Mycn (NM_001013096) Rat Tagged ORF Clone

CAT#: RR206566

Mycn (Myc-DDK-tagged ORF) - Rat v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian) (Mycn), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mycn
• Synonyms: N-myc; Nmuc1; Nmyc
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206566 representing NM_001013096
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCAGCTGCACCGCGTCCACCATGCCGGGGATGATCTGCAAGAACCCAGACCTCGAGTTTGACTCCC
  TGCAGCCCTGCTTCTACCCGGACGAAGATGACTTCTACTTCGGCGGTCCCGACTCGACCCCACCAGGGGA
  GGACATCTGGAAGAAGTTTGAGCTGCTGCCCACGCCCCCGTTGTCGCCCAGCCGCGCCTTCCCAGAGCAC
  AGCCCGGAGCCCTCAAATTGGGCCACGGAGATGCTGCTGCCGGAGGCCGACCTGTGGGGCAACCCGGCCG
  AGGAGGATGCGTTTGGTCTGGGGGGACTGGGTGGCCTCACTCCTAATCCGGTCATCCTTCAGGACTGCAT
  GTGGAGCGGCTTCTCTGCCCGCGAGAAGCTGGAGCGCGCAGTGAACGAGAAGCTGCAGCACGGCCACGGG
  CCCCCGGGAGCCAGCTCATCCTGTCCGGCTCCGGGAGTGGGTGCCAGTAGCTCCGGGGGCCGTGCCCTTG
  GTGGGTCGGCGAGTGCTGGTCGCACCGGGGCCACCCTGCCTACCGACCTTTCCCACCCGGCTGCGGAGTG
  TGTGGATCCCGCCGTGGTCTTCCCCTTCCCGGTGAACAAGCGAGAGTCGGCTTCGGTGCCCGCTGCCCCG
  ACCAGTGCCCCGGCGACCAGCGCTGTGGTCACTAGTGTGTCTGTCCCGGCTGTAGCCCCGGTGGCTGCTC
  CTGCTCGTGGCAGCGGTCGTCCTGCCAACAGTGGCGAGCACAAGGCCCTCAGCACCTCTGGAGAGGATAC
  CTTGAGCGACTCAGATGATGAAGACGATGAGGAGGAAGATGAAGAGGAGGAGATCGATGTGGTCACGGTA
  GAGAAGAGACGCTCCTCCTCTAACAACAAGGCAGTCACCACTTTCACCATCACTGTACGCCCCAAGACCT
  CGGCTCTGGGCCTGGGGCGAGCACAACCTGGCGAGCTGATCCTCAAGCGCTGTGTCCCCATCCATCAGCA
  ACACAACTATGCCGCACCCTCACCCTATGTGGAGAGCGAGGATGCGCCACCACAGAAGAAGATCAAGAGC
  GAGGCTTCTCCACGCCCCCTCAAAAGTGTCGTTCCACCAAAGGCTAAGAGCTTGAGCCCCCGAAACTCAG
  ACTCGGAGGACAGCGAGCGCCGCCGAAACCACAACATCCTGGAGCGCCAGCGCCGCAACGACCTGCGCTC
  CAGCTTCCTGACGCTCAGGGACCATGTGCCCGAGCTGGTGAAGAACGAGAAGGCCGCCAAGGTGGTCATC
  TTAAAAAAGGCCACCGAGTACGTCCACGCCCTACAGGCCAACGAGCACCAGCTCCTGCTGGAAAAGGAGA
  AACTGCAGGCGAGGCAGCAGCAGTTGCTAAAGAAGATCGAACACGCTCGGACTTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206566 representing NM_001013096
  Red=Cloning site Green=Tags(s)
  
  MPSCTASTMPGMICKNPDLEFDSLQPCFYPDEDDFYFGGPDSTPPGEDIWKKFELLPTPPLSPSRAFPEH
  SPEPSNWATEMLLPEADLWGNPAEEDAFGLGGLGGLTPNPVILQDCMWSGFSAREKLERAVNEKLQHGHG
  PPGASSSCPAPGVGASSSGGRALGGSASAGRTGATLPTDLSHPAAECVDPAVVFPFPVNKRESASVPAAP
  TSAPATSAVVTSVSVPAVAPVAAPARGSGRPANSGEHKALSTSGEDTLSDSDDEDDEEEDEEEEIDVVTV
  EKRRSSSNNKAVTTFTITVRPKTSALGLGRAQPGELILKRCVPIHQQHNYAAPSPYVESEDAPPQKKIKS
  EASPRPLKSVVPPKAKSLSPRNSDSEDSERRRNHNILERQRRNDLRSSFLTLRDHVPELVKNEKAAKVVI
  LKKATEYVHALQANEHQLLLEKEKLQARQQQLLKKIEHARTC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001013096
• ORF Size: 1386 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001013096.1, NP_001013114.1
• RefSeq Size: 2604 bp
• RefSeq ORF: 1389 bp
• Locus ID: 298894
• Cytogenetics: 6q15
• MW: 49.7 kDa