Cspg4 (NM_031022) Rat Tagged ORF Clone

CAT#: RR207093

Cspg4 (Myc-DDK-tagged ORF) - Rat chondroitin sulfate proteoglycan 4 (Cspg4), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cspg4
• Synonyms: Ng2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207093 representing NM_031022
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTCTCAGCCCGGGACACCCGCTGTCAGCTCCAGCCCTGGCCTTGATTCTTACCTTGGCCTTGTTGG
  TCAGATCTACAGCTCCTGCCTCCTTCTTCGGGGAGAACCACCTGGAGGTGCCTGTGCCCTCAGCCCTGAC
  CAGAGTAGACTTACTGCTCCAGTTCTCCACATCGCAGCCCGAAGCCCTGCTCCTCTTGGCAGCAGGCCAA
  ACAGATCATCTCCTGCTGCAGCTCCAGTCTGGACACCTACAGGTCAGACTTGCCCTGGGACAAAATGAGC
  TGAGTCTGCAGACACCAGCAGACACGGTGCTGAGTGACTCCACAACCCACACCGTAGTGCTCACCGTCTC
  CAACAGCTGGGCTGTGCTGTCTGTTGATGGAGTGTTGAACACCTCTGCCCCCATCCCAAAAGCATCCCAC
  CTCAAAGTCCCCTATGGGCTCTTTGTGGGCTCCTCTGGAAGCCTTGACCTGCCTTACCTGAAGGGAATCA
  GTCGACCCCTGAGGGGCTGCCTCCACTCAGCCATTCTCAATGGCCGCAACCTTCTCCGCCCACTAACCCC
  CGATGTTCATGAGGGTTGTGCTGAAGAATTCTCTGCTGGTGATGAAGTTGGCCTGGGCTTCTCTGGACCC
  CACTCACTGGCTGCCTTCCCTGCCTGGAGCACGCGGGAGGAAGGCACCCTGGAGTTTACCCTCACCACTC
  GGAGTCAGCAAGCACCCCTGGCCTTCCAGGCCGGGGACAAGCGTGGCAACTTTATCTACGTGGACATATT
  TGAGGGCCACTTGCGGGCTGTGGTTGAAAAGGGTCAGGGTACCATGCTGCTTCGTAACAGCGTGCCTGTG
  GCTGACGGGCAGCCCCATGAGGTCAGCGTACACATAGATGTTCACCGGCTGGAAATCTCTGTAGATCAAT
  ACCCTACACGTACTTTCAACCGTGGGGTCCTCAGCTACCTGGAGCCTCGTGGCAGTCTCCTCCTTGGGGG
  GCTCGATACAGAGGCCTCTCGCCACCTCCAAGAACACCGTCTGGGCCTGACACCGGGGGCTGCCAACATC
  TCCCTGGTAGGCTGCATAGAAGATTTCAGTGTTAATGGCAGGAGGCTGGGCCTCCGGGACGCCTGGCTGA
  CCCGTGACATGGCAGCAGGCTGCAGGCCTGAGGAGGATGAGTATGAGGAAGAGGTCTATGGCCCGTTTGA
  AGCTTTCTCCACCCTGGCGCCCGAAGCCTGGCCAGTCATGGATCTGCCAGAGCCGTGTGTTCCTGAGCCG
  GGACTGCCTGCCGTCTTCGCGAACTTCACGCAGCTGCTCACCATTAGTCCCCTGGTCGTGGCCGAGGGTG
  GCACAGCCTGGCTTGAGTGGCGGCACGTGCAGCCCACGCTGGACCTGACAGAGGCGGAGCTGCGCAAGTC
  CCAGGTACTGTTCAGCGTGAGCCAGGGTGCACGCCACGGCGAGCTGGAGCTAGACATCCCGGGAGCCCAA
  ACCCGAAAAATGTTTACACTGTTGGACGTGGTGAACCGAAAGGCTCGCTTTGTTCACGATGGCTCTGAAG
  ACACCTCTGACCAGCTGATGCTGGAGGTATCAGTGACTTCTCGGGCACCTGTGCCCTCTTGCCTGCGGAG
  GGGCCAAATTTACATTCTCCCCATCCAGGTAAACCCCGTCAACGACCCACCTCGCATCGTCTTCCCGCAC
  GGCAGCCTCATGGTGATCCTGGAGCACACACAGAAGCCTCTGGGACCCGAGATTTTCCAGGCCTATGACC
