Apaf1 (NM_023979) Rat Tagged ORF Clone

CAT#: RR207514

Apaf1 (Myc-DDK-tagged ORF) - Rat apoptotic peptidase activating factor 1 (Apaf1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Apaf1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207514 representing NM_023979
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGCAAAGGCCCGAAATTGTTTGCTTCAACATAAAGAAGCTCTGGAAAAGGACATCAAAACATCCT
  ACATCATGGATCACATGATCAGTAATGGCGTCTTAACAGTAGTAGAGGAGGAAAAGGTCAAAAGTCAGGC
  CACTCAATATCAACGAGCAGCTGCTTTAATTAAAATGATACTTAATAAAGACAATTATGCCTACATTTCA
  TTCTACAACGCTCTACTACACGAGGGCTATAAAGACCTTGCTGGACTTCTGCATAGCGGCCTTCCTCTTG
  TGTCTTCTTCCAGCGGCAAGGACACAGACGGTGGAAACACTTCATTTGTAAGGACAGTGCTGTGTGAAGG
  TGGAGTGCCACAGAGGCCAGTCATCTTTGTCACTAGGAAGAAGCTGGTCAGTGCGATTCAGCAGAAGCTC
  TGGAAACTGAATGGGGAGCCAGGGTGGGTCACCATCTATGGAATGGCAGGCTGTGGCAAGTCTGTATTAG
  CTGCGGAAGCTGTTCGCGACCATGCCCTCCTAGAAGGTTGTTTTTCAGGGGGCGTACACTGGGTTTCCAT
  CGGAAAACAAGACAAGTCCGGGCTTCTAATGAAGCTGCAGAATCTTTGCACGCGGTTGGGTCAAGAAGAG
  AGTTTCTCTCAGAGGCTCCCGCTTAACATTGAGGAGGCCAAAGACCGTCTCCGTGTTCTGATGCTGCGAA
  AACACCCAAGGTCTCTGTTGATCTTGGATGACGTTTGGGATCCTTGGGTGTTAAAGGCTTTTGACAACCA
  GTGTCAGATTCTTCTTACCACCAGAGATAAGAGTGTTACCGATTCAGTAATGGGTCCTAAGTATGTTATC
  CCTGTGGAGAGTGGTCTAGGGAAAGAGAAGGGACTTGAAATCTTGTCACTTTTTGTTAATATGAAGAAAG
  AAGATCTGCCAGTGGAGGCTCACAGTATTATAAAGGAGTGCAAAGGCTCTCCTCTCGTCGTGTCTTTAGT
  TGGTGCGCTTTTACGGGATTTTCCCAATCGCTGGGCATACTACCTCAGGCAGCTTCAGAATAAGCAGTTT
  AAGAGAATAAGGAAATCTTCATCTTATGACTACGAGGCTCTGGATGAAGCCATGTCCATAAGTGTGGAGA
  TGCTCAGAGAAGACATCAAGGACTACTACACAGACCTCTCCATCCTTCAGAAGGATGTCAAGGTGCCTAC
  GAAGGTGCTGTGTGTTCTCTGGGACTTGGAAACGGAAGAAGTTGAAGACATCTTGCAGGAGTTCGTTAAT
  AAGTCTCTCTTATTCTGTAATCGGAATGGGAAGTCATTTTGTTATTATTTACACGATCTTCAAGTAGATT
  TCCTTACAGAGAAGAATCGAAGTCAGCTCCAGGATCTGCACAGGAAGATGGTCACTCAATTTCAGAGGTA
  TCACCAGCCGCATACGCTGTCTCCAGGTCAGGAGGACTGCATGTACTGGTACAACTTCCTGGCCTATCAT
  ATGGCCAGTGCCGGTATGCACAAAGAACTTTGTGCTTTAATGTTTTCCCTGGATTGGATTAAAGCAAAAA
  CAGAACTTGTCGGCCCTGCGCATCTGATTCATGAGTTCGTGGAATATAGGCATATACTGGATGAAAAGGA
  TTGTGCAGTCTGTGAGAATTTTCAGGAGTTTTTATCTTTAAACGGACACCTTCTTGGACGACAGCCATTT
  CCTAACATTGTGCAACTGGGCCTCTGTGAACCAGAGACTTCCGAAGTTTATCAACAAGCAAAGCTGCAGG
  CCAAGCAGGAGGTGGATACCGGGCGGCTTTACCTGGAATGGATCAACAAAAAAACCATCAAGAATCTGTC
  CCGCTTAGTCGTTCGCCCCCATACAGACGCCGTGTACCATGCGTGTTTTTCTCAGGATGGTCAGAGAATA
