Phkg1 (NM_031573) Rat Tagged ORF Clone

CAT#: RR207349

Phkg1 (Myc-DDK-tagged ORF) - Rat phosphorylase kinase gamma 1 (Phkg1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Phkg1
• Synonyms: Phkg; PHKIN01
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207349 representing NM_031573
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCCGGGATGAGGCCCTCCCTGACTCTCATTCTGCACAGAATTTCTATGAGAACTACGAGCCCAAGG
  AGATCCTGGGCAGGGGAGTCAGCAGCGTGGTCAGGAGATGCATTCACAAACCTACGTGCCAAGAATACGC
  AGTAAAAATCATCGACATCACCGGCGGAGGAAGCTTTAGCTCCGAGGAGGTACAGGAGCTTCGGGAAGCC
  ACCCTGAAGGAGGTGGACATTCTGCAGAAGGTCTCAGGACACCCCAACATCATACAGCTGAAGGACACTT
  ACGAGACCAACACTTTCTTCTTCTTGGTATTTGATCTGATGAAGAGAGGGGAACTCTTTGACTATCTCAC
  AGAGAAGGTCACCTTGACTGAGAAGGAAACCAGAAAGATCATGCGGGCCCTGCTGGAGGTGGTCTGCACC
  TTGCACAAACTCAACATTGTCCATCGGGACCTGAAGCCGGAGAACATCCTTCTGGATGACAACATGAACA
  TCAAGCTCACTGACTTCGGGTTTTCCTGCCAGCTGCAGCCAGGAGAGAAGCTCCGAGAGGTTTGTGGGAC
  TCCCAGTTATCTGGCCCCCGAAATCATCCAGTGCTCCATGGACGAAGGCCATCCTGGCTATGGAAAGGAG
  GTGGACATGTGGAGCACAGGAGTCATCATGTATACGCTGCTGGCTGGCTCCCCGCCCTTCTGGCACCGGA
  AGCAAATGCTAATGTTGAGGATGATCATGGATGGCAAATACCAGTTCGGCTCACCAGAGTGGGATGACTA
  CTCAGATACCGTGAAAGACTTGGTATCTCGCTTCTTGGTGGTGCAACCCCAGGACCGCTGCTCGGCGGAA
  GAGGCCTTGGCGCATCCTTTCTTCCAGGAGTACGTGGTGGAAGAGGTGCGGCACTTCAGCCCTCGAGGGA
  AGTTCAAGGTCATCTGTCTAACTGTGCTGGCCTCGGTAAGGATCTACTACCAGTACCGTCGGGTGAAGCC
  GGTAACCAGGGAGATCGTCATCCGAGACCCCTACGCCCTACGGCCATTGCGCAGACTCATCGACGCCTAT
  GCTTTCCGCATCTACGGCCACTGGGTGAAGAAAGGGCAACAGCAGAACAGAGCTGCCCTCTTCGAGAACA
  CGCCCAAGGCAGTGCTCCTCTCCTTGGCTGAGGAGGAGGACTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207349 representing NM_031573
  Red=Cloning site Green=Tags(s)
  
  MTRDEALPDSHSAQNFYENYEPKEILGRGVSSVVRRCIHKPTCQEYAVKIIDITGGGSFSSEEVQELREA
  TLKEVDILQKVSGHPNIIQLKDTYETNTFFFLVFDLMKRGELFDYLTEKVTLTEKETRKIMRALLEVVCT
  LHKLNIVHRDLKPENILLDDNMNIKLTDFGFSCQLQPGEKLREVCGTPSYLAPEIIQCSMDEGHPGYGKE
  VDMWSTGVIMYTLLAGSPPFWHRKQMLMLRMIMDGKYQFGSPEWDDYSDTVKDLVSRFLVVQPQDRCSAE
  EALAHPFFQEYVVEEVRHFSPRGKFKVICLTVLASVRIYYQYRRVKPVTREIVIRDPYALRPLRRLIDAY
  AFRIYGHWVKKGQQQNRAALFENTPKAVLLSLAEEEDF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031573
• ORF Size: 1164 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_031573.1, NP_113761.1
• RefSeq Size: 1388
• RefSeq ORF: 1167
• Locus ID: 29353
• MW: 45 kDa
• Gene Summary: catalytic subunit of phosphorylase kinase complex; plays a role in glycogen metabolism [RGD, Feb 2006]