  CAGACTCTGCTTGTGAGGGTCTCACCATCCAGCTTCTTGGTGTCTCCGCTAGTGTCCCTGTGGAACACCG
  AGACCAGCCCGGAGAGCCAGTGACTGAGTTTTCCTGTCGAGACCTAGAGGCAGGCAACATAGTCTATGTC
  CACCGTGGCGGGCCTGCACAGGACCTGACATTCCGGGTCAGTGATGGAATGCAGGCCAGTGGGCCAGCTA
  CACTGAAGGTCGTGGCCGTCCGGCCAGCCATACAGATCCTCCACAACACAGGGCTGCGCCTGGCCCAGGG
  CTCTGCTGCAGCCATCTTGCCTGCCAACCTGTCGGTAGAAACGAATGCAGTAGGACAGGATGTGAGCGTG
  CTGTTCCGAGTCACTGGCACCTTGCAGTTTGGGGAGCTGCAGAAGCAGGGGGCCGGAGGGGTAGAGGGCA
  CCGAGTGGTGGGATACACTGGCCTTCCACCAGCGCGATGTGGAGCAAGGCCGAGTGAGGTACCTGAGTAC
  TGACCCACAACACCACACCCAAGACACAGTGGAGGACCTGACCTTGGAGGTGCAGGTGGGCCAGGAGACA
  CTGAGCAATCTGTCTTTCCCAGTGACCATCCAGAGAGCCACAGTATGGATGCTGCAGCTTGAGCCTCTGC
  ATACACAGAACCCTCATCAGGAAACCCTCACCTCAGCCCACCTAGAGGCTTCCCTGGAGGAGGAGGGAGA
  AGGAGGACCATACCCTCATATCTTCCACTATGAGTTGGTTCAGGCCCCCAGGAGAGGCAACCTCCTGCTC
  CAGGGTACAAGGCTGTCAGATGGCCAGAGCTTCAGCCAGAGTGACCTGCAGGCCGGTCGTGTGACCTACA
  GGGCCACAACGCGGACCTCTGAGGCAGCTGAGGACTCCTTCCGTTTCCGGGTCACATCTCCACCACATTT
  CTCCCCGCTCTACACCTTCCCTATCCACATTGGCGGTGACCCAAACGCTCCTGTCCTCACTAACGTCCTG
  CTCATGGTACCCGAGGGAGGGGAGGGAGTGCTGTCTGCTGACCACCTCTTTGTCAAGAGTCTCAACAGTG
  CCAGCTATCTCTATGAGGTTATGGAGCAGCCCCACCATGGCAGCTTGGCTTGGAGGGACCCAAAAGGTAG
  AGCCACCCCAGTAACATCCTTTACTAATGAGGACCTGCTACACGGCCGACTGGTCTACCAGCACGATGAC
  TCTGAGACCATAGAGGATGATATCCCATTTGTGGCCACACGCCAGGGCGAGGGCAGCGGTGACATGGCCT
  GGGAGGAGGTGCGTGGTGTCTTCCGAGTGGCCATCCAGCCTGTGAACGATCACGCCCCTGTGCAGACCAT
  CAGCCGTGTCTTCCACGTGGCCCGGGGCGGACAGCGGCTGTTGACTACAGATGATGTGGCCTTCAGTGAT
  GCTGATTCGGGCTTCAGTGACGCGCAACTGGTGCTGACCCGCAAGGACCTCCTCTTTGGCAGCATCGTGG
  CTATGGAGGAGCCCACGAGGCCCATCTACCGTTTCACCCAAGAGGATCTCAGGAAGAAGCAAGTCCTGTT
  TGTGCACTCAGGGGCCGACCATGGCTGGCTCCAGCTACAGGTGTCTGATGGGCAACACCAGGCTACTGCC
  ATGCTGGAGGTGCAGGCCTCAGAGCCCTATCTCCACGTAGCCAATAGTTCCAGTCTTGTGGTTCCTCAAG
  GAGGCCAGGGCACCATCGACACAGCCGTGCTCCACCTGGACACCAACCTAGATATACGAAGTGGGAATGA
  GGTCCACTACCATGTCACAGCTGGCCCTCACTGGGGACAGCTGCTCCGGGATGGCCAGTCAGTCACCTCC
  TTCTCGCAACGGGACTTGCTGGATGGGGCCATTCTCTACAGCCACAATGGCAGCCTCAGCCCGCAAGACA
  CCCTGGCCCTTTCTGTGGCAGCAGGGCCAGTACACACTAGCACCGTCCTACAAGTGACCATTGCCCTAGA
  GGGCCCCCTGGCTCCACTACAACTGGTGCAGCACAAAAGGATCTATGTCTTCCAAGGGGAGGCAGCTGAG
