Me2 (NM_001107376) Rat Tagged ORF Clone

CAT#: RR206940

Me2 (Myc-DDK-tagged ORF) - Rat malic enzyme 2, NAD(+)-dependent, mitochondrial (Me2), nuclear gene encoding mitochondrial protein, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Me2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206940 representing NM_001107376
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTTCCCGGTTAAGAGCTGTTACCACTCCTTGCACTTTGACCTGCCGCAGAGTGCACCTGAAGGAGA
  AGGGCAAGCCACTCATGTTGAATCCAAGAACAAACAAGGGGATGGCGTTTACATTACAAGAACGACAAAT
  GCTTGGTCTTCAAGGACTTTTACCTCCTAAAATAGAGACACAGGATATTCAAGCCTTACGATTCCATAGA
  AACTTGAAAAAAATGACTAGCCCTTTGGAAAAATACATCTATATAATGGGCATACAAGAAAGAAACGAGA
  AACTGTTTTATAGAATACTACAGGATGATATTGAAAGTCTGATGCCAATTGTGTACACACCAACAGTTGG
  TCTTGCCTGTTGTCAATACGGACATATCTTTAGGAGACCTAAGGGATTGTTTATTTCAATCTCAGACAGA
  GGTCATGTTAGATCAATTGTGGATAACTGGCCAGAAAATCATGTTAAGGCTGTTGTGGTGACTGATGGAG
  AGAGAATCCTGGGACTTGGAGATTTGGGTGTCTATGGAATGGGGATTCCAGTCGGGAAGCTTTGCTTGTA
  TACAGCATGCGCAGGAATCCAGCCTGAGAAATGCCTGCCTGTGTGCATCGATGTAGGGACTGATAACATG
  GCTCTACTGAAAGACCCGTTTTATATGGGCTTGTATCAGAAGCGAGACCGCTCCCAGCTCTATGACGATC
  TGATGGATGAGTTTATGAAGGCGATCACTGACAGATATGGCCGGAACACACTTATTCAGTTTGAAGACTT
  TGGAAATCATAATGCATTCAGATTCTTAAAAAAATACCAGGAAAAATACTGTACCTTCAATGACGACATT
  CAAGGGACTGCTGCAGTTGCCTTGTCTGGTCTCCTTGCGGCCCAAAGAGTGATTAATAAACCAATCTCAG
  AACACAGAATCTTATTCCTCGGGGCAGGAGAGGCTGCACTTGGAATTGCGAAGCTTATAGTCATATCAAT
  GATGGAAAGTGGTCTCTCAGAAGAGGAGGCACAGAGAAAGATCTGGATGTTCGACAAGAACGGCTTGCTA
  GTTAAGGGCCGGACTGCTAGAATAGATAGTAACCAGGAACCATATGCTCATGCTGCCTCAGAGAACATAC
  CTGCAACCTTTGAAGATGCAGTAAATAAACTTAAGCCTTCAGTTATAATTGGAGTGGCAGGTGCTGGCCC
  GCTTTTTACTCCTGGCGTAATTAAAGCCATGGCCGCTATCAACGAGAGGCCCATAATATTTGCTTTAAGC
  AATCCTACAGCACAGGCCGAGTGCACTGCCGAAGACGCTTACACACTTACAGAGGGCAGGTGCTTGTTTG
  CCAGTGGCAGCCCATTTGAGCCGGTGAGACTCCAGGATGGGCGTGTCTTCACCCCGGGCCAAGGGAACAA
  TGCATACATCTTCCCAGGTGTGGCTTTAGCTGTTATTCTCTGTGAAGCCCGACACATCAGTGACACCGTT
  TTCTTAGAAGCTGCAAAGGCACTGACATCCCAATTGACAGATGAAGAGTTAGCCCAAGGGAGGCTTTACC
  CATCACTTGCTAATATTCAGGAAGTTTCTGCTAACATAGCTATTAAAATTGCAGAGTACCTGTACGCTAA
  TAAAATGGCTTTCCGATACCCAGAGCCCGAGGACAAGGCCAAATATGTGAAGGAAAGGATATGGCGGAGT
  AATTATGTTTCCCTGCTGCCAGATGTGTATGATTGGCCAGAGTCGTCATTGAAGCCTCCGCAGATACCAG
  AA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206940 representing NM_001107376
  Red=Cloning site Green=Tags(s)
  
  MFSRLRAVTTPCTLTCRRVHLKEKGKPLMLNPRTNKGMAFTLQERQMLGLQGLLPPKIETQDIQALRFHR
  NLKKMTSPLEKYIYIMGIQERNEKLFYRILQDDIESLMPIVYTPTVGLACCQYGHIFRRPKGLFISISDR
  GHVRSIVDNWPENHVKAVVVTDGERILGLGDLGVYGMGIPVGKLCLYTACAGIQPEKCLPVCIDVGTDNM
  ALLKDPFYMGLYQKRDRSQLYDDLMDEFMKAITDRYGRNTLIQFEDFGNHNAFRFLKKYQEKYCTFNDDI
  QGTAAVALSGLLAAQRVINKPISEHRILFLGAGEAALGIAKLIVISMMESGLSEEEAQRKIWMFDKNGLL
  VKGRTARIDSNQEPYAHAASENIPATFEDAVNKLKPSVIIGVAGAGPLFTPGVIKAMAAINERPIIFALS
  NPTAQAECTAEDAYTLTEGRCLFASGSPFEPVRLQDGRVFTPGQGNNAYIFPGVALAVILCEARHISDTV
  FLEAAKALTSQLTDEELAQGRLYPSLANIQEVSANIAIKIAEYLYANKMAFRYPEPEDKAKYVKERIWRS
  NYVSLLPDVYDWPESSLKPPQIPE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001107376
• ORF Size: 1752 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001107376.2, NP_001100846.2
• RefSeq Size: 2899
• RefSeq ORF: 1755
• Locus ID: 307270
• MW: 65.4 kDa