Ehd1 (NM_001011939) Rat Tagged ORF Clone

CAT#: RR206638

Ehd1 (Myc-DDK-tagged ORF) - Rat EH-domain containing 1 (Ehd1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ehd1
• Synonyms: RGD1306960
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206638 representing NM_001011939
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCAGCTGGGTGAGCAAGGATGCCCGCCGCAAGAAGGAGCCGGAGCTCTTCCAGACGGTGGCCGAGG
  GGCTGCGGCAGCTGTACGCGCAGAAGCTGCTGCCGCTGGAGGAGCACTATCGCTTCCACGAGTTCCATTC
  GCCGGCTCTGGAGGACGCTGACTTCGACAACAAACCCATGGTGCTCCTGGTCGGCCAGTACAGCACCGGC
  AAGACCACCTTCATCCGCCACTTGATCGAGCAGGACTTCCCAGGGATGCGCATCGGGCCGGAGCCCACCA
  CCGACTCCTTCATCGCAGTCATGCATGGCCCCACCGAGGGCGTGGTGCCCGGCAACGCGCTCGTCGTGGA
  CCCGCGGCGCCCCTTCCGCAAGCTTAACGCCTTCGGCAACGCCTTCCTCAACAGGTTCATGTGTGCACAG
  CTGCCTAACCCAGTATTGGACAGCATTAGCATCATTGACACTCCTGGAATCCTGTCTGGAGAGAAGCAGC
  GCATCAGCCGAGGTTATGACTTTGCGGCTGTCCTTGAGTGGTTCGCAGAGCGTGTGGACCGCATCATTCT
  GCTCTTTGACGCCCACAAGCTAGACATCTCAGATGAATTTTCAGAAGTCATCAAGGCCCTCAAAAATCAC
  GAGGACAAGATCCGTGTGGTGCTGAACAAAGCTGACCAAATTGAGACACAGCAGCTGATGCGAGTGTACG
  GAGCTCTCATGTGGTCGCTGGGGAAGATCATCAACACCCCTGAGGTGGTCCGTGTCTACATTGGCTCCTT
  CTGGTCACACCCACTGCTCATCCCTGACAACCGGAAGCTCTTTGAGGCAGAGGAGCAGGACCTCTTCAAA
  GACATCCAGTCTCTACCAAGAAATGCTGCCCTTAGGAAGCTCAATGACTTAATCAAGCGGGCCAGGCTGG
  CCAAGGTCCATGCCTACATCATCAGCTCCCTCAAGAAGGAGATGCCCAATGTCTTTGGGAAAGAGAGCAA
  GAAGAAAGAGCTGGTGAACAACTTGGGAGAGATCTACCAGAAAATTGAGCGGGAGCACCAGATCTCCTCC
  GGCGACTTCCCAAGCCTGCGTAAGATGCAGGAACTTCTGCAGACGCAGGACTTCAGCAAGTTCCAGGCCT
  TGAAGCCCAAGCTGCTGGATACGGTGGATGATATGCTGGCCAACGATATAGCTCGGCTGATGGTGATGGT
  GCGCCAGGAGGAGTCCCTGATGCCCTCACAGGCTGTGAAGGGTGGTGCTTTTGATGGCACCATGAATGGG
  CCCTTTGGGCATGGGTATGGCGAGGGGGCTGGCGAGGGCATTGATGATGTTGAGTGGGTAGTTGGCAAGG
  ACAAGCCCACCTATGATGAGATCTTCTACACACTGTCTCCTGTCAATGGCAAGATCACGGGCGCTAATGC
  CAAGAAGGAGATGGTGAAGTCCAAGCTGCCTAACACAGTGCTGGGGAAGATCTGGAAGCTGGCTGATGTG
  GACAAGGATGGCCTGCTAGATGACGAGGAGTTTGCCCTGGCCAACCACCTTATCAAGGTCAAGCTAGAGG
  GCCATGAGCTGCCCGCTGACCTTCCTCCACATCTCATCCCACCCTCCAAACGGAGGCATGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206638 representing NM_001011939
  Red=Cloning site Green=Tags(s)
  
  MFSWVSKDARRKKEPELFQTVAEGLRQLYAQKLLPLEEHYRFHEFHSPALEDADFDNKPMVLLVGQYSTG
  KTTFIRHLIEQDFPGMRIGPEPTTDSFIAVMHGPTEGVVPGNALVVDPRRPFRKLNAFGNAFLNRFMCAQ
  LPNPVLDSISIIDTPGILSGEKQRISRGYDFAAVLEWFAERVDRIILLFDAHKLDISDEFSEVIKALKNH
  EDKIRVVLNKADQIETQQLMRVYGALMWSLGKIINTPEVVRVYIGSFWSHPLLIPDNRKLFEAEEQDLFK
  DIQSLPRNAALRKLNDLIKRARLAKVHAYIISSLKKEMPNVFGKESKKKELVNNLGEIYQKIEREHQISS
  GDFPSLRKMQELLQTQDFSKFQALKPKLLDTVDDMLANDIARLMVMVRQEESLMPSQAVKGGAFDGTMNG
  PFGHGYGEGAGEGIDDVEWVVGKDKPTYDEIFYTLSPVNGKITGANAKKEMVKSKLPNTVLGKIWKLADV
  DKDGLLDDEEFALANHLIKVKLEGHELPADLPPHLIPPSKRRHE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001011939
• ORF Size: 1602 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001011939.1, NM_001011939.2, NP_001011939.1
• RefSeq Size: 3250 bp
• RefSeq ORF: 1605 bp
• Locus ID: 293692
• Cytogenetics: 1q43
• MW: 60.6 kDa