  ATCAGAAGGGACCAGCTAGAGGTAGTCCAGGAGGCAGTGCTGCCTGCCGACATCATGTTCTCGTTGAGAA
  GCCCCCCGAACGCTGGCTACTTGGTGATGGTGTCCCACGGTGCTTCAGCAGATGGGCCACCCAGCCTGGA
  CCCTGTGCAGCGCTTCTCCCAAGAGGCAATAAATTCAGGCCGGGTTCTCTACTTGCACTCTCGCCCTGGA
  GCCTGGAGTGATTCCTTCTCCCTGGATGTGGCCTCGGGTCTGGGCGATCCTCTCGAAGGCATCTCTGTGG
  AGCTGGAAGTGTTGCCCACCGTCATCCCCCTGGATGTTCAAAACTTCAGCGTTCCTGAGGGTGGCACTCG
  TACGCTGGCCCCTCCGCTGATCCAAATCACTGGGCCCTACTTGGGGACACTGCCAGGCCTTGTCCTACAG
  GTGCTAGAGCCACCGCAGCACGGGGCCCTGCAGAAGGAGGACCGTCCTCAAGATGGGACCCTCAGCACTT
  TCTCCTGGAGAGAGGTGGAAGAGCAGCTGATCCGATACGTGCATGATGGGAGTGAGACGCAGACGGACGG
  CTTCATCCTGCTAGCTAATGCCTCAGAGATGGATCGCCAGAGCCAGCCCATGGCCTTCACTATCACCATC
  CTCCCTGTTAATGACCAACCCCCTGTCATCACCACAAACACAGGCTTGCAGATCTGGGAGGGGGCTATTG
  TGCCCATCCCTCCTGAGGCCCTCAGGGGAATAGACAGTGATTCAGGGCCGGAAGACTTGGTCTACACCAT
  CGAGCAGCCCAGCAATGGACGGATAGCCTTGAGGGTAGCACCAGACGCCGAGGCCCACCGCTTCACACAG
  GCCCAGCTGGACAGCGGGCTTGTGCTGTTCTCACACAGAGGAGCCCTGGAAGGAGGCTTCCACTTCGACC
  TCTCTGATGGCGTACACACTTCTCCTGGACATTTCTTCCGAGTGGTAGCCCAGAAGCAGGTACTCCTCTC
  CTTGGAGGGCAGCCGGAAGCTGACTGTCTGTCCGGAGTCTGTGCAGCCACTCAGCAGCCAGAGCTTGAGT
  GCCAGCTCCAGTACAGGCTCCGACCCTCGTCACCTGCTCTACCAGGTGGTAAGAGGCCCCCAGCTCGGCC
  GACTCCTCCATGCCCAGCAAGGAAGTGCAGAGGAGGCTTTGGTGAACTTCACCCAGGCTGAGGTGAATGC
  TGGGAATATTCTGTATGAGCATGAGATATCCTCTGAGCCCTTCTGGGAAGCCCACGATACCATTGGTCTC
  CTGCTGTCTTCATCACCTGCGAGGGATCTGGCTGCCACCCTGGCTGTGACTGTGTCTTTCGACGCTGCCT
  GTCCCCAGCGGCCTAGCCGTCTCTGGAGGAACAAAGGTCTTTGGGTCCCTGAGGGCCAGCGGGCCAAGAT
  CACTGTGGCTGCCCTTGATGCCGCCAACCTCCTAGCCAGTGTGCCAGCATCTCAGCGCGGTCGGCATGAT
  GTACTCTTCCAGATCACACAGTTCCCCACCCGGGGTCAGCTCCTGGTGTCCGAGGAGCCACTCCATGCCA
  GGAGACCCCACTTCCTGCAATCTGAGCTGACTGCAGGACAGCTGGTGTATGCCCACGGTGGTGGGGGCAC
  CCAGCAGGATGGCTTCCGCTTCCGTGCCCACCTCCAGGGACCAACGGGAGCTTCCGTGGCAGGACCCCAA
  ACCTCTGAGGCTTTTGTCATCACTGTGAGGGACGTGAATGAGCGACCCCCTCAGCCACAGGCCTCCATTC
  CTCTCCGCATCACCAGGGGCTCACGAGCCCCTGTCTCTCGAGCCCAGCTGAGTGTCGTAGACCCAGACTC
  AGCTCCAGGGGAAATTGAGTACGAAGTACAGAGGGCACCCCACAATGGCTTCCTGAGCCTGGCTGGGGAC
  AACACTGGGCCTGTGACTCACTTTACACAGGCTGATGTGGATGCAGGGCGACTGGCCTTTGTGGCAAATG
  GGAGCAGTGTGGCTGGCGTCTTCCAGTTGAGCATGTCTGATGGAGCCAGCCCCCCAATACCCATGTCGCT
