Repin1 (NM_001005893) Rat Tagged ORF Clone

CAT#: RR206496

Repin1 (Myc-DDK-tagged ORF) - Rat replication initiator 1 (Repin1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Repin1
• Synonyms: Ap-4; Ap4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206496 representing NM_001005893
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGAACGGCGCTGCAGGGGCCCCACGGCCATGGGCCCAGCCCATCCCTGGCTTTTTTCTGGGCCCT
  CCCAGGAGTCCTCCCAGCCCAACAGAGGGTTGAGGTATCAAGGCAAATCAGTAGCACAGCCAGGAGGCCC
  AGCCCCAGTCAAGGTCCATCGTTGTGCCCACTGTCGGAAGCGTTTCCCAGGCTGGGTGGCCCTGTGGCTT
  CACACTCGGCGGTGCCAGGCCCGGCTGCCTCTGCCCTGCCATGAGTGCAACCAGCGCTTTCGCCACGCCC
  CCTTCTTAGCGCTGCATCTTCAGGTTCATGCTTCTGCAGTCCCTGACCTGGGTTTCATCTGCCACCTATG
  TGGGCATAGCTTCCGAGGCTGGGTAGCCCTGGTTCTGCATCTGCGGGCTCACTCGGCTTCAAAGAGGCCC
  ATCACTTGTCCTGAATGCAACAAACGCTTCTGGCGACAAAAACAGCTTCGAGCTCACCTGCGGAGGTGCC
  AGCCCCCTGCCCCTGAGGCCCGGCCCTTCATATGTGGCAACTGTGGCCGGAGCTTCGCCCAATGGGACCA
  GCTCGTTGTTCACAAGAGGGTGCACGTGGCTGAGGCCTTGGAGGAGGCAGCAGCCAAGGCCCTGGGTCCT
  CGCCCGCGAGGACGTCCTTCAGTGACTGCTCCCAGGCCTGGTGGAGATGCTGTGGACCGCCCCTTCCAGT
  GTGCCTGCTGCGGCAAGCGCTTCCGCCACAAGCCCAACCTGATTGCCCACCGCCGCGTGCACACTGGCGA
  GCGACCACACCAGTGCCCAGAGTGCGGGAAGCGTTTCACCAACAAGCCCTACCTGACCTCGCACCGCCGT
  ATACATACGGGCGAGAAGCCCTACCCATGCACCGAGTGTGGCCGCCGCTTCCGCCACAAACCCAACCTGT
  TGTCGCACAGTAAAATCCACAAGCGCTCAGAGGTCTCAGCGCAGGCTGCCTCCCACACCGGGAGTCACTT
  GATTGCAGCGGAGCCTATGGCACAACCTGCGCTTGGGGTGCCCCTGGGGTCCCTGAGGACCCCGGCCGAG
  GCACCTGCGTCCCTGCATAGCTGCACTGACTGCGGCCGCAGCTTCCGGCTGGAGCGTTTCCTGCGGCTAC
  ACCAGCGGCAGCACACAGGCGAGAGGCCCTTCACCTGCACAGAGTGTGGCAAGAACTTCGGCAAGAAAAC
  GCACCTGGTGGCGCACTCACGCGTGCACTCGGGCGAGCGGCCCTTTGCCTGCGAGGAGTGTGGTCGCCGT
  TTCTCACAGGGCAGCCACCTGGCAGCCCACCGGCGCGACCATGCACCAGAGAGACCCTTTGTGTGCCCAG
  ACTGCGGCAAGGCTTTCCGCCACAAGCCCTACCTGGCCGCGCACCGACGCATCCACACAGGCGAGAAGCC
  CTATGTGTGTCCCGACTGTGGCAAAGCTTTCAGCCAGAAGTCCAACCTGGTGTCCCACCGGCGCATCCAC
  ACAGGTGAGCGGCCCTACGCCTGCCCCGACTGTGATCGCAGCTTCAGTCAGAAGTCCAACCTTATCACAC
  ACCGGAAGAGCCACATCCGGGATGGCGCCTTCTGCTGTGCCATCTGCGGCCAGACCTTTGACGATGAGGA
  CCGTCTCTTGATGCACCAGAAGAAGCACGATGCC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206496 representing NM_001005893
  Red=Cloning site Green=Tags(s)
  
  MLERRCRGPTAMGPAHPWLFSGPSQESSQPNRGLRYQGKSVAQPGGPAPVKVHRCAHCRKRFPGWVALWL
  HTRRCQARLPLPCHECNQRFRHAPFLALHLQVHASAVPDLGFICHLCGHSFRGWVALVLHLRAHSASKRP
  ITCPECNKRFWRQKQLRAHLRRCQPPAPEARPFICGNCGRSFAQWDQLVVHKRVHVAEALEEAAAKALGP
  RPRGRPSVTAPRPGGDAVDRPFQCACCGKRFRHKPNLIAHRRVHTGERPHQCPECGKRFTNKPYLTSHRR
  IHTGEKPYPCTECGRRFRHKPNLLSHSKIHKRSEVSAQAASHTGSHLIAAEPMAQPALGVPLGSLRTPAE
  APASLHSCTDCGRSFRLERFLRLHQRQHTGERPFTCTECGKNFGKKTHLVAHSRVHSGERPFACEECGRR
  FSQGSHLAAHRRDHAPERPFVCPDCGKAFRHKPYLAAHRRIHTGEKPYVCPDCGKAFSQKSNLVSHRRIH
  TGERPYACPDCDRSFSQKSNLITHRKSHIRDGAFCCAICGQTFDDEDRLLMHQKKHDA
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001005893
• ORF Size: 1644 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001005893.1, NP_001005893.1
• RefSeq Size: 1716 bp
• RefSeq ORF: 1647 bp
• Locus ID: 445541
• Cytogenetics: 4q24
• MW: 62 kDa