Atp2a1 (NM_058213) Rat Tagged ORF Clone

CAT#: RR207345

Atp2a1 (Myc-DDK-tagged ORF) - Rat ATPase, Ca++ transporting, cardiac muscle, fast twitch 1 (Atp2a1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Atp2a1
• Synonyms: Serca1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207345 representing NM_058213
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCCGCACACTCCAAGTCCACAGAGGAATGTTTGTCCTATTTTGGGGTGAGCGAGACCACAGGCC
  TTACCCCAGACCAAGTTAAGCGGCATCTGGAAAAATACGGCCCCAATGAGCTCCCTGCTGAGGAAGGCAA
  GTCTCTGTGGGAGTTGGTGGTAGAGCAGTTCGAAGACCTGCTGGTGCGGATTCTCCTCCTGGCTGCCTGC
  ATTTCCTTTGTGCTGGCCTGGTTTGAGGAAGGTGAGGAAACTGTCACTGCCTTTGTGGAGCCTTTTGTCA
  TTCTCTTGATCCTCATTGCCAACGCCATTGTGGGGGTTTGGCAGGAACGGAATGCAGAGAATGCCATCGA
  GGCGCTGAAGGAATATGAACCCGAGATGGGGAAGGTCTATCGGGCTGACCGAAAGTCAGTGCAAAGGATC
  AAGGCTCGGGACATCGTCCCTGGGGACATTGTGGAGGTGGCTGTTGGGGACAAAGTCCCTGCAGACATCC
  GAATCCTCTCCATCAAATCCACCACCCTCCGGGTCGACCAGTCCATCCTGACAGGAGAGTCTGTATCTGT
  CATCAAGCACACAGACCCTGTCCCTGATCCCCGAGCTGTCAACCAGGACAAGAAGAACATGCTCTTCTCG
  GGTACCAACATTGCAGCCGGCAAGGCCGTAGGCATCGTGGCCACCACTGGTGTGAGTACTGAGATTGGCA
  AGATCCGAGACCAAATGGCTGCCACTGAGCAGGACAAGACCCCGCTTCAGCAGAAGCTTGATGAGTTTGG
  GGAACAGCTGTCCAAGGTCATCTCTCTCATCTGTGTGGCTGTCTGGCTTATCAACATTGGTCACTTCAAC
  GACCCTGTCCATGGGGGCTCCTGGTTCCGGGGGGCCATTTACTACTTCAAGATTGCTGTAGCCTTGGCTG
  TGGCAGCCATCCCAGAAGGGCTTCCTGCTGTCATCACCACCTGCTTGGCCTTGGGTACCCGCCGGATGGC
  AAAGAAGAACGCCATCGTGAGGAGTTTGCCCTCTGTGGAGACCCTGGGCTGTACCTCTGTCATCTGTTCT
  GACAAAACAGGGACCCTCACCACCAACCAGATGTCAGTTTGCAAGATGTTCATCATTGACAAAGTGGATG
  GGGATATCTGCTCACTGAACGAGTTCTCCATCACTGGCTCTACTTATGCTCCTGAGGGAGAAGTTTTGAA
  GAATGATAAGCCAGTCCGAGCAGGGCAGTATGACGGGCTGGTGGAACTTGCTACCATCTGTGCCCTCTGC
  AATGACTCCTCCTTGGACTTTAATGAGACCAAAGGTGTCTATGAGAAGGTGGGCGAGGCCACCGAGACAG
  CCCTCACCACCTTGGTGGAGAAGATGAATGTGTTCAACACGGAAGTGAGAAGCCTCTCCAAAGTGGAGCG
  GGCTAATGCCTGCAACTCGGTGATCCGCCAACTAATGAAGAAGGAATTCACCCTGGAATTTTCCCGAGAC
  AGGAAGTCTATGTCTGTCTACTGCTCCCCAGCCAAGTCTTCACGGGCTGCTGTAGGCAACAAGATGTTTG
  TCAAGGGTGCCCCTGAGGGCGTCATTGACCGCTGTAACTATGTGCGTGTTGGCACAACCCGTGTGCCACT
  GACAGGCCCTGTGAAGGAGAAGATCATGTCGGTGATCAAGGAGTGGGGTACTGGTCGGGATACCCTACGG
  TGCCTGGCACTGGCCACCCGGGACACACCCCCCAAACGGGAGGAAATGGTTCTGGATGACTCAGCCAAGT
  TCATGGAGTATGAGATGGACCTGACGTTTGTTGGTGTGGTGGGCATGTTGGATCCACCCCGTAAGGAGGT
  CACAGGCTCTATCCAGCTGTGTCGTGATGCCGGGATCCGAGTGATCATGATCACTGGGGACAACAAGGGT
  ACCGCCATCGCCATCTGCCGACGAATTGGCATCTTTTCGGAGAATGAGGAAGTGGCAGACCGTGCCTACA
  CTGGCCGTGAGTTTGATGATCTGCCCCTGGCTGAGCAGCGGGAAGCCTGCAGACGCGCCTGCTGCTTCGC
  TCGTGTGGAGCCCTCACATAAGTCCAAGATTGTGGAGTACCTGCAGTCCTATGATGAGATCACAGCCATG
  ACAGGGGATGGTGTCAATGATGCCCCTGCTCTGAAGAAGGCGGAGATTGGCATTGCCATGGGATCTGGTA
  CTGCTGTGGCTAAGACTGCCTCCGAGATGGTACTGGCTGATGATAACTTCTCCACCATTGTGGCTGCCGT
  GGAGGAAGGCCGTGCCATCTACAACAACATGAAGCAGTTCATCCGCTACCTCATTTCCTCCAATGTGGGC
  GAGGTGGTCTGTATCTTCTTGACAGCAGCCTTGGGGCTGCCTGAGGCCTTGATCCCTGTGCAGCTGCTAT
  GGGTGAACTTGGTGACTGATGGGCTCCCTGCTACTGCCCTGGGGTTCAACCCACCTGACCTGGACATCAT
  GGACCGTCCCCCCAGGAGTCCCAAGGAGCCTCTTATCAGTGGCTGGCTCTTTTTCCGCTACATGGCAATT
  GGGGGCTACGTGGGTGCAGCCACTGTGGGAGCAGCTGCCTGGTGGTTCTTGTATGCAGAGGATGGGCCTC
  ATGTCAGCTACCATCAACTGACTCATTTCATGCAGTGCACTGAGCATAACCCTGAATTTGATGGCCTGGA
  CTGCGAGGTCTTTGAAGCCCCCGAGCCCATGACCATGGCCTTGTCTGTGTTGGTGACCATCGAGATGTGC
  AACGCCCTCAACAGCCTGTCTGAGAACCAGTCCCTACTGCGGATGCCGCCCTGGGTGAACATCTGGCTTC
  TCGGTTCCATCTGCCTGTCCATGTCCCTCCACTTCCTCATCCTCTATGTTGACCCCCTGCCGATGATCTT
  CAAGCTCCGGGCCCTGGACTTTACCCAGTGGCTCATGGTCCTCAAGATCTCACTTCCAGTCATCGGGCTA
  GATGAGCTTCTCAAGTTCATTGCTCGGAACTATCTGGAGGGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207345 representing NM_058213
  Red=Cloning site Green=Tags(s)
  
