Cdc25b (NM_133572) Rat Tagged ORF Clone

CAT#: RR206364

Cdc25b (Myc-DDK-tagged ORF) - Rat cell division cycle 25 homolog B (S. pombe) (Cdc25b), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cdc25b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206364 representing NM_133572
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGTACCCCCGCAGAAGTCTGCGCCGGGCTCAGCTCTCAGTACTGCCCGCGTGCTGGGTGGCATTC
  AGCGGCCGCGCCACCTCTCAGGCTTTGGGTTTGGGTCTGATGGCTTGCTGGGGTCTCCAGAGCGTGCGGC
  TTCCTCCTCTCCGGTTACCACTCTTACCCAGACCATGTACAACCTCGCAGGGCTCGGCAGCGAGACCCCA
  AAAACTCAAGTAGGAAGCCTGTCGTTCCAGAACAGGCTGACAGACCTGTCCCTGTCCAGACGTACCTCTG
  AGTGCTCCCTGTCATCTGAGTCCTCAGAATCTTCTGATGCAGGTCTCTGCATGGACTCCCCTAGCCCTAT
  GGACCCACAGACAGCAGAGCGCACGTTTGAACAGGCCATTCAGGCAGCCAGTCGGGTCATTCAAAAGATG
  CAATTTACCATAAAAGCTTCGGTCTTTGCCAGTGAGGCTGCTGGACATAGTCCTGTGCTACAGAACATCA
  CCAACTCCCAAGCACTGGACAGCTGGGAGAAAGATGAAGCAGGCTACAGAGCTGCCAGTAGTCCTGGGGA
  GGACAAAGAGAACGATGGATATATCTTCAAGATGCCACAGAAGCTCCCTCATTCCAGCTCTGCCCGAGCT
  TTGGCAGAGTGGGCCAGCCGCAGAGAGGCCTTTACCCAGAGGCCCAGCTCAGCCCCTGACTTGATGTGTC
  TTACCACTGACGGGAAGATGGACGTGGAGGAGGCGAGCCCTGTGGCACAGTCTTCCTCCTTGACTCCTGT
  CGAAAGGGCTTGTGAAGAAGATGACGGATTTGTGGACATCCTAGAGAGTGATTTAAAGGATGATGATATG
  GTCCCCGCAGGCATGGAGAACCTCATTAGTGCTCCACTGGTCAAAAAGCTGGATAAGGAAGAGGAACAGG
  ATCTCATCATGTTCAGCAAGTGCCAGCGGCTTTTCCGCTCTCCATCCATGCCGTGCAGTGTGATCCGGCC
  CATCCTCAAGAGGCTAGAGCGGCCCCACGACAGGGACGTGCCTGTCCTGAGCAAGCGCAGGAAGAGTGGG
  ACACCCCTGGAAGAGCAGCAGCTTGAAGAACCCAAGGCCCGGGTCTTCCGCTCAAAGTCCCTGTGTCACG
  AGATTGAGAGCATCCTGGACAGTGACCACCGTGGCCTGATTGGAGATTACTCTAAGGCCTTCCTCCTGCA
  GACTGTGGATGGCAAACACCAAGACCTCAAGTACATCTCACCAGAAACTATGGTGGCCCTGTTGACAGGC
  AAGTTCAGCAACATCGTGGAGAAGTTTGTAATTGTGGACTGCAGATACCCCTATGAGTATGAAGGCGGGC
  ATATCAAGAATGCTGTGAACTTGCCCCTGGAACCGGATGCCGAGACCTTTCTGCTGAAGCATCCCATCAC
  GCCTTGTAACCTGGACAAGAGAATCATCCTCATTTTCCACTGTGAATTCTCATCTGAGCGTGGACCACGG
  ATGTGCCGCTTCATCAGGGAACGGGACCGTGCAGCTAACGACTATCCCAGCCTGTACTACCCTGAGATGT
  ACATCCTCAAAGGCGGCTACAAGGAGTTCTTCCCGCAGCATCCGAACTTTTGTGAGCCCCAGGACTACCG
  ACCCATGAACCATGCAGCTTTCCGGGATGAGCTGAGGAACTTCCGCCTCAAGACACGCAGCTGGGCTGGG
  GAACGGAGTACCACCCAACTCTGTAGCAGGCTGCAAGACCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206364 representing NM_133572
  Red=Cloning site Green=Tags(s)
  
  MEVPPQKSAPGSALSTARVLGGIQRPRHLSGFGFGSDGLLGSPERAASSSPVTTLTQTMYNLAGLGSETP
  KTQVGSLSFQNRLTDLSLSRRTSECSLSSESSESSDAGLCMDSPSPMDPQTAERTFEQAIQAASRVIQKM
  QFTIKASVFASEAAGHSPVLQNITNSQALDSWEKDEAGYRAASSPGEDKENDGYIFKMPQKLPHSSSARA
  LAEWASRREAFTQRPSSAPDLMCLTTDGKMDVEEASPVAQSSSLTPVERACEEDDGFVDILESDLKDDDM
  VPAGMENLISAPLVKKLDKEEEQDLIMFSKCQRLFRSPSMPCSVIRPILKRLERPHDRDVPVLSKRRKSG
  TPLEEQQLEEPKARVFRSKSLCHEIESILDSDHRGLIGDYSKAFLLQTVDGKHQDLKYISPETMVALLTG
  KFSNIVEKFVIVDCRYPYEYEGGHIKNAVNLPLEPDAETFLLKHPITPCNLDKRIILIFHCEFSSERGPR
  MCRFIRERDRAANDYPSLYYPEMYILKGGYKEFFPQHPNFCEPQDYRPMNHAAFRDELRNFRLKTRSWAG
  ERSTTQLCSRLQDQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133572
• ORF Size: 1722 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_133572.1, NP_598256.1
• RefSeq Size: 2804
• RefSeq ORF: 1725
• Locus ID: 171103
• MW: 64.3 kDa
• Gene Summary: phosphatase; involved in the start of the cell cycle [RGD, Feb 2006]