Tk1 (NM_052800) Rat Tagged ORF Clone

CAT#: RR207356

Tk1 (Myc-DDK-tagged ORF) - Rat thymidine kinase 1, soluble (Tk1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tk1
• Synonyms: Tk
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207356 representing NM_052800
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTACATCAACCTGCCCACCGTGCTGCCCATCTCCCCCAGCAAGACCCGGGGGCAGATCCAGGTGA
  TTCTCGGGCCCATGTTCTCAGGGAAAAGCACGGAGCTGATGAGGAGAGTAAGGCGCTTCCAGATCGCCCA
  GTACAAGTGCCTGGTCATCAAGTATGCCAAAGACACGCGCTATAGCAACAGCTTCTCCACACATGACCGG
  AACACCATGGATGCACTGCCGGCCTGCATGCTCAAGGATGTGGCCCAGGAGGCCTTGGGTGTGGCTGTCA
  TTGGCATCGATGAAGGGCAGTTTTTCCCTGACATTGTGGATTTCTGTGAAACGATGGCCAATACAGGCAA
  GACAGTGATTGTGGCGGCGCTGGACGGAACCTTCCAGAGGAAGGCTTTCGGCAGCATCTTGAACCTGGTG
  CCCTTGGCCGAGAGTGTGGTGAAGCTCACCGCTGTGTGCATGGAGTGCTTTCGAGAAGCTTCCTACACCA
  AGAGGCTGGGCCTGGAGAAAGAGGTGGAGGTGATTGGCGGAGCGGACAAGTACCACTCTGTTTGCCGCCT
  GTGCTACTTTAAGAAGTCTTCAGCCCAGACTGCAGACAACAAAGAGAACTACTCAGTGCTGGGGCAGCCT
  ATAGAGATCCCGGCTGTCAGGAAGCTCTTTGCCCCTCAGCAAATCCTACAGTGTAACTCTGCCAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207356 representing NM_052800
  Red=Cloning site Green=Tags(s)
  
  MSYINLPTVLPISPSKTRGQIQVILGPMFSGKSTELMRRVRRFQIAQYKCLVIKYAKDTRYSNSFSTHDR
  NTMDALPACMLKDVAQEALGVAVIGIDEGQFFPDIVDFCETMANTGKTVIVAALDGTFQRKAFGSILNLV
  PLAESVVKLTAVCMECFREASYTKRLGLEKEVEVIGGADKYHSVCRLCYFKKSSAQTADNKENYSVLGQP
  IEIPAVRKLFAPQQILQCNSAN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_052800
• ORF Size: 696 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_052800.1, NP_434687.1
• RefSeq Size: 2263
• RefSeq ORF: 699
• Locus ID: 24834
• MW: 26.2 kDa
• Gene Summary: catalyzes the transfer of the terminal phosphate of ATP to thymidine to form thymidine 5'-phosphate (dTMP) [RGD, Feb 2006]