Pkn1 (NM_017175) Rat Tagged ORF Clone

CAT#: RR207075

Pkn1 (Myc-DDK-tagged ORF) - Rat protein kinase N1 (Pkn1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pkn1
• Synonyms: PAK-1; Prkcl1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207075 representing NM_017175
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGCGACGCCGTGCAGAGTGAACCTCGCAGCTGGTCACTGCTGGAGCAGCTGGGTCTGGCTGGGG
  CAGACCTGGCAGCCCCTGGGGTGCAGCAGCAGCTGGAGTTAGAGCGAGAGCGGCTGAAGCGGGAAATCCG
  AAAAGAGCTGAAGCTGAAGGAGGGCGCTGAGAACCTGAGGCGGGCCACCACTGACCTGGGCCGCAGCTTG
  GCCCCTGTGGAACTGCTGCTGAGGGGCTCCGCTAGACGGCTTGACTTGCTGCACCAGCAGCTGCAGGAGC
  TGCATGCACATGTGGTGCTGCCCGACCCTACAGCGGGGAGTGATGCTCCCCAATCCCTTGCAGAGGGCAG
  CCCTGTCTGCTCATCCACCAACCTGAGCCGAGTGGCTGGCCTGGAGAAGCAGCTGGCCATTGAGCTCAAG
  GTCAAACAGGGGGCAGAAAACATGATCCAGACCTACAGCAATGGCAGCACCAAGGACCGGAAGCTGCTGT
  TGACGGCCCAACAGATGCTGCAGGATAGTAAGACCAAGATTGACATCATCCGCATGCAGCTTCGCCGGGC
  GCTACAAGCACTCCAGGCTGGCCAGCTGGAGAGTCAGGCAGCTCCTGATGAGGCCCACGGAGATCCAGAC
  CTGGGAGCCGTAGAGCTACGCATTGAGGAGCTACGACACCATTTTCGAGTAGAGCATGCAGTGGCAGAAG
  GCGCCAAGAATGTCCTGCGTCTGCTCAGTGCTGCAAAGGCCCCAGACCGCAAAGCAGTCAGCGAGGCTCA
  GGAGAAATTGACTGAGTCCAACCAGAAGCTGGGCTTGCTGCGGGAGTCACTGGAGAGGCGCTTGGGGGAA
  CTGCCTGCTGATCACCCCAAGGGACGCCTGCTTCGGGAGGAGCTCACTGCGGCCTCATCGGCAGCCTTCA
  GTGCAATACTGCCTGGGCCCTTCCCTGCCACTCACTACAGCACCTTGAGCAAGCCTGCACCACTCACAGG
  GACCCTGGAAGTACGAGTGGTGGGCTGCAAAAACCTTCCCGAGACCATCCCTTGGAGCCCTCCCCCCTCA
  GTCGGGGCATCTGGGACCCCCGACAGCCGCACTCCTTTCCTGAGTCGTCCAGCTCGGGGCCTTTACAACC
  GAAGTGGAAGCCTTAGTGGACGGAGCAGCCTCAAGGGGGAGGCAGAGAATTCCACTGAGGTCAGCACCGT
  GCTCAAGCTGGACAACACTGTGGTGGGGCAAACAGCCTGGAAGCCATGCGGCCCCAATGCCTGGGACCAG
  AGCTTCACCCTGGAGCTGGAGAGGGCTCGGGAGCTGGAGTTGGCTGTGTTCTGGCGTGACCAGAGGGGTC
  TGTGTGCTCTCAAATTTCTGAAGTTGGAAGACTTCTTGGACAATGAGAGGCATGAGGTGCAGCTGGACAT
  GGAACCCCAGGGCTGCCTGGTGGCTGAGGTCACCTTCCGTAACCCCATCATCGAGCGGATCCCTAGGCTC
  CAAAGGCAGAAAAAAATTTTCTCCAAGCAGCAAGGGCAGACATTTCAGCGTGCCAGACAGATGAACATCG
  ATGTGGCCACCTGGGTGCGGCTGCTCCGGAGACTCATCCCGAACGCCGTGGCCACTGGCTCCTTCAGCCC
  CAATGCATCTCCAGGCTCTGAGATCCGGAGCACTGGAGACATATCCATGGAGAAATTGAATCTCGGTGCT
  GACTCAGACAGCTCGTCCCAGAAGAGCCCCGCAGGGCTGCCCTCCACCTCATGTAGCCTGAGTTCTCCAA
  CCCACGAATCCACCACCTCTCCAGAGCTGCCTTCAGAGACCCAGGAGACCCCAGGCCCTGGCCTGTGCAG
  TCCCCTGAGGAAGTCGCCCCTGACGCTTGAGGACTTCAAGTTCCTGGCAGTGCTTGGTCGGGGTCACTTT
  GGAAAGGTGCTGCTGTCTGAATTCCACTCCAGTGGGGAGCTCTTTGCCATTAAAGCCTTGAAGAAAGGTG
  ACATTGTAGCCCGGGATGAGGTTGAGAGCCTGATGTGTGAGAAGCGGATTTTGGCGACCGTGACCAGGGC
  AGGACATCCCTTCCTGGTGAACCTTTTCGGCTGTTTCCAGACCCCAGAGCATGTGTGCTTTGTGATGGAG
  TACTCAGCCGGTGGGGACTTGATGCTGCATATCCACAGCGACGTGTTCTCAGAGCCTCGGGCTGTCTTCT
  ATTCGGCCTGTGTGGTGCTGGGACTGCAGTTCCTCCATGAACACAAGATTGTCTACAGGGACCTGAAGTT
  GGACAATTTGCTCCTGGATACTGAGGGCTACGTCAAGATCGCAGACTTTGGCCTCTGCAAGGAGGGGATG
  GGCTATGGGGACCGGACCAGCACATTCTGCGGAACTCCGGAGTTCCTGGCGCCGGAAGTGCTCACAGACA
  CATCCTACACTCGAGCCGTGGACTGGTGGGGACTGGGTGTATTGCTCTATGAGATGCTGGTTGGAGAGTC
  TCCGTTCCCTGGGGACGACGAGGAGGAAGTATTTGACAGCATCGTCAATGATGAGGTTCGTTATCCCCGC
  TTCCTGTCTGCGGAGGCCATCGGCATCATGAGAAGGCTACTGCGGAGGAACCCAGAGCGGAGGTTGGGAT
  CCACTGAGCGTGATGCAGAAGATGTGAAAAAACAGCCTTTCTTCAGGACTCTGGACTGGGATGCCCTGCT
  GGCCCGTCGCCTGCCTCCACCCTTCGTGCCTACACTTTCGGGGCGCACAGACGTCAGCAACTTCGATGAG
  GAGTTCACTGGGGAGGCCCCCACACTGAGCCCTCCCCGGGATGCACGGCCCCTGACAGCTGCGGAGCAGG
  CGGCCTTCCGGGATTTCGACTTTGTGGCAGGAGGCTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207075 representing NM_017175
  Red=Cloning site Green=Tags(s)
  
