Dsc2 (NM_001033688) Rat Tagged ORF Clone

CAT#: RR206798

Dsc2 (Myc-DDK-tagged ORF) - Rat desmocollin 2 (Dsc2), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dsc2
• Synonyms: MGC124744
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206798 representing NM_001033688
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCAGCGGGATCCTCGCGCTCCGGGAGCCCTGCCCTCGGCCTGGGACACCTGTTGACCCTCGCGA
  TCCTTGCACTTGCCTGTGATGCTTGTAAAGAAGTCGTCCTCCAGGTCCCCTCTGAACTACCTGCTGGGAA
  ATTTGTTGGCAGAGTGAACCTGATGGAGTGCCTTAAATCAGCAAATGTAATTCACTTGAGTGATCCTGAT
  TTCCAAGTCTTCGAAGATGGTTCTGTGTACACAACCAATTCTGTTGTTTTGTCTTCGGGGCAAAGAAGCT
  TTACTATTTGGCTTTTCAACACAGACAGCCAGGAAGAGAGGGAGTTGTCTGTCCATTTAGAGGGCCCTGT
  AGAGGTCCTAAATCAAAGACTGCATACAGAAAAAGTTCTCCGCCGCGCCAAGAGAAGATGGGCTCCCATC
  CCTTGTTCCATGATGGAGAATTCATTGGGTCCCTTCCCACTTTTCCTTCAACAGATCCAATCCGACAAAG
  CTCAAAACTACACCATATACTATTCCATCAGCGGTCCAGGAGTTGATAAAGAACCCGTAGGCTTATTTCG
  TATTGATAGAGATACTGGAAACCTGTATTGCACTGATCGTGTAGATCGAGAACAGTATGACTCCTTTGAG
  CTGACTGCATTAGCAATGACTCCAGATGGGTATATGGTAGAGAACCTGCTGACGCTCCTCATTAAAGTAG
  AGGATGAGAACGATAATTACCCGATCTTTACCCAGAAAGTGTACAGCTTCACAGTTTTGGAAAACAGTCC
  ATTTGGTTCTCACGTCGGGGAGGTCTGTGCAACAGACAAGGATGAGCCGGACACAATGCACACTCGTCTG
  AAGTATTCCATCCTGGAGCAGTCGCCTTCTCCCCCCATGTTATTCACCATGCATCCCAGGACTGGTGTCA
  TCACTACCACCTCCACCCAGCTGGACAGAGAGTTAATTGACAAGTACCAGTTGATAATAAAAGTTCAGGA
  CATGGATGGCCAGTATTTTGGTTTGCAGACAACAGCGACCTGCGTCATTACTATTGGAGATGTGAATGAC
  AACTTGCCAACATTCACGCGTACTACGTACGTGACATCAGTGGAAGAAAATACGGTCAATGTGGAACTCT
  TACGTCTTTCTGTTCAAGATAAAGATTTAGTTAACTCTCCTAATTGGAGAGCTAATTACACCATTTTAAA
  GGGCAACGAAAATGGAAATTTTAAAATCAAAACAGATCCCCAAACCAATGAAGGCATTCTGTGCGTGATT
  ATGCCTCTGGACTATGAAGAACGGCAACGGGTGACCCTGGAAATTGGCGTAGTTAATGAAGCCCCGTACA
  CTAGACAGGCTGGTTCAAAGTCATCCATGAGCACAGCTACAGTGACTGTCAACGTGAAAAATCAGGATGA
  GGGCCCTGAGTGTATCCCTCAAACGCAAACTGTGAGGATTCGAGAAAATGTACCAATTGGTACTAGAAAC
  GATGGGTATAAAGCATATGACCCAGAGACCAAAAACAGCATTGGCATAAGGTACAGGAAGTTAAGTGACC
  CAAAAGGATGGATCACTGTTAATGAAGATTCAGGATCAATCACCATTTTCCGAACTCTGGATCGGGAGGC
  TGAGACCATCAGAAACGGCATCTACAATATTACAGTCCTTGCATTAGACGCAGACGGGAGAAGCTGTACG
  GGAACTCTGGGAATCTTACTTGAAGATGTGAATGACAACGGCCCATTCATACCCAAGCAGACAGTGGTGA
  TATGCAGGGCTACCATGTCCTCTGCTGAAATTGTTGCGGTTGACCCTGATGAGCCAGCAAATGGTCCACC
  ATTTGATTTTAGTTTGGAGAGTTCTGATTCAGAAGTACGGAGGAGGTGGAAGTTGACAAGAATCAATGAT
  ACTGCTGCTAGTCTTTCCTATCAGAATGACCCTGCGTTTGATTCCTACGTAATTCCCGTCCGAGTTACAG
  ACAGGTTTGGTCTGTCCTCAGTCACGTCACTGAATGTCGTTGTATGTGACTGCATCACTGAAAGTGACTG
  CACATACCGCACTGGAGAAAGGACTGGCTATGCTGACGTGAGACTTGGACCATGGGCTATCCTTGCTATA
  CTGCTGGGCGTAGCCTTGCTACTTTGTATCCTGTTTACCTTAGTCTGTAGTGTTTCCCGGGCATCAAAAC
  AACAGAAAATTCTTCCCGATGACTTAGCTCAGCAGAACCTAATTGTATCAAACACCGAGGCTCCTGGAGA
  TGACAAAGTCTATTCCGCAAATGGCTTCACAGCTCAAACTGCGGGAGCTTCTGGGCAGACAACATTCACA
  ACCGTGGGAGCTGGAGTCAAAAGTACAGGGCAAGAGACCATTGAGATGGTAAAAGGAGGACAGCGCACAC
  TGGACTCCCGCAGGGCGACCGGCTATCACCACCATACCATGGATCCTGGCAGGGGAGGGCATGTGGAGGT
  GGACAATTACAGATACACCTACTCTGAGTGGCACAATTTCACTCAGCCCCGACTTGGTGAGAAGGTGCAG
  TTTTGCCATGAGGATGACAACCAGAAGCTTGCCCAAGACTATGTCCTCACATACAACTATGAGGGAAAAG
  GCTCGGCTGCTGGCTCTGTGGGTTGCTGTAGCGACCTACAGGAAGAAGACGGGCTTGAGTTTTTGGACCA
  TCTGGAGCCTAAATTTAGGACATTAGCAGAAGTATGTGCAAAGAGG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206798 representing NM_001033688
  Red=Cloning site Green=Tags(s)
  
