Chek2 (NM_053677) Rat Tagged ORF Clone

CAT#: RR206276

Chek2 (Myc-DDK-tagged ORF) - Rat CHK2 checkpoint homolog (S. pombe) (Chek2), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Chek2
• Synonyms: Chk2; Rad53
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206276 representing NM_053677
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAGTCATCAGTCCCACGGCAGCACCTCCTCGAAGGCTCATGACAGTGCTTCCTGTTCACAGTCTC
  AAGGCGGCTTCAGCCAGTCCCAAGGCACCCCCTCTCAGTTGCACGACCTCTCACAGTATCAGGGTGCATC
  CAGTTCCTCCACCAGCACAGTGCCATCCTCCAGCCAGTCCTCCCACTCCAGCTCTGGGACCCTGAGCTCT
  TTAGAGACAGTGTCTACTCAGGAACTCTGTTCTATTCCTGAGGACCAAGAACCGGAAGAGCCTGGACCTA
  CTCCTTGGGCTCGGCTATGGGCTCTTCAGGATGGATTCTCGAATCTAGACTGTGTTAATGACAACTACTG
  GTTCGGGAGGGATAAAAGCTGTGAATATTGCTTCGATGGACCACTGTTGAAAAGGACGGATAAGTACCGG
  ACTTACAGCAAGAAGCACTTTCGCATCTTCAGGGAAATGGGCCCTAAGAATTGTTACATTGTATACCTAG
  AAGACCACAGTGGAAATGGAACCTTTGTAAATACAGAGCTTATCGGGAAAGGAAAACGCTGTCCTTTGAG
  TAACAACTCTGAAATCGCACTTTCACTGTGTAGAAATAAAGTTTTTGTATTTTTTGATCTGACTGTAGAT
  GACCAGTCAGTTTATCCTAAGGAATTAAGAGACGAATACATCATGTCAAAAACTCTTGGAAGCGGTGCCT
  GTGGGGAGGTAAAGATGGCCTTTGAGAGGAAAACGTGTAAGAAAGTGGCCATAAAGATCATCAGCAAGCG
  GAGGTTCGCCCTTGGCTCGTCCAGAGAAGCTGACACAGCTCCCAGTGTGGAAACTGAAATAGAAATTTTG
  AAGAAGCTGAATCATCCGTGCATCATCAAGATTAAAGATGTCTTTGATGTGGAAGATTATTACATTGTTC
  TGGAACTGATGGAAGGAGGAGAATTGTTTGACCGTGTGGTGGGGAACAAACGCCTGAAGGAAGCCACCTG
  CAAGCTCTATTTCTACCAAATGCTTTTAGCTGTACAGTACCTTCATGAAAATGGGATCATACATCGGGAC
  CTAAAGCCAGAGAATGTTCTTCTGTCATCTCAGGAAGAGGACTGTCTTATCAAGATCACTGATTTTGGAC
  AGTCCAAGATCTTGGGGGAGACCTCCCTGATGAGGACTCTATGTGGTACGCCCACTTATCTGGCTCCTGA
  GGTCCTTATCTCCAACGGGACTGCTGGGTACAGCCGGGCCGTCGACTGCTGGAGCCTAGGAGTCATTCTT
  TTCATCTGCCTAAGTGGGTATCCACCTTTCTCTGAGCATAAGACCCAAGTGTCACTGAAGGATCAGATCA
  CCAGTGGAAAGTACAACCTTATTCCGGAAGTCTGGACAGATGTCTCAGAGAAGGCTCTGGACCTTGTCAA
  GAAACTACTGGTTGTGGATCCAAAGGCCAGGCTTACCACAGAGGAAGCCTTAAGTCATCCTTGGCTTCAG
  GATGAGCACATGAAGAAGAAGTTTCAGGATCTGCTGGTGCAGGAAAAGAACTTGGTGCCCCTCCCTCTGG
  CTCCTGCCCAGACTTCCGGTCAAAAGCGGCCCCTGGAACTGGAGGCGGAGGACGCGGAGAGCTCGAAGCG
  CCTGGCCGTGTGTAAGGCTGTGTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206276 representing NM_053677
  Red=Cloning site Green=Tags(s)
  
  MKSHQSHGSTSSKAHDSASCSQSQGGFSQSQGTPSQLHDLSQYQGASSSSTSTVPSSSQSSHSSSGTLSS
  LETVSTQELCSIPEDQEPEEPGPTPWARLWALQDGFSNLDCVNDNYWFGRDKSCEYCFDGPLLKRTDKYR
  TYSKKHFRIFREMGPKNCYIVYLEDHSGNGTFVNTELIGKGKRCPLSNNSEIALSLCRNKVFVFFDLTVD
  DQSVYPKELRDEYIMSKTLGSGACGEVKMAFERKTCKKVAIKIISKRRFALGSSREADTAPSVETEIEIL
  KKLNHPCIIKIKDVFDVEDYYIVLELMEGGELFDRVVGNKRLKEATCKLYFYQMLLAVQYLHENGIIHRD
  LKPENVLLSSQEEDCLIKITDFGQSKILGETSLMRTLCGTPTYLAPEVLISNGTAGYSRAVDCWSLGVIL
  FICLSGYPPFSEHKTQVSLKDQITSGKYNLIPEVWTDVSEKALDLVKKLLVVDPKARLTTEEALSHPWLQ
  DEHMKKKFQDLLVQEKNLVPLPLAPAQTSGQKRPLELEAEDAESSKRLAVCKAVL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053677
• ORF Size: 1635 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_053677.1, NP_446129.1
• RefSeq Size: 1749
• RefSeq ORF: 1638
• Locus ID: 114212
• MW: 60.9 kDa
• Gene Summary: kinase involved in ATM-dependent DNA damage checkpoint pathway [RGD, Feb 2006]