Top3b (NM_001105861) Rat Tagged ORF Clone

CAT#: RR207379

Top3b (Myc-DDK-tagged ORF) - Rat topoisomerase (DNA) III beta (Top3b), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Top3b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207379 representing NM_001105861
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGACGGTGCTCATGGTAGCAGAAAAGCCATCCCTGGCACAATCCATTGCCAAAATCCTCTCCCGAG
  GAAACATGTCCTCACACAAAGGGCTAAATGGGGCGTGTTCAGTGCACAAATACACAGGAACCTTTGCTGG
  CCAGCCCGTCCACTTCAAGATGACATCTGTCTGTGGTCATGTGATGACACTGGATTTTCTGGGAAAGTAC
  AACAAGTGGGATAAGGTAGATCCTGCAGAGTTATTCAGCCAAGCTCCAACAGAGAAAAAGGAAGCAAACC
  CCAAGCTGAACATGGTCAAGTTCCTGCAGGTGGAGGGCAGAGGCTGTGACTACGTCGTGCTGTGGCTGGA
  CTGTGACAAGGAAGGCGAGAATATTTGCTTTGAGGTCCTTGATGCCGTTCTGCCTGTCCTGAATAATGCC
  CACAATGGTGAGAAGACAGTGTTCCGGGCCAGGTTCAGTTCCATCACAGACACGGACATCTGCAATGCCA
  TGACCCGCCTGACTGAGCCAGACCACAATGAGGCACTCTCGGTGGATGCCCGCCAGGAGCTAGACCTGCG
  CATCGGCTGTGCGTTCACTAGGTTTCAAACAAAGTATTTCCAGGGAAAATATGGCGACTTAGACAGCTCT
  CTCATCTCCTTTGGGCCTTGTCAGACTCCAACCCTGGGATTCTGTGTAGAAAGACATGATAAAATCCAGT
  CCTTCAAACCAGAGACCTACTGGGTACTTCAGGCCAAGGTTCATACTGATAAAGAGGAGTCACTCCTTTT
  GGACTGGGACCGAGTTAGAGTTTTCGACCGGGAGATTGCACAGATGTTTTTAAACATGACAAAGCTAGAA
  AAAGAAGCTTGGGTGGAGGCTACAAGCAAGAAAGAAAAAGCCAAGCAGAGACCACTTGCTCTGAACACTG
  TGGAGATGTTGCGTGTGGCCAGTTCAGCTCTGGGTATGGGACCACAGCATGCCATGCAAGTAGCTGAGAG
  ATTATACACACAGGGCTACATCAGCTACCCACGGACAGAAACCACACACTATCCCGAGAACTTTGACCTG
  AAGGGCTCTTTACGACAACAGGCCAATCACCCATACTGGGCAGACTCGGTAAAGCAGTTGTTAGCAGAAG
  GCATCAACCGCCCTCGGAAAGGTCACGATGCTGGTGACCATCCTCCGATCACACCAATGAAGTCAGCTAC
  AGAGGCTGAATTAGGAGGTGACGCTTGGCGACTCTACGAGTACATCACCAGACACTTCATCGCCACAGTG
  AGCCATGACTGCAAGTATCTCCAGAGCACCATCTCCTTCAGGATCGGGCCCGAGCACTTCACATGCATGG
  GAAAGACAGTTATTTCCCCAGGCTTCACAGAGATCATGCCTTGGCAGAGCGTGCCCCTGGAAGAGAGTCT
  GCCCACATGCCAGAAGGGCGATACCTTCACTGTGGGTGAGGTGAAGATGCTAGAGAAGCAGACGAGCCCC
  CCGGACTATCTGACTGAGGCTGAGCTCATCACCCTCATGGAGAAGCATGGCATCGGGACGGATGCCAGTA
  TCCCTGTGCACATCAACAACATCTGCCAGCGCAACTACGTCACAGTGGAGAACGGCCGCCGGCTCAAGCC
  CACCAACCTCGGCATAGTTCTGGTGCACGGCTACCACAAGATTGATGCAGAGCTGGTGCTCCCCACCATC
  CGCAGCGCAGTGGAGAAGCAGCTGAACCTTATTGCCCAGGGCAAAGCCAACTACCACCAGGTGCTGGGCC
  ACACCCTGGATATCTTCAAGAGAAAGTTCCACTACTTTGTTGATTCTATTGCTGGCATGGACGAGTTAAT
  GGAGGTATCCTTTTCACCCCTGGCTGCCACTGGCAAGCCCCTCTCGCGCTGTGGGAAGTGCCATCGATTT
  ATGAAGTATATCCAGGCCAAGCCAAGCCGCCTGCACTGCTCCCACTGCGATGAGACCTACACCCTCCCCC
  AGAATGGTACCATCAAGCTCTACAAGGAGCTGCGGTGCCCATTGGATGACTTTGAACTGGTCTTGTGGTC
  CTCAGGCTCCCGGGGCAAGAGCTACCCCCTCTGTCCCTACTGCTATAACCACCCACCCTTCCGAGACATG
  AAGAAAGGCATGGGCTGCAATGAGTGCACACACCCTACGTGCCAGCACTCACTGAGTATGCTGGGCATTG
  GCCAGTGCGTGGAGTGCGAGAATGGAGTCCTGGTTCTGGACCCCACCTCCGGTCCCAAGTGGAAGGTGGC
  CTGCAATACATGCAGCGTAGTGGCACACTGCTTTGACAATGCCCACCGAGTACGGGTGTCTGCTGACACC
  TGCAACACCTGCGAGGCTGCCTTGCTCGATGTAGACTTCAACAAGGCCAAGTCTCCCCTTCCTGGCAACG
  AGACACAGCACACAGGCTGCATCTTCTGTGACACCGTCTTCCAGGAGCTGGTGGAGCTGAAGCATGCAGC
  CTCCTGCCACCCCATGCACCGAGGTGGTCCTGGAAGGAGGCAGGGTCGTGGGCGGGCCCGGGGCCGGAGA
  CCTCCAGGAAAGCCCAACCCCAGGCGGCCAAAAGACAAGATGTCAGCCCTGGCTGCTTACTTTGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207379 representing NM_001105861
  Red=Cloning site Green=Tags(s)
  
