGCCATGAGGGAGAAATCGTGATCACTGGTCATCATCGAAATGGTGTAGTTTCCGCGCGAAC
  TCGGATCGATGTCCTCCTGGACACGTTCAGGAGGAGGCAGCCTTTCACTCACTTCCTCTCCTTCTTCCTC
  AATGAAGTTGAAGTCCAGGAACGGTTTCTGGAGTTCCAGGCGGAAGTACTGAAGAAGTGTTCCAAGGATC
  GTGGGGTTGACAGCACCATTTTTCAGAATCCCAAAAAGCTTCACCTAACTATTGGCATGTTGGTACTCCT
  AAGTGAACAAGAGATCCAGCAGACCTGTGAGATCCTGCAGCGCTGTAAGGAGGAATTCATTAATGACATT
  TCCGGAGGCAAACCCCTGGAAGTAGAGATGGCAGGGATCGAATACATGAATGACGACCCTGCTATGGTGG
  ATGTTCTTTATGCCAAAGTCCACATGAAAGACGGCTCCAACAGGCTGCAGGAACTAGTTGATCGAGTGCT
  GGAACGCTTTCAGTCAATGGGGCTCGTCATGAAGGAGTGGACCAGTGTGAAACTGCACGCCACCGTCATG
  AACACACTCCTCAGGAAAGACCCCAATGCTGAAGGCAGGTACAACCTCTACACAGCAGATGGCAAGTATA
  TCTTCAAGGAAAGAGAATCTTTTGATGGCCGGAACATTTTAAAGACATTTGAGAACTTCTACTTTGGTTC
  CCTGAAGCTGAATTCTATTCACATCTCCCAGAGGTTCACGGTGGACAGCTTCGGAAACTATGCTTCCTGC
  GGACATGTTGACTTCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206154 representing NM_001007632
  Red=Cloning site Green=Tags(s)
  
  MDVLRPQLVTFDGRNYRKNPVQEKQPQHEEEEDFYQDSMEYSDEPCGAYEVEQTPHGFRATVSAPSLLYK
  HIVGKRGDTKKKIEVETKTSINIPKLGHEGEIVITGHHRNGVVSARTRIDVLLDTFRRRQPFTHFLSFFL
  NEVEVQERFLEFQAEVLKKCSKDRGVDSTIFQNPKKLHLTIGMLVLLSEQEIQQTCEILQRCKEEFINDI
  SGGKPLEVEMAGIEYMNDDPAMVDVLYAKVHMKDGSNRLQELVDRVLERFQSMGLVMKEWTSVKLHATVM
  NTLLRKDPNAEGRYNLYTADGKYIFKERESFDGRNILKTFENFYFGSLKLNSIHISQRFTVDSFGNYASC
  GHVDFS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001007632
• ORF Size: 1068 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001007632.1, NM_001007632.2, NP_001007633.1
• RefSeq Size: 1508 bp
• RefSeq ORF: 1071 bp
• Locus ID: 294512
• Cytogenetics: 20q11
• MW: 41.2 kDa