Fgd1 (NM_001037546) Rat Tagged ORF Clone

CAT#: RR207492

Fgd1 (Myc-DDK-tagged ORF) - Rat FYVE, RhoGEF and PH domain containing 1 (Fgd1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fgd1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207492 representing NM_001037546
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCCGGGTGCTTCCTCCTCCTGAGCCCATTCCTCCGCCACCATCACGCCCTCTGCCTGCAGACCCTA
  GAGTGGCCAAGGGTCTTGCCCCCAGAGCTGAGGCCAGCACTAGTTCTGCAGCAGTATCATCATTGATCGA
  GAAGTTTGAAAGAGAGCCTGTGATTGTCGCTTCGGATAGGCCAGCCCCTGGCCCCTGCCCAGTTCCCCCA
  GAGCCAGCCATGTTGCCACAGCCACCCCCGCAGCCAACAGGGTCCCAGCTCCCTGAGGGTGAGGCTTCTC
  GCTGCCTGTTCCTGCTGGCTCCTGGGCCCCGGGATGGTGAGAAGGTGCCTAACCGGGACAGCGGCATTGA
  CAGCATCAGCTCGCCATCCAACAGTGAAGAGACCTGCTTCGTCAGTGATGACGGGCCCCCCAGCCACAGC
  CTCTGTCCTGGGCCCCCTGCCCTGGCTAGTATGCCAGTGGCTTTGGCCGACCCCCACCGGCCTGGCTCCC
  AGGAGGTTGACAGTGACCTGGAAGAGGAAGAGGAGGAGGAGGAAGAAGAGGAGGAAAAGGAAAGAGAAAT
  TCCAGTGCCCCCGATGGAGAGACAGGAGTCTGTGGAGCTGACCGTGCAGCAGAAGGTATTCCATATTGCC
  AATGAACTCCTGCAAACTGAGAAGGCCTATGTTTCCAGGCTTCATCTCCTGGATCAGGTATTCTGTGCCC
  GGCTGCTGGAAGAAGCTCGGAACCGCAGTTCCTTCCCTGCAGATGTTGTCCATGGCATCTTCTCTAACAT
  CTGCTCCATCTACTGCTTCCATCAGCAGTTCCTGCTGCCTGAGCTGGAGAAGCGCATGGAGGAATGGGAC
  CGCTACCCACGCATTGGTGACATCCTTCAGAAGTTGGCACCCTTCCTCAAGATGTATGGCGAGTATGTGA
  AAAACTTTGACCGGGCTGTAGAGCTGGTCAACACATGGACAGAGCGTTCTACCCAGTTTAAAGTCATCAT
  CCATGAAGTACAGAAGGAGGAAGCCTGTGGCAACCTGACCCTGCAGCACCACATGCTGGAGCCCGTGCAG
  CGTATCCCCCGCTATGAGCTTCTTCTTAAGGACTATCTGTTAAAGCTGCCTCATGGCTCCCCGGACAGCA
  AGGATGCCCAGAAGTCTTTGGAGCTGATAGCCACAGCAGCAGAACACTCTAATGCTGCCATCCGTAAAAT
  GGAACGAATGCACAAGCTGCTGAAGGTCTATGAGCTGCTAGGGGGTGAGGAGGACATTGTCAGTCCCACC
  AAAGAGCTCATAAAAGAAGGTCATATCCTTAAGCTGTCAGCAAAGAATGGGACCACTCAAGATCGATACC
  TCATATTATTCAACGACCGCCTTCTTTACTGCGTGCCCAGGCTGCGGCTCCTTGGCCAGAAGTTTAGCGT
  GCGGGCACGCATTGATGTAGATGGCATGGAGCTAAAGGAAAGCTCCAACCTCAACATGCCTCGGACCTTC
  TTGGTGTCAGGAAAACAGCGCTCTCTTGAACTCCAGGCCAGGACTGAGGAGGAGAAGAAAGACTGGGTCC
  AGGCCATCAATTCTACCCTCCTGAAGCATGAACAGACCCTGGAGACTTTCAAACTGTTGAACTCAACAAA
  CAGGGATGATGAAGATACTCCCCCTAACTCTCCAAACGTGGATCTTGGGAAGAGGGCACCTACGCCTATC
  CGGGAAAAGGAAGTCACCATGTGCATGCGCTGCCAGGAGCCCTTCAATTCCATCACCAAACGCAGGCACC
  ACTGCAAGGCCTGTGGGCATGTGGTTTGTGGGAAATGCTCTGAGTTCCGAGCACGACTCATCTATGACAA
  CAATCGTTCCAACCGTGTGTGCACTGATTGCTATGTGGCCTTGCATGGAGCACCCGGGAGCAGTCCAGCC
  TGTAGCCAACATACACCCCAGCGCAGGAGGTCCATCCTAGAGAAACAGGCCTCAGTGGCTGCTGAGAACA
  GCGTCATCTGCAGCTTCCTGCACTACATGGAGAAGGGTGGGAAAGGATGGCACAAGGCATGGTTTGTGGT
  TCCCGAGAATGAACCCTTGGTGCTGTACATCTATGGAGCCCCTCAGGATGTGAAAGCCCAACGCAGCCTG
  CCCCTCATTGGCTTTGAGGTGGGGCCTCCTGAGGCAGGGGAGCGGCCAGACAGGAGGCATGTCTTCAAGA
  TCACCCAGAGCCACCTAAGCTGGTACTTCAGCCCAGAGACAGAAGAACTCCAGCGGCGCTGGATGGCTGT
  GCTTGGCAGGGCGGGCCGTGGGGACACATTCTGCCCAGGGCCCACACTGTCTGAGGACAAGGAGATGGAG
  GAGACAACAGTGGCAGCTTCAGGAGCCACTGCTGAACCTCCTGAAGCCTCCCAGACCCGAGACAAGACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207492 representing NM_001037546
  Red=Cloning site Green=Tags(s)
  