  MAGDAVQSEPRSWSLLEQLGLAGADLAAPGVQQQLELERERLKREIRKELKLKEGAENLRRATTDLGRSL
  APVELLLRGSARRLDLLHQQLQELHAHVVLPDPTAGSDAPQSLAEGSPVCSSTNLSRVAGLEKQLAIELK
  VKQGAENMIQTYSNGSTKDRKLLLTAQQMLQDSKTKIDIIRMQLRRALQALQAGQLESQAAPDEAHGDPD
  LGAVELRIEELRHHFRVEHAVAEGAKNVLRLLSAAKAPDRKAVSEAQEKLTESNQKLGLLRESLERRLGE
  LPADHPKGRLLREELTAASSAAFSAILPGPFPATHYSTLSKPAPLTGTLEVRVVGCKNLPETIPWSPPPS
  VGASGTPDSRTPFLSRPARGLYNRSGSLSGRSSLKGEAENSTEVSTVLKLDNTVVGQTAWKPCGPNAWDQ
  SFTLELERARELELAVFWRDQRGLCALKFLKLEDFLDNERHEVQLDMEPQGCLVAEVTFRNPIIERIPRL
  QRQKKIFSKQQGQTFQRARQMNIDVATWVRLLRRLIPNAVATGSFSPNASPGSEIRSTGDISMEKLNLGA
  DSDSSSQKSPAGLPSTSCSLSSPTHESTTSPELPSETQETPGPGLCSPLRKSPLTLEDFKFLAVLGRGHF
  GKVLLSEFHSSGELFAIKALKKGDIVARDEVESLMCEKRILATVTRAGHPFLVNLFGCFQTPEHVCFVME
  YSAGGDLMLHIHSDVFSEPRAVFYSACVVLGLQFLHEHKIVYRDLKLDNLLLDTEGYVKIADFGLCKEGM
  GYGDRTSTFCGTPEFLAPEVLTDTSYTRAVDWWGLGVLLYEMLVGESPFPGDDEEEVFDSIVNDEVRYPR
  FLSAEAIGIMRRLLRRNPERRLGSTERDAEDVKKQPFFRTLDWDALLARRLPPPFVPTLSGRTDVSNFDE
  EFTGEAPTLSPPRDARPLTAAEQAAFRDFDFVAGGY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017175
• ORF Size: 2838 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_017175.1, NM_017175.2, NP_058871.2
• RefSeq Size: 3087
• RefSeq ORF: 2841
• Locus ID: 29355
• MW: 104.5 kDa
• Gene Summary: displays phosphorylation dependent protein kinase activity [RGD, Feb 2006]