  GGCTGTGGATGTCTTGCCATCCACCATTGAGGTGCAGCTGCGAGCGCCCCTGGAGGTGCCTCAAGCCCTA
  GGACGTTCCTCACTGAGCAGGCAGCAGCTCCAGGTGATTTCAGATCGTGAGGAGCCAGATGTAGCTTACC
  GCCTCACTCAGGGGCCCCTGTATGGGCAGGTACTAGTGGGGGGGCAGCCCGCTTCAGCCTTCAGCCAGCT
  GCAGGTAGACCAGGGGGACGTGGTCTTTGCCTTCACCAACTTTTCTTCCTCTCAGGATCATTTCAAAGTC
  CTGGCTCTTGCTAGGGGTGTGAATGCATCAGCTACTGTAAATGTCACAGTGCAGGCCCTGTTGCATGTGT
  GGGCCGGTGGGCCATGGCCTCAGGGTACCACCTTGCGCCTTGACCCCACTGTGCTCGATGCCAGTGAACT
  GGCCAACCGCACAGGCAGTATGCCCCGCTTCCGGCTCCTGGAAGGACCCCGGTACGGCCGTGTGGTTCGT
  GTGTCCCAAGGCCGAGCAGAATCTAGGACCAACCAACTTGTGGAAGATTTCACACAGCAAGACCTGGAAG
  AGGGAAGGCTGGGGCTAGAGGTGGGCAGGCCAGAGGGCAGGTCCACTGGCCCAACAGGTGATAGACTTAC
  CCTGGAGCTGCAGGCAACGGGTGTCCCACCTGCTGTGGCCTTGCTGGACTTTGCCACTGAGCCTTACCAT
  GCAGCCAAGTTCTATAAGGTGACCCTACTCAGTGTCCCTGAGGCTGCTCGTACAGAAACAGAGAAAACAG
  GAAAAAGCACCCCCACTGGCCAGCCAGGCCAAGCAGCATCTAGCCCCATGCCCACTGTAGCCAAAAGTGG
  TTTCTTGGGCTTCTTAGAGGCCAACATGTTCAGCGTCATCATCCCCGTGTGCCTGGTCCTCCTGCTCCTG
  GCTCTCATCTTGCCTCTGCTTTTCTACCTCCGCAAACGCAACAAGACAGGTAAGCATGATGTCCAGGTGT
  TGACCGCCAAACCCCGCAATGGCCTAGCTGGTGACACAGAGACCTTTCGAAAGGTGGAGCCAGGCCAAGC
  CATTCCACTCACAACTGTACCTGGCCAGGGGCCCCCACCCGGAGGCCAGCCTGACCCAGAACTATTACAG
  TTTTGCCGGACACCCAATCCTGCCCTCAGGAATGGCCAGTACTGGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207093 representing NM_031022
  Red=Cloning site Green=Tags(s)
  
  MLLSPGHPLSAPALALILTLALLVRSTAPASFFGENHLEVPVPSALTRVDLLLQFSTSQPEALLLLAAGQ
  TDHLLLQLQSGHLQVRLALGQNELSLQTPADTVLSDSTTHTVVLTVSNSWAVLSVDGVLNTSAPIPKASH
  LKVPYGLFVGSSGSLDLPYLKGISRPLRGCLHSAILNGRNLLRPLTPDVHEGCAEEFSAGDEVGLGFSGP
  HSLAAFPAWSTREEGTLEFTLTTRSQQAPLAFQAGDKRGNFIYVDIFEGHLRAVVEKGQGTMLLRNSVPV
  ADGQPHEVSVHIDVHRLEISVDQYPTRTFNRGVLSYLEPRGSLLLGGLDTEASRHLQEHRLGLTPGAANI
  SLVGCIEDFSVNGRRLGLRDAWLTRDMAAGCRPEEDEYEEEVYGPFEAFSTLAPEAWPVMDLPEPCVPEP
  GLPAVFANFTQLLTISPLVVAEGGTAWLEWRHVQPTLDLTEAELRKSQVLFSVSQGARHGELELDIPGAQ
  TRKMFTLLDVVNRKARFVHDGSEDTSDQLMLEVSVTSRAPVPSCLRRGQIYILPIQVNPVNDPPRIVFPH
  GSLMVILEHTQKPLGPEIFQAYDPDSACEGLTIQLLGVSASVPVEHRDQPGEPVTEFSCRDLEAGNIVYV
  HRGGPAQDLTFRVSDGMQASGPATLKVVAVRPAIQILHNTGLRLAQGSAAAILPANLSVETNAVGQDVSV
  LFRVTGTLQFGELQKQGAGGVEGTEWWDTLAFHQRDVEQGRVRYLSTDPQHHTQDTVEDLTLEVQVGQET
