Alas2 (NM_013197) Rat Tagged ORF Clone

CAT#: RR206736

Alas2 (Myc-DDK-tagged ORF) - Rat aminolevulinate, delta-, synthase 2 (Alas2), nuclear gene encoding mitochondrial protein, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Alas2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206736 representing NM_013197
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGCAGCAGCTATGTTGCTACGGTCCTGTCCAGTGCTCTCTAAGGGCCCCACAGGCCTCCTAGGCA
  AAGTGGCTAAAACTTACCAGTTCCTATTTGGTATTGGACGCTGCCCCATCCTGGCCACTCAAGGACCAAC
  CTGTTCTCAAATCCATCTTAAGGCAACCAAGGCTGGAGCAGACTCTCCGTCTTGGACTAAGAGCCATTGT
  CCTTTCATGCTGTCAGAACTCCAAGACAGGAAGAGCAAGATTGTGCAGAGGGCAGCTCCAGAAGTCCAAG
  AGGATGTCAAGACTTTCAAGACAGACCTGCTGAGCTTCATGGAGTCAACTACCCGAAGCCAATCAGTGCC
  TCGTTTCCAGGATCCAGAGCAGACTGGAGGGGCACCTCCCCTGCTGATTCAGAATAATATGACTGGAAGC
  CAGGCTTTTGGTTATGACCAATTTTTTAGAGACAAGATCATGGAAAAGAAGCAGGACCACACATACCGTG
  TGTTCAAGACTGTGAATCGCTGGGCTAATGCCTACCCCTTTGCCCAACACTTCTCCGAGGCATCTATGGA
  CTCAAAGGATGTTTCTGTCTGGTGCAGTAATGACTATTTGGGCATAAGCAGGCACCCTCGTGTCTTGCAG
  GCCATAGAGGAGACCCTGAAGAATCATGGAGCTGGAGCTGGGGGCACTCGCAATATCTCAGGTACAAGCA
  AGTTTCATGTGGAGCTTGAACAGGAGCTGGCTGAACTACATCATAAGGACTCGGCTCTGCTCTTCTCCTC
  CTGTTTTGTGGCCAATGATTCTACTCTCTTTACACTGGCCAAGCTTCTGCCAGGGTGTGAGATCTACTCA
  GACGCAGGCAATCATGCCTCCATGATCCAAGGCATTCGTAACAGTGGTGCAGTCAAGTTTGTCTTCAGAC
  ACAATGACCCAGGCCACCTGAAGAAACTTCTTGAGAAGTCCGATCCCAAGACACCAAAAATTGTGGCTTT
  TGAGACTGTTCACTCCATGGATGGTGCCATCTGTCCTCTGGAGGAATTGTGTGATGTGGCCCACCAGTAC
  GGGGCCCTGACCTTCGTGGATGAAGTCCATGCCGTAGGACTATATGGAACCCGGGGTGCAGGGATCGGGG
  AGCGTGATGGAATTATGCACAAGCTTGACATCATCTCTGGAACTCTTGGCAAGGCCTTTGGCTGTGTCGG
  TGGCTATATCGCCAGCACTCGGGACTTGGTGGACATGGTGCGCTCCTACGCTGCAGGCTTCATCTTTACC
  ACTTCACTGCCTCCCATGGTGCTCTCTGGAGCTCTAGAGTCTGTGCGTCTACTCAAGGGAGAGGAGGGTC
  AAGCCCTGAGGCGTGCACACCAGCGCAACGTCAAACATATGCGCCAGCTACTAATGGACAGGGGCTTTCC
  TGTTATCCCCTGTCCCAGCCACATCATCCCCATAAGGGTGGGTAATGCGGCACTCAACAGCAAGATCTGT
  GATCTTCTGCTCGCCAAGCACAGCATCTATGTGCAGGCCATCAACTACCCAACTGTGCCTCGTGGTGAGG
  AGCTGCTGCGCTTGGCCCCCTCCCCCCACCACAGCCCTCAGATGATGGAAAACTTTGTGGAGAAGTTGCT
  GCTGGCCTGGACTGAGGTGGGGTTGCCCCTCCAAGATGTGTCTGTGGCTGCATGCAACTTCTGCCGTCGT
  CCTGTGCACTTTGAGCTTATGAGCGAGTGGGAGCGATCCTACTTTGGGAACATGGGACCCCAATATGTTA
  CTACCTATGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206736 representing NM_013197
  Red=Cloning site Green=Tags(s)
  
  MVAAAMLLRSCPVLSKGPTGLLGKVAKTYQFLFGIGRCPILATQGPTCSQIHLKATKAGADSPSWTKSHC
  PFMLSELQDRKSKIVQRAAPEVQEDVKTFKTDLLSFMESTTRSQSVPRFQDPEQTGGAPPLLIQNNMTGS
  QAFGYDQFFRDKIMEKKQDHTYRVFKTVNRWANAYPFAQHFSEASMDSKDVSVWCSNDYLGISRHPRVLQ
  AIEETLKNHGAGAGGTRNISGTSKFHVELEQELAELHHKDSALLFSSCFVANDSTLFTLAKLLPGCEIYS
  DAGNHASMIQGIRNSGAVKFVFRHNDPGHLKKLLEKSDPKTPKIVAFETVHSMDGAICPLEELCDVAHQY
  GALTFVDEVHAVGLYGTRGAGIGERDGIMHKLDIISGTLGKAFGCVGGYIASTRDLVDMVRSYAAGFIFT
  TSLPPMVLSGALESVRLLKGEEGQALRRAHQRNVKHMRQLLMDRGFPVIPCPSHIIPIRVGNAALNSKIC
  DLLLAKHSIYVQAINYPTVPRGEELLRLAPSPHHSPQMMENFVEKLLLAWTEVGLPLQDVSVAACNFCRR
  PVHFELMSEWERSYFGNMGPQYVTTYA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013197
• ORF Size: 1761 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_013197.1, NP_037329.1
• RefSeq Size: 1899
• RefSeq ORF: 1764
• Locus ID: 25748
• MW: 64.8 kDa
• Gene Summary: erythroid-specific isozyme that catalyzes the conversion of glycine and succinyl-CoA to 5-aminolevulinate [RGD, Feb 2006]