Bmp10 (NM_001031824) Rat Tagged ORF Clone

CAT#: RR206284

Bmp10 (Myc-DDK-tagged ORF) - Rat bone morphogenetic protein 10 (Bmp10), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Bmp10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206284 representing NM_001031824
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTTCTCTGGTTCTGCCGCTGAGCGCCGTCTTCTGCCTGGTGGCTCGTCTGGCTTCTGGCAGCCCCA
  TCATGGGCCTTGAGCAGTCGCCCCTGGAAGAAGACATGCCCTTCTTTGATGATATCTTCACAGAACAAGA
  TGGTATTGACTTCAACACACTGCTGCAGAGCATGAAGGACGAGTTTCTCAAGACGTTGAATCTGTCAGAC
  ATTCCCCCACAGGACACAGGCAGAGTGGATCCGCCGGAGTACATGCTGGAGCTCTACAACAAATTCGCCA
  CGGACCGGACCTCCATGCCATCTGCTAACATCATCCGGAGCTTCAAGAATGAAGATCTGTTTTCTCAACC
  AGTCAGTTTCAATGGGATCCGGAAGTATCCTCTCCTCTTCAACGTGTCCATCCCTCACCACGAAGAGGTC
  GTCATGGCTGAACTGAGGTTGTACACGCTGGTGCAGAGAGATCGTTTGATGTATGATGGTGTGGACCGTA
  AAATAATCATCTTTGAGGTTCTAGAGAGTGCCGATGGTAGCGAGGATGAGAGGAGCATGCTGGTCTTGGT
  ATCAACGGAGATCTACGGAACCAACAGTGAGTGGGAGACATTTGACATCACGGATGCCACCAGACGTTGG
  CAAAAGTCAGGCCCATCAACCCACCAGCTGGAGATCCACATCGAAAGCAGACAAAACCAAGCTGAGGACA
  CCGGAAGGGGACAACTGGAAATAGATATGAGTGCTCAGAATAAGCACGACCCTTTGCTTGTTGTGTTTTC
  TGATGACCAAAGCGGTGACAAGGAGCAGAAAGAAGAGCTGAATGAACTGATCTCCCACGAGCAGGATCTG
  GACCTGGGCACCGATGGTTTCTTTGGTGGGCCTGATGAAGAGGCTCTCCTGCAGATGAGGTCAAACATGA
  TCGATGACTCTACCGCTCGGATCAGGAGGAACGCCAAGGGGAACTACTGCAAGAAGACTCCACTGTACAT
  CGACTTCAAGGAGATCGGCTGGGACTCCTGGATCATCGCGCCTCCTGGTTACGAGGCCTATGAGTGCCGC
  GGTGTGTGCAACTACCCTCTGGCGGAGCACCTCACACCTACGAAACACGCAATTATCCAGGCCTTGGTCC
  ACCTCAAGAATTCCCAGAAAGCCTCCAAAGCCTGCTGCGTGCCCACGAAGCTGGATCCCATCTCCATCCT
  CTATTTAGATAAAGGTGTTGTCACCTATAAGTTTAAATACGAAGGAATGGCCGTGTCTGAATGTGGCTGT
  AGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206284 representing NM_001031824
  Red=Cloning site Green=Tags(s)
  
  MGSLVLPLSAVFCLVARLASGSPIMGLEQSPLEEDMPFFDDIFTEQDGIDFNTLLQSMKDEFLKTLNLSD
  IPPQDTGRVDPPEYMLELYNKFATDRTSMPSANIIRSFKNEDLFSQPVSFNGIRKYPLLFNVSIPHHEEV
  VMAELRLYTLVQRDRLMYDGVDRKIIIFEVLESADGSEDERSMLVLVSTEIYGTNSEWETFDITDATRRW
  QKSGPSTHQLEIHIESRQNQAEDTGRGQLEIDMSAQNKHDPLLVVFSDDQSGDKEQKEELNELISHEQDL
  DLGTDGFFGGPDEEALLQMRSNMIDDSTARIRRNAKGNYCKKTPLYIDFKEIGWDSWIIAPPGYEAYECR
  GVCNYPLAEHLTPTKHAIIQALVHLKNSQKASKACCVPTKLDPISILYLDKGVVTYKFKYEGMAVSECGC
  R
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001031824
• ORF Size: 1263 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001031824.1, NP_001026994.1
• RefSeq Size: 1266 bp
• RefSeq ORF: 1266 bp
• Locus ID: 500245
• Cytogenetics: 4q34
• MW: 47.8 kDa