Dbt (NM_053312) Rat Tagged ORF Clone

CAT#: RR207119

Dbt (Myc-DDK-tagged ORF) - Rat dihydrolipoamide branched chain transacylase E2 (Dbt), nuclear gene encoding mitochondrial protein, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dbt
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207119 representing NM_053312
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCAGCGCGTGTGCTGGGGACCTGGAGCCGGAACGCTGTCAGATTGACCTGTGTTCGCTATTTTC
  AAACATATAATAACGTACACATTTTGAAGCCACAACACGTGTGCTCTGTGGGTTATCCATTGCTCAAGTC
  CAGTCAGCCACGTCACTCCCTGAGAACAGCAGCCGTGCTCCAGGGACAGGTTGTTCAATTCAAACTCTCC
  GACATCGGAGAAGGGATTCGAGAAGTAACCATTAAAGAATGGTATGTAAAAGAAGGAGATACGGTGTCTC
  AGTTTGACAGCATCTGTGAAGTTCAAAGTGACAAGGCCTCTGTCACCATCACCAGCCGTTACGATGGCGT
  CATTAAGAGGCTCTATTACAATCTGGACGATATCGCTTATGTGGGGAAACCACTGATAGACATCGAGACA
  GAAGCTTTAAAGGATTCAGAAGAAGATGTGGTCGAAACTCCTGCTGTAGCCCATGATGAACACACCCACC
  AAGAGATAAAAGGCCAGAAAACACTAGCAACGCCTGCAGTTCGTCGGCTGGCAATGGAAAACAATATCAA
  GCTGAGTGAAGTTGTTGGCTCAGGAAAGGATGGCAGAATACTTAAAGAAGACATTCTCAACTTTTTGGAA
  AAGCAGACAGGAGCCATACTGCCTCCCTCACCGAAATCGGAAATCACACCACCGCCACCACAGCCCAGAG
  ACAGGCCCTTTCCCACGCCGGTATCAAAGCCTCCAGTATTCCTGGGCAAAGACCGAACAGAGCCTGTGAC
  AGGCTTCCAAAAGGCAATGGTCAAGACCATGTCCGCAGCCCTGAAGATTCCTCACTTTGGGTACTGTGAT
  GAGGTTGACCTTACTGAACTGGTTAAGCTTCGAGAAGAATTGAAACCTGTTGCTTTGGCTCGTGGGATCA
  AACTCTCCTTCATGCCGTTCTTCTTAAAGGCTGCGTCTTTGGGACTACTGCAGTTCCCTATCCTTAATGC
  CTCCGTGGATGAAAACTGCCAGAGCATCACCTACAAGGCCTCTCACAACATTGGGATAGCCATGGATACC
  GAGCGGGGGTTAATTGTCCCCAACGTGAAGAACGTTCAGGTGCGCTCTGTATTTGAGATCGCCATGGAAC
  TGAACCGCCTCCAGAAACTGGGCTCTTTGGGTCAGCTCAGTACCACAGACCTCACGGGAGGGACATTCAC
  TCTTTCCAACATTGGATCAATTGGTGGAACCTATGCCAAGCCAGTGATATTGCCACCTGAAGTAGCCATT
  GGCGCCCTGGGAGCCATTAAGGCTCTTCCCCGATTTGACCAGAAAGGGGATGTTTACAAAGCCCAGATAA
  TGAATGTGAGCTGGTCAGCAGATCACAGGGTCATTGACGGCGCCACCATGTCACGATTCTCTAACTTGTG
  GAAATCCTACTTAGAAAACCCAGCGTTCATGCTGCTGGATCTGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207119 representing NM_053312
  Red=Cloning site Green=Tags(s)
  
  MAAARVLGTWSRNAVRLTCVRYFQTYNNVHILKPQHVCSVGYPLLKSSQPRHSLRTAAVLQGQVVQFKLS
  DIGEGIREVTIKEWYVKEGDTVSQFDSICEVQSDKASVTITSRYDGVIKRLYYNLDDIAYVGKPLIDIET
  EALKDSEEDVVETPAVAHDEHTHQEIKGQKTLATPAVRRLAMENNIKLSEVVGSGKDGRILKEDILNFLE
  KQTGAILPPSPKSEITPPPPQPRDRPFPTPVSKPPVFLGKDRTEPVTGFQKAMVKTMSAALKIPHFGYCD
  EVDLTELVKLREELKPVALARGIKLSFMPFFLKAASLGLLQFPILNASVDENCQSITYKASHNIGIAMDT
  ERGLIVPNVKNVQVRSVFEIAMELNRLQKLGSLGQLSTTDLTGGTFTLSNIGSIGGTYAKPVILPPEVAI
  GALGAIKALPRFDQKGDVYKAQIMNVSWSADHRVIDGATMSRFSNLWKSYLENPAFMLLDLK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053312
• ORF Size: 1446 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_053312.1, NP_445764.1
• RefSeq Size: 3166
• RefSeq ORF: 1449
• Locus ID: 29611
• MW: 53.3 kDa
• Gene Summary: a component of the branched-chain alpha-keto acid dehydrogenase complex; involved in the catabolism of the branched-chain amino acids, isoleucine, leucine, and valine [RGD, Feb 2006]