Crhr1 (NM_030999) Rat Tagged ORF Clone

CAT#: RR206382

Crhr1 (Myc-DDK-tagged ORF) - Rat corticotropin releasing hormone receptor 1 (Crhr1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Crhr1
• Synonyms: CRFR1; CRH-R 1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206382 representing NM_030999
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGACGGCGCCCGCAGCTCCGGCTCGTGAAGGCCCTTCTCCTTCTGGGGCTGAACCCTGTGTCCACCT
  CCCTTCAGGATCAGCGCTGTGAGAACCTGTCCCTGACCAGCAATGTTTCTGGCCTGCAGTGCAATGCATC
  CGTGGACCTCATTGGCACCTGCTGGCCCCGGAGCCCTGCGGGCCAGTTGGTGGTCCGACCCTGCCCTGCC
  TTTTTCTACGGTGTCCGCTACAACACGACAAACAATGGCTACCGGGAGTGCCTGGCCAACGGCAGCTGGG
  CAGCCCGTGTGAATTATTCTGAGTGCCAGGAGATTCTCAACGAAGAGAAGAAGAGCAAAGTACACTACCA
  TGTTGCAGTCATCATCAACTACCTGGGTCACTGCATCTCCCTGGTAGCCCTCCTGGTGGCCTTTGTCCTC
  TTCTTGCGGCTCAGGAGCATCCGGTGCCTGAGAAACATCATCCACTGGAACCTCATCTCGGCTTTCATCC
  TACGCAACGCCACGTGGTTTGTGGTCCAGCTCACCGTGAGCCCCGAGGTGCACCAGAGCAATGTGGCCTG
  GTGTAGGTTGGTGACAGCCGCCTACAATTACTTCCATGTAACCAACTTCTTCTGGATGTTCGGTGAGGGC
  TGCTACCTGCACACAGCCATTGTGCTCACGTACTCCACCGACCGTCTGCGCAAGTGGATGTTCGTCTGCA
  TTGGCTGGGGTGTACCTTTCCCCATCATTGTGGCTTGGGCCATTGGGAAGCTGCACTACGACAATGAAAA
  GTGCTGGTTTGGCAAACGTCCTGGGGTATACACTGACTACATCTACCAGGGCCCCATGATCCTGGTCCTG
  CTGATCAACTTTATCTTTCTCTTCAACATTGTCCGCATCCTCATGACCAAACTCCGGGCATCCACTACAT
  CTGAGACCATTCAGTACAGGAAGGCTGTGAAGGCCACTCTGGTGCTCCTGCCCCTTCTGGGCATCACCTA
  CATGTTGTTCTTCGTCAACCCTGGAGAGGACGAGGTCTCCAGGGTCGTCTTCATCTACTTCAACTCTTTT
  CTGGAGTCCTTTCAGGGCTTCTTTGTGTCTGTGTTCTACTGTTTTCTGAACAGTGAGGTCCGCTCCGCTA
  TCCGGAAGAGGTGGCGTCGGTGGCAGGACAAGCACTCCATCAGAGCCCGAGTGGCCCGAGCTATGTCCAT
  CCCCACCTCCCCGACCAGAGTCAGCTTTCACAGCATCAAGCAGTCCACAGCAGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206382 representing NM_030999
  Red=Cloning site Green=Tags(s)
  
  MGRRPQLRLVKALLLLGLNPVSTSLQDQRCENLSLTSNVSGLQCNASVDLIGTCWPRSPAGQLVVRPCPA
  FFYGVRYNTTNNGYRECLANGSWAARVNYSECQEILNEEKKSKVHYHVAVIINYLGHCISLVALLVAFVL
  FLRLRSIRCLRNIIHWNLISAFILRNATWFVVQLTVSPEVHQSNVAWCRLVTAAYNYFHVTNFFWMFGEG
  CYLHTAIVLTYSTDRLRKWMFVCIGWGVPFPIIVAWAIGKLHYDNEKCWFGKRPGVYTDYIYQGPMILVL
  LINFIFLFNIVRILMTKLRASTTSETIQYRKAVKATLVLLPLLGITYMLFFVNPGEDEVSRVVFIYFNSF
  LESFQGFFVSVFYCFLNSEVRSAIRKRWRRWQDKHSIRARVARAMSIPTSPTRVSFHSIKQSTAV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030999
• ORF Size: 1245 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_030999.1, NM_030999.2, NM_030999.3, NM_030999.4, NP_112261.1
• RefSeq Size: 1248
• RefSeq ORF: 1248
• Locus ID: 58959
• MW: 48.3 kDa
• Gene Summary: This gene encodes a member of the G-protein coupled receptor family. The encoded protein is a receptor for corticotropin-releasing factor (CRH) and urocortin (UCN). The interaction of this protein with CRH and UCN triggers G-protein coupled signaling. This protein plays a pivotal role in mediating the CRH-elicited effects in depression and anxiety. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2014]