Hk3 (NM_022179) Rat Tagged ORF Clone

CAT#: RR207490

Hk3 (Myc-DDK-tagged ORF) - Rat hexokinase 3 (white cell) (Hk3), nuclear gene encoding mitochondrial protein, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hk3
• Synonyms: RNU73859
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207490 representing NM_022179
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCCATTGAGCCTTCTGGTCTGCACCCGGGAGAAAGAGACTCAAGCTGCCCCCAGGAGGGCATTC
  CAAGGCCCTCAGGTAGCTTAGAACTGGCACAGGAATACTTGCAACAATTCAAGGTGACCATGACACAGCT
  GCAGCAGATCCAAGCCAGTCTTCTGTGTTCCATGGAGCAGGCGCTGAAGGGACAGGACAGTCCCGCTCCT
  TCTGTCCGGATGTTGCCCACATACGTGAGGTCCACACCACATGGCACCGAGCAAGGAGACTTCCTGGTGC
  TGGAGCTGGGGGCCACAGGAGCCTCACTACGTGTGTTGTGGGTAACACTGACGGGCACCAAGGAACACAG
  CGTGGAGACCAGGAGCCAGGAGTTTGTGATCCCTCAAGAGGTGATCCTAGGTGCTGGCCAGCAGCTCTTT
  GACTTTGCTGCCCGCTGCCTCTCTGAATTCCTGGATGCATACCCCGTGGAGAATCAGGGTCTGAAGCTTG
  GGTTTAATTTCTCTTTTCCTTGTCACCAGACAGGCTTGGACAAGAGCACCCTCATTTCCTGGACAAAAGG
  TTTTAGGTGCAGTGGTGTGGAAGGCCAGGATGTGGTCCAGTTGCTAAGGGATGCCATTCAGAGGCAGGGG
  ACCTACAATATTGATGTGGTAGCCATGGTGAATGACACAGTGGGTACCATGATGGGCTGTGAGCTGGGCA
  CCAGGCCATGTGAAGTCGGGCTTATTGTAGACACTGGTACCAATGCCTGTTATATGGAGGAAGCGAGGCA
  CGTGGCAGCTCTGGATGAGGACCGCGGCCGTACCTGTGTCAGCATCGAGTGGGGCTCCTTCTATGACGAA
  GAGGCCCTAGGGCCAGTACTGACCACCTTCGACGATGCCCTGGACCACGAGTCCCTGGTTCCTGGTGCTC
  AGAGGTTTGAGAAGATGATTGGTGGCCTTTACTTGGGTGAGCTGGTAAGGCTGGTGCTGGTCCACTTGTC
  CCAGCATGGGGTCCTCTTTGGTGGCTGCGCCTCTCCTGCGTTGCTGAGTCAAAACAGCATCCTCCTGGAA
  CATGTGGCCAAAATGGAGGACCCTGCCACTGGGATAGCCCACGTCCACACAGTCCTGCAGGGCTTGGGTC
  TGAGCCCTCAGGCCTCAGATGCTGAGCTCGTGCAGCGCGTGTGCATGGCTGTGTGCACGCGAGCTGCCCA
  GCTCTGTGCCTCTGCCCTGGCTGCAGTCCTATCCCGCCTCCAGCACAGCAGGGAGCAGCAGACACTGCAC
  GTGGCCGTGGCCACTGGAGGGCGAGTGTTCGAATGGCACCCCAGGTTCCTCTGCATCCTAAAGGAGACGG
  TAATGCTCTTGGCCCCAGAGTGTGATGTCTCCTTCATCCCCTCTGTGGATGGTGGTGGCCGGGGTGTGGC
  AATGGTGACTGCTGTGGCAGCCCGCCTGGCTACCCACAGGCGCATCCTGGAAGAGACCCTGGCACCATTT
  CAGCTGAGCTTGGAGCAGCTGACAGCGGTGCAGGCACAAATGCGGGAAGCCATGATCAGGGGGCTTCAAG
  GAGAGAGCTCCTCCCTCCGCATGCTGCCCACTTACGTCCGAGCAACGCCCGATGGCAGCGAACGAGGTGA
  CTTCCTGGCTTTGGACCTAGGGGGCACCAACTTCCGTGTCCTGTTGGTACGCGTGGCCGAGGGCAGTGTT
  CAGATCACCAACCAGGTCTACTCTATTCCTGAGTATGTAGCCCAGGGCTCTGGACAGAAGCTCTTTGATC
  ATATTGTGGACTGCATCGTGGACTTCCAGAAGAGGCAAGGCCTTAGCGGACAGAGCCTACCCCTGGGTTT
  CACCTTCTCTTTTCCTTGCAAGCAGCTTGGCCTGGACCAGGGCATCCTCCTCAACTGGACTAAGGGGTTC
  AATGCATCAGGCTGCGAGGGCCAAGATGTTGTGTATTTATTACGGGAAGCCATTAGGCGCAGACAGGCAG
  TGGAGCTGAATGTGGTTGCCATTGTCAATGACACGGTGGGGACCATGATGTCCTGTGGCTATGATGATCC
  CTGTTGTGAGATGGGCCTCATTGTCGGAACCGGTACCAACGCCTGCTATATGGAAGAACTCCGGAATGTG
  GCGAGTGTGCCCGGGGACTCAGGCCACATGTGTATCAACATGGAGTGGGGTGCCTTTGGGGATGACGGCT
  CACTGAGCATGCTCGGCACCTGCTTTGATGCTAGCGTGGACCAGGCATCCATCAACCCAGGCAAACAGAG
  GTTTGAGAAAATGATCAGCGGAATGTACCTGGGGGAGATCGTCCGCCATATCCTCCTGCACTTAACCAGT
  CTTGGAGTTCTCTTCCGGGGCCAGAAGACGCAATGCCTTCAGACCAGGGACATCTTTAAGACCAAGTTTC
  TCTCCGAGATTGAGAGCGACAGCCTGGCCCTGCGTCAGGTCCGAGCCATCCTGGAGGACCTGGGGCTGAC
  TCTGACGTCTGATGATGCCTTGATGGTCCTAGAGGTGTGCCAGGCTGTGTCCCGCAGGGCCGCCCAACTC
  TGCGGGGCAGGTGTGGCTGCAGTGGTGGAAAAGATACGGGAGAACCGGGGCCTGCAGGAGCTGACAGTGT
  CTGTGGGAGTGGATGGGACGCTCTACAAGCTACATCCCCACTTCTCCAGGCTGGTGTCAGTGACAGTTCG
  GAAGCTAGCCCCTCAGTGCACAGTCACCTTTTTGCAATCGGAGGATGGGTCTGGGAAAGGGGCAGCGTTG
  GTCACTCGTGTCGCTTGCCGCCTGACCCAGATGGCCTGCGTT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207490 representing NM_022179
  Red=Cloning site Green=Tags(s)
  