  MPRVLPPPEPIPPPPSRPLPADPRVAKGLAPRAEASTSSAAVSSLIEKFEREPVIVASDRPAPGPCPVPP
  EPAMLPQPPPQPTGSQLPEGEASRCLFLLAPGPRDGEKVPNRDSGIDSISSPSNSEETCFVSDDGPPSHS
  LCPGPPALASMPVALADPHRPGSQEVDSDLEEEEEEEEEEEEKEREIPVPPMERQESVELTVQQKVFHIA
  NELLQTEKAYVSRLHLLDQVFCARLLEEARNRSSFPADVVHGIFSNICSIYCFHQQFLLPELEKRMEEWD
  RYPRIGDILQKLAPFLKMYGEYVKNFDRAVELVNTWTERSTQFKVIIHEVQKEEACGNLTLQHHMLEPVQ
  RIPRYELLLKDYLLKLPHGSPDSKDAQKSLELIATAAEHSNAAIRKMERMHKLLKVYELLGGEEDIVSPT
  KELIKEGHILKLSAKNGTTQDRYLILFNDRLLYCVPRLRLLGQKFSVRARIDVDGMELKESSNLNMPRTF
  LVSGKQRSLELQARTEEEKKDWVQAINSTLLKHEQTLETFKLLNSTNRDDEDTPPNSPNVDLGKRAPTPI
  REKEVTMCMRCQEPFNSITKRRHHCKACGHVVCGKCSEFRARLIYDNNRSNRVCTDCYVALHGAPGSSPA
  CSQHTPQRRRSILEKQASVAAENSVICSFLHYMEKGGKGWHKAWFVVPENEPLVLYIYGAPQDVKAQRSL
  PLIGFEVGPPEAGERPDRRHVFKITQSHLSWYFSPETEELQRRWMAVLGRAGRGDTFCPGPTLSEDKEME
  ETTVAASGATAEPPEASQTRDKT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001037546
• ORF Size: 2379 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001037546.1, NP_001032635.1
• RefSeq Size: 3289 bp
• RefSeq ORF: 2382 bp
• Locus ID: 363460
• Cytogenetics: Xq12
• MW: 89.2 kDa