Rps19 (NM_001037346) Rat Tagged ORF Clone

CAT#: RR206568

Rps19 (Myc-DDK-tagged ORF) - Rat ribosomal protein S19 (Rps19), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rps19
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206568 representing NM_001037346
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTGGAGTTACTGTAAAAGACGTTAACCAGCAGGAGTTCGTCAGAGCTCTGGCAGCCTTCCTCAAAA
  AGTCTGGGAAGCTGAAAGTCCCCGAATGGGTGGACACAGTCAAGTTGGCCAAACATAAAGAGCTTGCCCC
  ATATGATGAGAACTGGTTCTACACACGAGCTGCTTCCACAGCACGGCACCTGTACCTCCGTGGTGGTGCA
  GGGGTTGGTTCCATGACCAAGATCTACGGAGGACGGCAGAGAAACGGTGTCAGGCCCAGCCACTTCAGCA
  GAGGCTCTAAGAGTGTGGCCCGCCGGGTCCTCCAAGCCCTGGAGGGGCTGAAAATGGTGGAAAAGGACCA
  AGATGGGGGCCGCAAGCTAACACCTCAGGGACAGAGAGATCTGGACAGGATCGCCGGACAGGTGGCAGCT
  GCCAACAAGAAGCAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206568 representing NM_001037346
  Red=Cloning site Green=Tags(s)
  
  MPGVTVKDVNQQEFVRALAAFLKKSGKLKVPEWVDTVKLAKHKELAPYDENWFYTRAASTARHLYLRGGA
  GVGSMTKIYGGRQRNGVRPSHFSRGSKSVARRVLQALEGLKMVEKDQDGGRKLTPQGQRDLDRIAGQVAA
  ANKKH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001037346
• ORF Size: 435 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001037346.1, NM_001037346.2, NP_001032423.1
• RefSeq Size: 548 bp
• RefSeq ORF: 438 bp
• Locus ID: 29287
• Cytogenetics: 9q12
• MW: 16.1 kDa
• Gene Summary: a ribosomal protein [RGD, Feb 2006]