  MAAIEPSGLHPGERDSSCPQEGIPRPSGSLELAQEYLQQFKVTMTQLQQIQASLLCSMEQALKGQDSPAP
  SVRMLPTYVRSTPHGTEQGDFLVLELGATGASLRVLWVTLTGTKEHSVETRSQEFVIPQEVILGAGQQLF
  DFAARCLSEFLDAYPVENQGLKLGFNFSFPCHQTGLDKSTLISWTKGFRCSGVEGQDVVQLLRDAIQRQG
  TYNIDVVAMVNDTVGTMMGCELGTRPCEVGLIVDTGTNACYMEEARHVAALDEDRGRTCVSIEWGSFYDE
  EALGPVLTTFDDALDHESLVPGAQRFEKMIGGLYLGELVRLVLVHLSQHGVLFGGCASPALLSQNSILLE
  HVAKMEDPATGIAHVHTVLQGLGLSPQASDAELVQRVCMAVCTRAAQLCASALAAVLSRLQHSREQQTLH
  VAVATGGRVFEWHPRFLCILKETVMLLAPECDVSFIPSVDGGGRGVAMVTAVAARLATHRRILEETLAPF
  QLSLEQLTAVQAQMREAMIRGLQGESSSLRMLPTYVRATPDGSERGDFLALDLGGTNFRVLLVRVAEGSV
  QITNQVYSIPEYVAQGSGQKLFDHIVDCIVDFQKRQGLSGQSLPLGFTFSFPCKQLGLDQGILLNWTKGF
  NASGCEGQDVVYLLREAIRRRQAVELNVVAIVNDTVGTMMSCGYDDPCCEMGLIVGTGTNACYMEELRNV
  ASVPGDSGHMCINMEWGAFGDDGSLSMLGTCFDASVDQASINPGKQRFEKMISGMYLGEIVRHILLHLTS
  LGVLFRGQKTQCLQTRDIFKTKFLSEIESDSLALRQVRAILEDLGLTLTSDDALMVLEVCQAVSRRAAQL
  CGAGVAAVVEKIRENRGLQELTVSVGVDGTLYKLHPHFSRLVSVTVRKLAPQCTVTFLQSEDGSGKGAAL
  VTRVACRLTQMACV
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022179
• ORF Size: 2772 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_022179.1, NM_022179.2, NP_071515.1
• RefSeq Size: 3027
• RefSeq ORF: 2775
• Locus ID: 25060
• MW: 100.3 kDa
• Gene Summary: ATP:D-hexose 6-phosphotransferase type III isozyme; member of a family of hexokinase isozymes [RGD, Feb 2006]