Ihh (NM_053384) Rat Tagged ORF Clone

CAT#: RR207510

Ihh (Myc-DDK-tagged ORF) - Rat Indian hedgehog (Ihh), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ihh
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207510 representing NM_053384
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCCCGCCTGGCTCCGGCCCCGACTGCGCTTCTGTCTGCTCCTGCTGCTGCTGCTTCTGGTGCCGG
  CGGCGCGGGGCTGCGGGCCGGGCCGGGTGGTGGGCAGCCGCCGGAGGCCGCCTCGTAAACTCGTGCCTCT
  TGCCTACAAGCAGTTCAGCCCCAACGTGCCGGAGAAGACCCTGGGCGCCAGCGGGCGCTACGAAGGCAAG
  ATCGCGCGCAGCTCTGAGCGCTTCAAGGAGCTCACCCCCAACTACAATCCCGACATCATCTTCAAAGACG
  AGGAGAACACCGGTGCCGACCGCCTCATGACCCAGCGCTGCAAGGACCGTCTGAACTCACTGGCCATCTC
  TGTCATGAACCAGTGGCCGGGTGTGAAGCTGCGGGTGACGGAAGGCTGGGATGAAGACGGCCATCACTCA
  GAGGAATCTTTACACTATGAGGGCCGCGCGGTGGATATCACCACCTCAGACCGCGACCGAAATAAGTACG
  GACTACTGGCGCGCTTAGCAGTGGAGGCCGGCTTCGACTGGGTGTATTACGAGTCCAAGGCCCACGTTCA
  TTGCTCTGTCAAGTCTGAGCACTCGGCTGCTGCCAAGACAGGTGGCTGCTTTCCTGCCGGAGCCCAGGTC
  CACCTAGAAACTGGGGAGCGTGTGGCCCTGTCAGCTGTGAAGCCAGGAGACCGGGTCCTGGCCATGGGGG
  AAGATGGCAACCCCACCTTCAGCGATGTGCTCATTTTCCTGGACCGTGAGCCAAACAGGCTGAGAGCTTT
  CCAGGTCATCGAGACTCAGGATCCTCCACGTCGGCTGGCACTCACGCCTGCCCACCTGCTCTTCATTGCG
  GACAATCATACAGAACCAGCAGCCCGCTTCCGGGCCACGTTTGCCAGCCACGTGCAGCCAGGCCAATATG
  TGCTGGTAGCAGGGGTCCCAGGCCTCCAGCCTGCTCGGGTGGCAGCCATCTCCACCCACGTGGCCCTTGG
  GTCCTATGCTCCTCTCACGAGGCACGGGACACTCGTGGTGGAGGATGTGGTAGCCTCCTGCTTCGCAGCT
  GTGTCTGACCACCATCTGGCTCAGTTGGCCTTCTGGCCCCTGCGACTGTTTCCCAGCTTGCCATGGGGCA
  GCTGGACCCCAAGTGAAGGTGTTCACTGGTACCCTCAGCTGCTCTACCGCCTGGGGCGTCTCTTGCTAGA
  AGAGAGCACCTTCCATCCACTGGGCATGTCTGGGGCAGGAAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207510 representing NM_053384
  Red=Cloning site Green=Tags(s)
  
  MSPAWLRPRLRFCLLLLLLLLVPAARGCGPGRVVGSRRRPPRKLVPLAYKQFSPNVPEKTLGASGRYEGK
  IARSSERFKELTPNYNPDIIFKDEENTGADRLMTQRCKDRLNSLAISVMNQWPGVKLRVTEGWDEDGHHS
  EESLHYEGRAVDITTSDRDRNKYGLLARLAVEAGFDWVYYESKAHVHCSVKSEHSAAAKTGGCFPAGAQV
  HLETGERVALSAVKPGDRVLAMGEDGNPTFSDVLIFLDREPNRLRAFQVIETQDPPRRLALTPAHLLFIA
  DNHTEPAARFRATFASHVQPGQYVLVAGVPGLQPARVAAISTHVALGSYAPLTRHGTLVVEDVVASCFAA
  VSDHHLAQLAFWPLRLFPSLPWGSWTPSEGVHWYPQLLYRLGRLLLEESTFHPLGMSGAGS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053384
• ORF Size: 1233 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_053384.1, NP_445836.1
• RefSeq Size: 2272
• RefSeq ORF: 1236
• Locus ID: 84399
• MW: 45.5 kDa
• Gene Summary: member of the hedgehog family of secreted morphogens; regulates chondrocyte (cartilage cell) differentiation [RGD, Feb 2006]