Atg4a (NM_001126298) Rat Tagged ORF Clone

CAT#: RR207459

Atg4al1 (Myc-DDK-tagged ORF) - Rat similar to Cysteine protease ATG4A (Autophagy-related protein 4 homolog A) (Autophagin-2) (Autophagy-related cysteine endopeptidase 2) (AUT-like 2 cystein, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Atg4a
• Synonyms: Agt4a; Atg4al1; RGD1588268
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207459 representing NM_001126298
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTCAGTTATGTCCAAGTATGAAAACCAGATTATTATTTTCACTGAATACCTAGAAGAATTCCCAG
  ATACAGATGAGCTAGTATGGATCCTGGGGAAGCCACATCTCCTTAAGACAGAAAAATCTAAGCTGTTGTC
  TGATATAAGTGCTCGTCTATGGTTTACATACAGAAGGAAATTTTCACCGATTGGGGGAACAGGCCCTTCG
  TCGGATGCTGGCTGGGGATGTATGCTGCGCTGTGGGCAGATGATGCTGGCTCAAGCCCTCATCTGTAGAC
  ACTTGGGAAGGGATTGGAACTGGGAGAGACAAAAAGAACAACCCAAAGAATACCAAAGGATACTGCAGTG
  TTTCCTAGATAGAAAAGACTGTTGCTATTCAATCCATCAGATGGCACAAATGGGTGTAGGAGAAGGGAAA
  TCAATTGGCGAATGGTTTGGACCAAATACAGTTGCACAGGTGATAAAAAAACTTGCTTTATTTGATGAAT
  GGAATTCCTTGGCTGTTTATGTTTCAATGGATAACACAGTGGTCATTGAAGATATCAAGAAAATGTGCTG
  TGTTCTTCCTGTGGGTGCTGCTGACACAGCTGGTGAGAGCCCTCCTGATTCTCTGATTGCTTCCAGTCAG
  AGTAAGGGCACCTCTGCCCCGTGCCTAGCCTGGAAACCCCTGCTGCTCATTGTGCCCCTTCGCCTGGGCA
  TAAACCAAATCAACCCTGTGTATATTGAAGCATTCAAAGAGTGTTTTAAGATGCCACAGTCTTTAGGGGC
  TTTAGGAGGAAAGCCAAATAACGCCTATTATTTCATAGGATCCTTAGGTGACGAGCTCATTTTCTTGGAT
  CCTCACACAACCCAGACCTTCGTTGATACTGAAGAGAGTGGACTAGTAGACGACCACACTTTCCATTGCC
  TGCAGTCTCCACAGCGGATGAGTATCCTGAACTTGGATCCTTCTGTGGCCTTGGTGGGTCAAGGAGCTTT
  TATGGGATTTTTCTGCAAAGAAGAGAAAGACTTTGATAATTGGTGTAGTCTTGTTCAGAAGGAAATTCTA
  AAGGAGAATTTGAGGATGTTTGAATTAGTTCAGAAACATCCATCACACTGGCCTCCCTTTGTACCTCCAG
  CCAAGCCAGAAGTGACAACCACAGGGGCAGAATTCATAGACTCTACCGAACAACTGGAGGACTTCGACCT
  GGAGGAAGATTTTGAGATTCTGAGTGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207459 representing NM_001126298
  Red=Cloning site Green=Tags(s)
  
  MESVMSKYENQIIIFTEYLEEFPDTDELVWILGKPHLLKTEKSKLLSDISARLWFTYRRKFSPIGGTGPS
  SDAGWGCMLRCGQMMLAQALICRHLGRDWNWERQKEQPKEYQRILQCFLDRKDCCYSIHQMAQMGVGEGK
  SIGEWFGPNTVAQVIKKLALFDEWNSLAVYVSMDNTVVIEDIKKMCCVLPVGAADTAGESPPDSLIASSQ
  SKGTSAPCLAWKPLLLIVPLRLGINQINPVYIEAFKECFKMPQSLGALGGKPNNAYYFIGSLGDELIFLD
  PHTTQTFVDTEESGLVDDHTFHCLQSPQRMSILNLDPSVALVGQGAFMGFFCKEEKDFDNWCSLVQKEIL
  KENLRMFELVQKHPSHWPPFVPPAKPEVTTTGAEFIDSTEQLEDFDLEEDFEILSV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001126298
• ORF Size: 1218 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001126298.1, NP_001119770.1
• RefSeq Size: 2171
• RefSeq ORF: 1221
• Locus ID: 678769
• MW: 45.9 kDa