Ctps2 (NM_001034998) Rat Tagged ORF Clone

CAT#: RR207270

Ctps2 (Myc-DDK-tagged ORF) - Rat CTP synthase II (Ctps2), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ctps2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207270 representing NM_001034998
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGTATATCCTGGTTACTGGTGGGGTCATCTCAGGCATTGGTAAAGGGATCATCGCCAGCAGCATTG
  GGACCATTCTCAAGTCATGTGGACTCCGAGTAACCGCCATCAAGATCGACCCCTACATTAACATTGATGC
  TGGCACTTTCTCACCTTACGAACATGGTGAAGTATTTGTTTTAAATGATGGTGGAGAAGTTGATTTGGAC
  CTTGGAAATTATGAAAGATTCTTGGACATTAATCTTTATAAAGACAACAACATCACCACTGGGAAGATAT
  ATCAGCATGTGATCAACAAAGAGAGGCGCGGTGATTATCTGGGGAAAACTGTCCAAGTGGTCCCCCACAT
  TACTGATGCTATCCAAGACTGGGTTATGAATCAAGCCAAGGTGTCTGTGGATGGCAATAAGGAAGATCCC
  CAGATATGTGTTATCGAGCTGGGAGGCACCATTGGAGACATCGAAGGGATGGCATTTGTGGAAGCATTCC
  GACAATTCCAGTTTAAAGCAAAGAGAGAGAATTTCTATAATATTCATGTCAGCCTTGTGCCACAGCCCAG
  TGCTACTGGAGAACAAAAAACCAAGCCTACACAAAACAGTGTCCGTGCGCTGAGAGGGCTGGGCTTGTCT
  CCGGATCTGATTGTCTGCCGAAGTTCAACACCCATTGAGATGGCTGTGAAGGAGAAGATTTCTATGTTTT
  GTCACGTGAACCCTGAACAGGTCATCTGTATCCATGATGTTTCTTCCATCTACCGAGTCCCTCTTCTTTT
  GGAGGAGCAAGGTGTGGTTAAATATTTTCAAGAGAGATTGGACCTGCCCATCAATGATTGCTCAAATAAT
  TTGCTTTTCAAGTGGAAAACCATGGCTGACAGGTATGAAAGATTGCAGAAAATATGTTCCATAGCCCTGG
  TTGGTAAATACACCAAACTCAGGGACTGCTACGCCTCTGTGTTCAAAGCCCTGGAACACTCAGCCTTGGC
  CATCAACCACAAGTTGAATCTGATGTACATAGACTCCATTGATCTGGAGCCAGTCACCAAAGCTGAGGAT
  CCTGTGAAATTCCATGAAGCTTGGCAGAAGCTGTGCTTAGCTGATGGTATTCTCGTGCCTGGAGGCTTTG
  GAATCCGAGGAACATTGGGAAAACTCCAGGCAATTTCTTGGGCAAGGACAAAGAAGATTCCATTTCTGGG
  AATTTGCCTTGGGATGCAACTGGCAGTGATTGAGTTTGCAAGAAACTGCCTGAACTTGAAAGATGCTAAT
  TCTACAGAATTTGATCCAAACACTCCAGTCCCATTGGTAATTGACATGCCTGAACACAACCCTGGCGATT
  TGGGAGGAACAATGAGACTGGGATTAAGACGAACTGTTTTCACAACTGAAAATTCCATACTGAAGAAGCT
  TTACGGTGATGTTCCTTACATAGAAGAAAGACACCGACATCGGTATGAGGTGAACCCTAACCTGATCAAC
  CAATTTGAGAATAAAGACCTTTGCTTTGTCGGTGAGGATGTGGATGGGAAGAGGATGGAGATTATTGAAT
  TGACAGGTCATCCATATTTCATCGGCGTCCAATTCCATCCTGAGTTTTCCTCTAGGCCAATGAAGCCTTC
  CCCTCCGTACCTGGGACTCTTACTTGCAGCAACCGGAACTCTGAATACCCATCTGCAACAGATGAGCAAA
  CTATCGTACAGTGACATATACAGTGATGCCAGTGATGATAGCTTTTCAGAGGCGAAGTTTGCTGAGCTGG
  ATATCAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207270 representing NM_001034998
  Red=Cloning site Green=Tags(s)
  
  MKYILVTGGVISGIGKGIIASSIGTILKSCGLRVTAIKIDPYINIDAGTFSPYEHGEVFVLNDGGEVDLD
  LGNYERFLDINLYKDNNITTGKIYQHVINKERRGDYLGKTVQVVPHITDAIQDWVMNQAKVSVDGNKEDP
  QICVIELGGTIGDIEGMAFVEAFRQFQFKAKRENFYNIHVSLVPQPSATGEQKTKPTQNSVRALRGLGLS
  PDLIVCRSSTPIEMAVKEKISMFCHVNPEQVICIHDVSSIYRVPLLLEEQGVVKYFQERLDLPINDCSNN
  LLFKWKTMADRYERLQKICSIALVGKYTKLRDCYASVFKALEHSALAINHKLNLMYIDSIDLEPVTKAED
  PVKFHEAWQKLCLADGILVPGGFGIRGTLGKLQAISWARTKKIPFLGICLGMQLAVIEFARNCLNLKDAN
  STEFDPNTPVPLVIDMPEHNPGDLGGTMRLGLRRTVFTTENSILKKLYGDVPYIEERHRHRYEVNPNLIN
  QFENKDLCFVGEDVDGKRMEIIELTGHPYFIGVQFHPEFSSRPMKPSPPYLGLLLAATGTLNTHLQQMSK
  LSYSDIYSDASDDSFSEAKFAELDIN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001034998
• ORF Size: 1758 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001034998.1, NM_001034998.2, NP_001030170.1
• RefSeq Size: 3272 bp
• RefSeq ORF: 1761 bp
• Locus ID: 619580
• Cytogenetics: Xq14
• MW: 65.7 kDa