  LSNLSFPVTIQRATVWMLQLEPLHTQNPHQETLTSAHLEASLEEEGEGGPYPHIFHYELVQAPRRGNLLL
  QGTRLSDGQSFSQSDLQAGRVTYRATTRTSEAAEDSFRFRVTSPPHFSPLYTFPIHIGGDPNAPVLTNVL
  LMVPEGGEGVLSADHLFVKSLNSASYLYEVMEQPHHGSLAWRDPKGRATPVTSFTNEDLLHGRLVYQHDD
  SETIEDDIPFVATRQGEGSGDMAWEEVRGVFRVAIQPVNDHAPVQTISRVFHVARGGQRLLTTDDVAFSD
  ADSGFSDAQLVLTRKDLLFGSIVAMEEPTRPIYRFTQEDLRKKQVLFVHSGADHGWLQLQVSDGQHQATA
  MLEVQASEPYLHVANSSSLVVPQGGQGTIDTAVLHLDTNLDIRSGNEVHYHVTAGPHWGQLLRDGQSVTS
  FSQRDLLDGAILYSHNGSLSPQDTLALSVAAGPVHTSTVLQVTIALEGPLAPLQLVQHKRIYVFQGEAAE
  IRRDQLEVVQEAVLPADIMFSLRSPPNAGYLVMVSHGASADGPPSLDPVQRFSQEAINSGRVLYLHSRPG
  AWSDSFSLDVASGLGDPLEGISVELEVLPTVIPLDVQNFSVPEGGTRTLAPPLIQITGPYLGTLPGLVLQ
  VLEPPQHGALQKEDRPQDGTLSTFSWREVEEQLIRYVHDGSETQTDGFILLANASEMDRQSQPMAFTITI
  LPVNDQPPVITTNTGLQIWEGAIVPIPPEALRGIDSDSGPEDLVYTIEQPSNGRIALRVAPDAEAHRFTQ
  AQLDSGLVLFSHRGALEGGFHFDLSDGVHTSPGHFFRVVAQKQVLLSLEGSRKLTVCPESVQPLSSQSLS
  ASSSTGSDPRHLLYQVVRGPQLGRLLHAQQGSAEEALVNFTQAEVNAGNILYEHEISSEPFWEAHDTIGL
  LLSSSPARDLAATLAVTVSFDAACPQRPSRLWRNKGLWVPEGQRAKITVAALDAANLLASVPASQRGRHD
  VLFQITQFPTRGQLLVSEEPLHARRPHFLQSELTAGQLVYAHGGGGTQQDGFRFRAHLQGPTGASVAGPQ
  TSEAFVITVRDVNERPPQPQASIPLRITRGSRAPVSRAQLSVVDPDSAPGEIEYEVQRAPHNGFLSLAGD
  NTGPVTHFTQADVDAGRLAFVANGSSVAGVFQLSMSDGASPPIPMSLAVDVLPSTIEVQLRAPLEVPQAL
  GRSSLSRQQLQVISDREEPDVAYRLTQGPLYGQVLVGGQPASAFSQLQVDQGDVVFAFTNFSSSQDHFKV
  LALARGVNASATVNVTVQALLHVWAGGPWPQGTTLRLDPTVLDASELANRTGSMPRFRLLEGPRYGRVVR
  VSQGRAESRTNQLVEDFTQQDLEEGRLGLEVGRPEGRSTGPTGDRLTLELQATGVPPAVALLDFATEPYH
  AAKFYKVTLLSVPEAARTETEKTGKSTPTGQPGQAASSPMPTVAKSGFLGFLEANMFSVIIPVCLVLLLL
  ALILPLLFYLRKRNKTGKHDVQVLTAKPRNGLAGDTETFRKVEPGQAIPLTTVPGQGPPPGGQPDPELLQ
  FCRTPNPALRNGQYWV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031022
• ORF Size: 6978 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_031022.1, NP_112284.1
• RefSeq Size: 8074
• RefSeq ORF: 6981
• Locus ID: 81651
• MW: 251.9 kDa
• Gene Summary: extracellular matrix protein secreted by many cell types; member of the large chondroitin sulfate proteoglycan (CSPG) family; may be involved with angiogenesis -dependent processes such as wound healing or tumor growth [RGD, Feb 2006]