  MEAAHSKSTEECLSYFGVSETTGLTPDQVKRHLEKYGPNELPAEEGKSLWELVVEQFEDLLVRILLLAAC
  ISFVLAWFEEGEETVTAFVEPFVILLILIANAIVGVWQERNAENAIEALKEYEPEMGKVYRADRKSVQRI
  KARDIVPGDIVEVAVGDKVPADIRILSIKSTTLRVDQSILTGESVSVIKHTDPVPDPRAVNQDKKNMLFS
  GTNIAAGKAVGIVATTGVSTEIGKIRDQMAATEQDKTPLQQKLDEFGEQLSKVISLICVAVWLINIGHFN
  DPVHGGSWFRGAIYYFKIAVALAVAAIPEGLPAVITTCLALGTRRMAKKNAIVRSLPSVETLGCTSVICS
  DKTGTLTTNQMSVCKMFIIDKVDGDICSLNEFSITGSTYAPEGEVLKNDKPVRAGQYDGLVELATICALC
  NDSSLDFNETKGVYEKVGEATETALTTLVEKMNVFNTEVRSLSKVERANACNSVIRQLMKKEFTLEFSRD
  RKSMSVYCSPAKSSRAAVGNKMFVKGAPEGVIDRCNYVRVGTTRVPLTGPVKEKIMSVIKEWGTGRDTLR
  CLALATRDTPPKREEMVLDDSAKFMEYEMDLTFVGVVGMLDPPRKEVTGSIQLCRDAGIRVIMITGDNKG
  TAIAICRRIGIFSENEEVADRAYTGREFDDLPLAEQREACRRACCFARVEPSHKSKIVEYLQSYDEITAM
  TGDGVNDAPALKKAEIGIAMGSGTAVAKTASEMVLADDNFSTIVAAVEEGRAIYNNMKQFIRYLISSNVG
  EVVCIFLTAALGLPEALIPVQLLWVNLVTDGLPATALGFNPPDLDIMDRPPRSPKEPLISGWLFFRYMAI
  GGYVGAATVGAAAWWFLYAEDGPHVSYHQLTHFMQCTEHNPEFDGLDCEVFEAPEPMTMALSVLVTIEMC
  NALNSLSENQSLLRMPPWVNIWLLGSICLSMSLHFLILYVDPLPMIFKLRALDFTQWLMVLKISLPVIGL
  DELLKFIARNYLEG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_058213
• ORF Size: 2982 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_058213.1, NP_478120.1
• RefSeq Size: 3457
• RefSeq ORF: 2985
• Locus ID: 116601
• MW: 109.4 kDa
• Gene Summary: catalyzes ATP hydrolysis and calcium ion transport [RGD, Feb 2006]