Phgdh (NM_031620) Rat Tagged ORF Clone

CAT#: RR206378

Phgdh (Myc-DDK-tagged ORF) - Rat phosphoglycerate dehydrogenase (Phgdh), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Phgdh
• Synonyms: MGC105399
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206378 representing NM_031620
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTTCGCAAATCTGCGCAAAATACTCATCAGTGATAGCCTCGACCCCTGCTGCCGGAAGATCCTGC
  AAGATGGAGGGCTGCAGGTGGTGGAGAAGCAGAACTTGAGCAAGGAGGAGCTGATAGCCGAACTCCAGGA
  CTGTGAAGGCCTTATCGTCCGGTCAGCTACTAAGGTCACTGCTGATGTCATCAATGCAGCAGAGAAGCTC
  CAGGTGGTGGGCAGGGCTGGTACAGGCGTGGACAATGTGGATCTGGAGGCTGCCACAAGGAAGGGCGTCC
  TCGTCATGAACACCCCCAATGGAAATAGCCTCAGTGCTGCGGAACTCACCTGTGGGATGCTCATGTGCCT
  GGCCAGGCAGATCCCCCAGGCGACGGCTTCGATGAAAGATGGCAAATGGGACCGGAAGAAGTTCATGGGG
  ACAGAGCTGAACGGGAAGACACTGGGAATTCTTGGCCTGGGCAGAATTGGAAGAGAGGTGGCCGCCCGAA
  TGCAGGCCTTTGGAATGAAGACTGTAGGCTATGACCCCATCATTTCTCCAGAAGTCGCTGCCTCCTTTGG
  TGTTCAGCAGCTGCCGCTAGAGGAGATCTGGCCTCTCTGTGATTTCATCACTGTCCATACCCCGCTCCTG
  CCCTCCACTACAGGCTTGCTCAATGACAGCACCTTTGCCCAGTGCAAGAAAGGCGTGCGGGTGGTGAACT
  GTGCTCGAGGAGGCATTGTGGATGAAGGTGCCCTGCTCCGTGCCCTGCAGTCTGGTCAGTGTGCTGGTGC
  TGCACTGGATGTGTTTACAGAAGAGCCACCACGGGACCGGGCCTTAGTGGACCACGAGAACGTCATCAGC
  TGTCCCCACCTGGGCGCCAGCACCAAGGAGGCCCAGAGCCGCTGTGGGGAGGAAATCGCAGTCCAGTTTG
  TGGACATGGTGAAGGGGAAATCTCTAACAGGGGTTGTAAACGCCCAGGCTCTTACCAGTGCCTTCTCTCC
  ACACACCAAGCCTTGGATTGGTCTGGCAGAAGCATTGGGCACACTGATGCACGCCTGGGCTGGCTCCCCT
  AAAGGGACCATCCAGGTGGTGACACAAGGAACATCTCTGAAGAATGCTGGGACCTGCCTGAGCCCTGCGG
  TCATTGTCGGCCTTCTGAGAGAAGCATCAAAACAGGCAGATGTGAACTTGGTGAACGCTAAGCTACTGGT
  GAAAGAGGCTGGCCTCAATGTCACCACCTCCCACAGTCCTGGTGTCCCAGGAGAGCAGGGCATCGGGGAA
  TGCCTCCTGACTGTGGCCTTGGCAGGTGCTCCCTACCAAGCTGTGGGCTTGGTCCAGGGCACCACACCAA
  TGTTGCAGATGCTCAACGGAGCTGTCTTCAGGCCAGAGGTGCCTCTACGCAGGGGCCAGCCCCTGCTCCT
  GTTCCGGGCTCAGCCCTCCGACCCTGTCATGCTGCCCACTATGATCGGCCTACTGGCAGAGGCGGGGGTA
  CAGCTGCTGTCCTACCAGACCTCCAAGGTGTCTGACGGAGACACTTGGCACGTCATGGGCCTCTCCTCCC
  TACTGCCCAGCCTGGACGCATGGAAGCAGCATGTTTCTGAGGCTTTCCAGTTCTGCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206378 representing NM_031620
  Red=Cloning site Green=Tags(s)
  
  MAFANLRKILISDSLDPCCRKILQDGGLQVVEKQNLSKEELIAELQDCEGLIVRSATKVTADVINAAEKL
  QVVGRAGTGVDNVDLEAATRKGVLVMNTPNGNSLSAAELTCGMLMCLARQIPQATASMKDGKWDRKKFMG
  TELNGKTLGILGLGRIGREVAARMQAFGMKTVGYDPIISPEVAASFGVQQLPLEEIWPLCDFITVHTPLL
  PSTTGLLNDSTFAQCKKGVRVVNCARGGIVDEGALLRALQSGQCAGAALDVFTEEPPRDRALVDHENVIS
  CPHLGASTKEAQSRCGEEIAVQFVDMVKGKSLTGVVNAQALTSAFSPHTKPWIGLAEALGTLMHAWAGSP
  KGTIQVVTQGTSLKNAGTCLSPAVIVGLLREASKQADVNLVNAKLLVKEAGLNVTTSHSPGVPGEQGIGE
  CLLTVALAGAPYQAVGLVQGTTPMLQMLNGAVFRPEVPLRRGQPLLLFRAQPSDPVMLPTMIGLLAEAGV
  QLLSYQTSKVSDGDTWHVMGLSSLLPSLDAWKQHVSEAFQFCF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031620
• ORF Size: 1599 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_031620.1, NP_113808.1
• RefSeq Size: 1803
• RefSeq ORF: 1602
• Locus ID: 58835
• MW: 56.5 kDa
• Gene Summary: catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate and did not catalyse the reduction of 2-oxoglutarate [RGD, Feb 2006]