Gcs1 (NM_031749) Rat Tagged ORF Clone

CAT#: RR206473

Gcs1 (Myc-DDK-tagged ORF) - Rat glucosidase 1 (Gcs1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gcs1
• Synonyms: Mogs
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206473 representing NM_031749
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCGGGGCGAGAGGCGACGGCGCGCTGCGGCCGCAGAGGGGGCGCGCCCGCTGGAGAGGGCGCGGG
  GTGCGGGCCGGCGGGATGGCAGAGCCGGCGGGGCGCGCGGCTCGGCGGGCGGCGCGGCCCTGGCCGTGGT
  GGTTTTGGCGCTGGCCTTCGGCCTGTCGGGGCGCTGGGTGCTGGCGTGGCTGGGTGTGCGCCGCGCGCTC
  ACACTGCACCCCGCTCCGTCAGCGCTACCGCCGGACTCCTCCAGTCCTGCCGTGGCCCCGGAATTTTTTT
  GGGGTACCTATCGCCCACACGTCTATTTCGGCATGAAGACTCGCAGCCCCAAACCACTGCTCACGGGACT
  GATGTGGGCGCAGCAAGGCGCCACCCCGGGGACCCCTCCTAAGCTCAGGCACACATGCGAACAAGGAGAC
  GGCGTGGGTCCCTATGGCTGGGAGTTCCATGATGGACTCTCCTTTGGTCGGCAACATATCTATGATGGGG
  CCTTAAGGCTCACCACTGAGTTCGTCAAGAGATCTGGGGGTCATCACGGAGGGGATTGGAGCTGGAGAGT
  TACCGTAGAGCCTCAGGCCTCAGGTACACCTTCCTTCCCTTTGGTCTCCCTGTTCTTCTACGTGGTAACA
  GATGGGCAAGAGGTCCTACTACCAGAGGTTGGAGCCAAAGGACAGTTGAAGTTCATCAGTGGGCACACCA
  GTGAACTTGGTGACTTCCGCCTCACACTTCTGCCACCAACCACTCCAGGAGACACTGTCCCTAAGCATGG
  CAGCTACAATGTCTTCTGGTCCTCCAACCCAGGGCTGCCTCTGCTCACAGACATGGTAAAGAGCCACCTA
  AACAGCTGGTTTCATCACCGGCCCCCAGGAGCCTCTCCGGAACGATACCTTGGCTTGCCTGGATCTCTTA
  AGTGGGAAGAGAGAGGCCCTAGTGGGCAAGGGCAGTTCTTGGTCCAGCAGGTGACACTGAAAGCCCCTTT
  CTCTGTGGAGTTTGTGTTTGAATCTGGCAGTGCCAGGACAGGAAGAGACCAAGCCTCTGAGCAGCTGGTA
  GGTGGCCAACTGACCCGGGCCCTGGAGAGCCATGCTGCAGCATTCAAGGAGCGTTTTGAGAGGACCTTCC
  AGCTAAAGGAGAAGGGCCTGAGCCCTGAGGAGCAGGCTCTGGGTCAGGTTGCCCTCAGTGGCCTACTTGG
  TGGGATTGGTTACTTCTATGGACAGGGTTTGGTGTTGCCAGACACTGGCATGGAAGGGTCTGAACAGAAA
  ATGGACCCTTCCCTATTTCCGCCTGTCCCCCTTTTCTCTGGGGTCCCTTCCCGGTCATTCTTCCCACGAG
  GCTTCCTCTGGGATGAAGGCTTTCACCAGCTAGTGGTCCAGCGATGGGATCCCCACCTCACCCGGGAGGC
  CCTAGGACACTGGCTGGGGCTGCTCAATGCTGACGGTTGGATTGGGCGAGAGCAGATTCTGGGGGATGAG
  GCCAGAGCCCGAGTGCCCCCGGAATTCCTAGTGCAACGTGCAGCCCATGCCAACCCTCCAACCCTGCTCC
  TGCCAGTCATACACATGCTAGAAGGCCGGGCCCCTGAGGACTTGGCCTTCCTGCGCAGGGCTTTCCCCCG
  CTTGCATGCCTGGTTCTCTTGGCTCCATCAAAGTCAGGCAGGGCCAGTGCCACTTTCCTACCGCTGGCGG
  GGCAGGGATCTAGCTTTGCCTACCCTACTTAACCCCAAGACACTGCCCTCGGGCCTGGATGACTACCCCA
  GGGCCTCACATCCTTCTGCAGCAGAGCGGCACCTGGACCTGCGGTGCTGGGTGACATTGGGTGCCCGGGT
  GCTGTCACAGCTTGCAGAAGAGCTTGGGGAGACTGAAGCTGCTGCAGAGTTGGGCCCATTGGCTGCCTCT
  CTAGAAGCAGCTGGAAGTCTCGATGAGCTGCACTGGGCTCCTGAGCTGGGTGTCTTTGCCGACTTTGGGA
  ACCACACAAAAGCAGTACAGCTCAAGTCTAGGCCCCCACAGGGGCTGGTTAGGGTGGTAGGCAGGCCTCC
  AGCTCGACTGCAGTATGTGGATGCCCTGGGCTATGTCTCTCTTTTTCCTTTGCTGCTACAACTGCTGGAG
  CCCAGCTCACCCCGCTTGGGACCCCTGCTGGACGTTCTAGCTGATAGCCGCCATCTCTGGAGCCCCTTTG
  GGTTGCGCTCCCTTTCAGCCTCCAGTCTCTTTTATAAACAGCGAAATACGGAACACGATCCACCTTACTG
  GCGAGGTGCGGTGTGGCTGAATATCAACTACCTGGCTTTGGGGGCACTACACCACTATGGGCGTGTGGAG
  GGTCCTCACAAGGTTCAGGCTGCCAAACTCTACCGTGAACTTCGGGCCAATGTAGTAAGCAACGTACGGC
  AGCAATACCAGGCTACAGGGTTTCTGTGGGAACAGTACAGTGACCAGGATGGGCGGGGCATGGGCTGCCG
  CCCCTTCCAAGGATGGACCAGTCTTGTTTTACTGATCATGGCTGAAGAGTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206473 representing NM_031749
  Red=Cloning site Green=Tags(s)
  
