Cldn19 (NM_001008514) Rat Tagged ORF Clone

CAT#: RR207272

Cldn19 (Myc-DDK-tagged ORF) - Rat claudin 19 (Cldn19), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cldn19
• Synonyms: claudin-19
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207272 representing NM_001008514
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAACTCAGGCCTCCAGCTCCTGGGCTACTTCCTAGCCTTGGGTGGCTGGGTGGGCATCATCGCCA
  GCACCGCCCTGCCACAGTGGAAGCAGTCCTCCTATGCAGGCGATGCCATCATCACTGCCGTGGGCCTCTA
  CGAAGGGCTGTGGATGTCCTGCGCCTCTCAGAGCACCGGGCAGGTGCAATGCAAACTCTACGATTCACTC
  CTGGCCCTGGACGGTCATATCCAGTCAGCAAGAGCCCTGATGGTGGTGGCTGTGCTCCTGGGCTTTGTGG
  CCATGGTGCTCAGTGTCGTGGGCATGAAGTGTACTCGGGTTGGAGACAGTAACCCCACTGCCAAGGGCCG
  TGTGGCCATCTCCGGAGGTGCTCTCTTCCTCTTGGCGGGTCTCTGTACTTTGACTGCTGTCTCATGGTAC
  GCTACCCTGGTGACCCAGGAATTCTTCAACCCCAGCACTCCTGTCAACGCCAGGTATGAATTTGGTCCAG
  CTCTGTTCGTCGGCTGGGCCTCTGCTGGGCTGGCCATCCTGGGCGGTTCCTTTCTCTGCTGCACATGCCC
  AGAGCCTGAGAGAGCCAACAGCATCCCGCAGCCCTATCGCTCTGGACCCTCAACGGCTGCCCGAGAGTAC
  GTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207272 representing NM_001008514
  Red=Cloning site Green=Tags(s)
  
  MANSGLQLLGYFLALGGWVGIIASTALPQWKQSSYAGDAIITAVGLYEGLWMSCASQSTGQVQCKLYDSL
  LALDGHIQSARALMVVAVLLGFVAMVLSVVGMKCTRVGDSNPTAKGRVAISGGALFLLAGLCTLTAVSWY
  ATLVTQEFFNPSTPVNARYEFGPALFVGWASAGLAILGGSFLCCTCPEPERANSIPQPYRSGPSTAAREY
  V
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001008514
• ORF Size: 633 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001008514.1, NP_001008514.1
• RefSeq Size: 1626 bp
• RefSeq ORF: 636 bp
• Locus ID: 298487
• Cytogenetics: 5q36
• MW: 22.1 kDa