  MKTVLMVAEKPSLAQSIAKILSRGNMSSHKGLNGACSVHKYTGTFAGQPVHFKMTSVCGHVMTLDFLGKY
  NKWDKVDPAELFSQAPTEKKEANPKLNMVKFLQVEGRGCDYVVLWLDCDKEGENICFEVLDAVLPVLNNA
  HNGEKTVFRARFSSITDTDICNAMTRLTEPDHNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSS
  LISFGPCQTPTLGFCVERHDKIQSFKPETYWVLQAKVHTDKEESLLLDWDRVRVFDREIAQMFLNMTKLE
  KEAWVEATSKKEKAKQRPLALNTVEMLRVASSALGMGPQHAMQVAERLYTQGYISYPRTETTHYPENFDL
  KGSLRQQANHPYWADSVKQLLAEGINRPRKGHDAGDHPPITPMKSATEAELGGDAWRLYEYITRHFIATV
  SHDCKYLQSTISFRIGPEHFTCMGKTVISPGFTEIMPWQSVPLEESLPTCQKGDTFTVGEVKMLEKQTSP
  PDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTVENGRRLKPTNLGIVLVHGYHKIDAELVLPTI
  RSAVEKQLNLIAQGKANYHQVLGHTLDIFKRKFHYFVDSIAGMDELMEVSFSPLAATGKPLSRCGKCHRF
  MKYIQAKPSRLHCSHCDETYTLPQNGTIKLYKELRCPLDDFELVLWSSGSRGKSYPLCPYCYNHPPFRDM
  KKGMGCNECTHPTCQHSLSMLGIGQCVECENGVLVLDPTSGPKWKVACNTCSVVAHCFDNAHRVRVSADT
  CNTCEAALLDVDFNKAKSPLPGNETQHTGCIFCDTVFQELVELKHAASCHPMHRGGPGRRQGRGRARGRR
  PPGKPNPRRPKDKMSALAAYFV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001105861
• ORF Size: 2586 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001105861.1, NP_001099331.1
• RefSeq Size: 2966
• RefSeq ORF: 2589
• Locus ID: 287930
• MW: 96.9 kDa