  MARGERRRRAAAAEGARPLERARGAGRRDGRAGGARGSAGGAALAVVVLALAFGLSGRWVLAWLGVRRAL
  TLHPAPSALPPDSSSPAVAPEFFWGTYRPHVYFGMKTRSPKPLLTGLMWAQQGATPGTPPKLRHTCEQGD
  GVGPYGWEFHDGLSFGRQHIYDGALRLTTEFVKRSGGHHGGDWSWRVTVEPQASGTPSFPLVSLFFYVVT
  DGQEVLLPEVGAKGQLKFISGHTSELGDFRLTLLPPTTPGDTVPKHGSYNVFWSSNPGLPLLTDMVKSHL
  NSWFHHRPPGASPERYLGLPGSLKWEERGPSGQGQFLVQQVTLKAPFSVEFVFESGSARTGRDQASEQLV
  GGQLTRALESHAAAFKERFERTFQLKEKGLSPEEQALGQVALSGLLGGIGYFYGQGLVLPDTGMEGSEQK
  MDPSLFPPVPLFSGVPSRSFFPRGFLWDEGFHQLVVQRWDPHLTREALGHWLGLLNADGWIGREQILGDE
  ARARVPPEFLVQRAAHANPPTLLLPVIHMLEGRAPEDLAFLRRAFPRLHAWFSWLHQSQAGPVPLSYRWR
  GRDLALPTLLNPKTLPSGLDDYPRASHPSAAERHLDLRCWVTLGARVLSQLAEELGETEAAAELGPLAAS
  LEAAGSLDELHWAPELGVFADFGNHTKAVQLKSRPPQGLVRVVGRPPARLQYVDALGYVSLFPLLLQLLE
  PSSPRLGPLLDVLADSRHLWSPFGLRSLSASSLFYKQRNTEHDPPYWRGAVWLNINYLALGALHHYGRVE
  GPHKVQAAKLYRELRANVVSNVRQQYQATGFLWEQYSDQDGRGMGCRPFQGWTSLVLLIMAEEY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031749
• ORF Size: 2502 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_031749.1, NM_031749.2, NP_113937.1
• RefSeq Size: 2702
• RefSeq ORF: 2505
• Locus ID: 78947
• MW: 91.9 kDa
• Gene Summary: human homolog cleaves the distal alpha-1,2-linked glucose residue from Glc(3)-Man(9)-GlcNAc(2); involved in the N-linked oligosaccharide processing pathway [RGD, Feb 2006]