Mlh1 (NM_031053) Rat Tagged ORF Clone

CAT#: RR207442

Mlh1 (Myc-DDK-tagged ORF) - Rat mutL homolog 1 (E. coli) (Mlh1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mlh1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207442 representing NM_031053
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCATTTGTAGCAGGAGTTATTCGGCGCCTGGACGAGACGGTAGTGAATCGCATAGCGGCGGGAGAAG
  TTATCCAGCGGCCGGCCAATGCTATCAAAGAGATGACTGAAAACTGTTTAGATGCAAAATCTACAAATAT
  TCAAGTGATTGTTAGAGAAGGAGGCCTGAAGCTAATTCAGATCCAAGACAATGGCACTGGAATCAGGAAG
  GAAGATTTGGATATTGTGTGTGAGAGGTTCACTACAAGTAAACTGCAGACCTTTGAGGATTTAGCTATGA
  TTTCCACCTATGGGTTTCGCGGAGAGGCTTTGGCAAGCATAAGCCATGTGGCCCATGTCACTATTACAAC
  CAAAACAGCTGATGGGAAATGTGCATACAGAGCAAGTTACTCAGATGGAAAGCTACAAGCCCCTCCTAAA
  CCGTGTGCAGGCAACCAGGGTACCCTGATCACGGTGGAAGACCTTTTTTACAACATAATCACAAGGAAGA
  AAGCTTTAAAAAATCCCAGTGAAGAGTACGGGAAGATTTTGGAAGTTGTTGGCAGGTACTCAATACACAA
  TTCAGGAATTAGTTTCTCAGTTAAAAAACAAGGCGAGACAGTCTCTGATGTCAGAACACTACCCAATGCC
  ACGACCGTAGACAACATTCGCTCCATCTTTGGAAATGCTGTTAGTCGAGAACTGATCGAAGTTGGGTGTG
  AGGATAAAACCCTAGCTTTCAAAATGAATGGCTATATCTCGAATGCAAACTACTCAGTGAAGAAGTGCAT
  TTTCCTACTCTTCATCAACCACCGGCTGGTGGAATCGGCTGCCCTGAAGAAAGCCATAGAAGCTGTATAT
  GCAGCATATTTACCCAAAAACACGCACCCATTCCTGTACCTCATTTTGGAAATCAGTCCCCAGAACGTGG
  ATGTCAATGTGCACCCCACCAAGCATGAAGTTCACTTTCTCCATGAGGAGAGTATTCTGGAGCGTGTGCA
  GCAGCACATCGAGAGCAAGCTATTGGGCTCCAATTCATCCAGGATGTATTTTACCCAGACCTTGCTTCCA
  GGACTTGCTGGGCCCTCTGGGGAAGCCGTTAAATCCACGACAGGCATAGCTTCCTCATCTACTAGTGGAA
  GTGGCGACAAGGTTCACGCTTACCAAATGGTCCGTACAGACTCTCGGGACCAGAAGCTCGATGCCTTTAT
  GCAGCCTGTAAGCAGGCGTCTGCCCAGCCAGCCCCAGGACCCTGTCCCTGGGAACAGGACAGAGGGCTCT
  CCTGAAAAAGCCATGCAGAAGGATCAGGAGATATCTGAGCTCCCGGCCCCCATGGAAGCAGCTGCTGACA
  GTGCGAGTTTGGAGAGAGAATCAGTGATTGGGGCTTCGGAAGTGGTGGCACCCCAGCGGCACCCCTCCAG
  TCCAGGCAGCTCCAGAAAGAGACATCCGGAGGACTCCGATGTGGAAATGATGGAAAATGATTCCCGGAAG
  GAAATGACAGCTGCTTGCTACCCCCGGAGGAGGATCATTAACCTCACCAGTGTTTTGAGTCTCCAGGAAG
  AAATTAACGACCGGGGTCATGAGACTCTCCGGGAGATGCTCCGTAACCATACCTTTGTGGGCTGTGTGAA
  TCCTCAGTGGGCCTTGGCACAGCACCAGACCAAGCTCTACCTCCTCAACACTACCAAGCTCAGTGAAGAA
  CTCTTCTACCAGATACTCATTTACGATTTTGCCAACTTCGGTGTTCTCAGGTTACCGGAACCAGCACCAC
  TCTTTGACTTCGCCATGCTGGCCTTAGATAGTCCTGAAAGTGGCTGGACAGAGGAAGATGGCCCAAAGGA
  AGGACTTGCAGAATACATTGTTGAGTTTTTGAAGAAGAAAGCCAAGATGCTTGCAGACTATTTCTCTGTG
  GAAATTGATGAGGAAGGGAACCTGATTGGATTACCTCTCCTGATTGATAGCTATGTGCCGCCTTTGGAGG
  GACTGCCTATCTTCATTCTTCGACTGGCCACTGAGGTGAACTGGGATGAAGAGGAGTGTTTCGAAAGTCT
  CAGTAAAGAATGTGCTGTGTTTTACTCCATTCGGAAGCAATACATACTGGAGGAGTCAGCCCTCTCAGGC
  CAGCAGAGTGACATGCCTGGCTCCCCTTCAAAGCCCTGGAAGTGGACTGTGGAACACATTATCTATAAAG
  CCTTCCGCTCACACCTTCTGCCTCCGAAGCATTTCACAGAAGATGGCAATGTCCTGCAGCTCGCCAACCT
  GCCAGATCTGTGCAAAGTCTTCGAACGCTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207442 representing NM_031053
  Red=Cloning site Green=Tags(s)
  
  MSFVAGVIRRLDETVVNRIAAGEVIQRPANAIKEMTENCLDAKSTNIQVIVREGGLKLIQIQDNGTGIRK
  EDLDIVCERFTTSKLQTFEDLAMISTYGFRGEALASISHVAHVTITTKTADGKCAYRASYSDGKLQAPPK
  PCAGNQGTLITVEDLFYNIITRKKALKNPSEEYGKILEVVGRYSIHNSGISFSVKKQGETVSDVRTLPNA
  TTVDNIRSIFGNAVSRELIEVGCEDKTLAFKMNGYISNANYSVKKCIFLLFINHRLVESAALKKAIEAVY
  AAYLPKNTHPFLYLILEISPQNVDVNVHPTKHEVHFLHEESILERVQQHIESKLLGSNSSRMYFTQTLLP
  GLAGPSGEAVKSTTGIASSSTSGSGDKVHAYQMVRTDSRDQKLDAFMQPVSRRLPSQPQDPVPGNRTEGS
  PEKAMQKDQEISELPAPMEAAADSASLERESVIGASEVVAPQRHPSSPGSSRKRHPEDSDVEMMENDSRK
  EMTAACYPRRRIINLTSVLSLQEEINDRGHETLREMLRNHTFVGCVNPQWALAQHQTKLYLLNTTKLSEE
  LFYQILIYDFANFGVLRLPEPAPLFDFAMLALDSPESGWTEEDGPKEGLAEYIVEFLKKKAKMLADYFSV
  EIDEEGNLIGLPLLIDSYVPPLEGLPIFILRLATEVNWDEEECFESLSKECAVFYSIRKQYILEESALSG
  QQSDMPGSPSKPWKWTVEHIIYKAFRSHLLPPKHFTEDGNVLQLANLPDLCKVFERC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031053
• ORF Size: 2271 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_031053.1, NP_112315.1
• RefSeq Size: 2274
• RefSeq ORF: 2274
• Locus ID: 81685
• MW: 84.5 kDa
• Gene Summary: may be involved in DNA mismatch repair [RGD, Feb 2006]