  MAAAGSSRSGSPALGLGHLLTLAILALACDACKEVVLQVPSELPAGKFVGRVNLMECLKSANVIHLSDPD
  FQVFEDGSVYTTNSVVLSSGQRSFTIWLFNTDSQEERELSVHLEGPVEVLNQRLHTEKVLRRAKRRWAPI
  PCSMMENSLGPFPLFLQQIQSDKAQNYTIYYSISGPGVDKEPVGLFRIDRDTGNLYCTDRVDREQYDSFE
  LTALAMTPDGYMVENLLTLLIKVEDENDNYPIFTQKVYSFTVLENSPFGSHVGEVCATDKDEPDTMHTRL
  KYSILEQSPSPPMLFTMHPRTGVITTTSTQLDRELIDKYQLIIKVQDMDGQYFGLQTTATCVITIGDVND
  NLPTFTRTTYVTSVEENTVNVELLRLSVQDKDLVNSPNWRANYTILKGNENGNFKIKTDPQTNEGILCVI
  MPLDYEERQRVTLEIGVVNEAPYTRQAGSKSSMSTATVTVNVKNQDEGPECIPQTQTVRIRENVPIGTRN
  DGYKAYDPETKNSIGIRYRKLSDPKGWITVNEDSGSITIFRTLDREAETIRNGIYNITVLALDADGRSCT
  GTLGILLEDVNDNGPFIPKQTVVICRATMSSAEIVAVDPDEPANGPPFDFSLESSDSEVRRRWKLTRIND
  TAASLSYQNDPAFDSYVIPVRVTDRFGLSSVTSLNVVVCDCITESDCTYRTGERTGYADVRLGPWAILAI
  LLGVALLLCILFTLVCSVSRASKQQKILPDDLAQQNLIVSNTEAPGDDKVYSANGFTAQTAGASGQTTFT
  TVGAGVKSTGQETIEMVKGGQRTLDSRRATGYHHHTMDPGRGGHVEVDNYRYTYSEWHNFTQPRLGEKVQ
  FCHEDDNQKLAQDYVLTYNYEGKGSAAGSVGCCSDLQEEDGLEFLDHLEPKFRTLAEVCAKR
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033688
• ORF Size: 2706 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033688.1, NP_001028860.1
• RefSeq Size: 4529 bp
• RefSeq ORF: 2709 bp
• Locus ID: 291760
• Cytogenetics: 18p12
• MW: 100 kDa