  GCTTCTTGTGGAGCTGATAAAACCTTGCAGGTGTTTAAAGCCGAGACAGGAGAGAAACTTCTTGACATTA
  AAGCTCATGAAGATGAGGTGCTTTGCTGTGCGTTCTCCTCAGATGACAGTTACATAGCAACCTGCTCAGT
  GGATAAGAAAGTTAAGATTTGGGATTCTGGGACTGGGAAGCTAGTGCACACCTACGAAGAACACTCAGAG
  CAGGTCAATTGCTGCCATTTCACCAATAAGAGCAACCACCTTCTTTTGGCCACCGGGTCAAATGACTCCT
  TCCTTAAGCTCTGGGATTTGAATCAAAAAGAATGCCGAAATACCATGTTCGGTCACACGAACTCAGTCAC
  CCACTGCAGGTTCTCACCAGACGATGAGCTCTTGGCGAGTTGCTCAGCCGACGGGACTTTAAAGCTTTGG
  GATGTGAGATCAGCAAACGAGAAGAAAAGCATTAATGTGAAGCGCTTCTTCCTGAGTTCAGAAGACCCAC
  CAGAGGACGTGGAGGTGATCGTGAAATGTTGCTCCTGGTCTGCAGATGGCGACAGAATAATAGTGGCAGC
  AAAAAACAAAGTCCTCCTTCTTGATATTCATACTAGTGGCCTGCTGACTGAGATCCACACAGGTCATCAC
  AGCACCATCCAGTACTGTGACTTCTCCCCCTACGACCATTTGGCAGTGATTGCCCTGTCTCAGTACTGTG
  TGGAGTTGTGGAACATAGACTCCCGGGTAAAGGTGGCTGACTGCAGGGGACATTTAAGTTGGGTCCACGG
  TGTGATGTTTTCTCCTGATGGCTCCTCGTTCTTGACAGCTTCTGATGACCAAACAATAAGGGTCTGGGAG
  ACAAGAAAGGTGTGCAAGAACTCTGCCATTGTGTTAAAGCAGGAAATAGATGTCGTGTTTCAAGAAAACG
  AAATGATGGTCCTTGCAGTTGACAACATAAGAGGCCTACAACTCATTGCTGGAAAAACAGGCCAGATTGA
  TTACCTGCCTGAAGCCCAAGTGAGTTGCTGCTGCCTAAGTCCACATCTTGAGTACGTTGCATTTGGAGAT
  GAAGAGGGAGCCATTAAGATTATAGAACTTCCAAACAACAGAGTTTTCAGCTCTGGGATTGGGCACAAGA
  AAGCTGTACGGCACATCCAGTTCACAGCTGATGGGAAGACACTGATTTCAAGTTCTGAAGACTCTGTGAT
  TCAGGTGTGGAATTGGCAGACAGAGGAGTATGTATTTCTGCAAGCCCATCAGGAAACAGTAAAGGACTTC
  AGACTCCTGAGAGATTCACGATTGCTTTCCTGGTCATTCGATGGAACGGTGAAGGTGTGGAATGTCATTA
  CCGGAAGAATTGAAAGAGACTTTACTTGTCATCAGGGCACAGTGCTTTCCTGTGCTATCTCTTCTGATGC
  CACCAAGTTTTCATCTACCTCAGCTGATAAGACCGCCAAGATCTGGAGTTTTGAACTCCCTTCCCCTCTT
  CATGAGCTGAAGGGCCATAACAGCTGTGTCCGCTGCTCTGCCTTCTCGCTGGATGGCATCCTGTTAGCAA
  CTGGAGATGACAATGGAGAAATCAGGATATGGAATGTCTCAGATGGCCAGCTTCTTCATTTGTGTGCTCC
  GATTTCCATAGAAGAAGGAACGGCTACCCACGGAGGCTGGGTGACTGATGTTTGCTTCTCTCCTGACAGA
  AAAATGCTTGTCTCTGCCGGAGGATACCTTAAGTGGTGGAATGTTGTTACTGGGGAGTCCTCACAGACCT
  TCTACACCAATGGAACAAACCTCAAGAAAATACATGTGTCCCCTGACTTCAGAACATATGTGACCGTTGA
  TAATCTTGGTATTTTATATATTTTACAGGTTTTAGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207514 representing NM_023979
  Red=Cloning site Green=Tags(s)
  
  MDAKARNCLLQHKEALEKDIKTSYIMDHMISNGVLTVVEEEKVKSQATQYQRAAALIKMILNKDNYAYIS
  FYNALLHEGYKDLAGLLHSGLPLVSSSSGKDTDGGNTSFVRTVLCEGGVPQRPVIFVTRKKLVSAIQQKL
  WKLNGEPGWVTIYGMAGCGKSVLAAEAVRDHALLEGCFSGGVHWVSIGKQDKSGLLMKLQNLCTRLGQEE
  SFSQRLPLNIEEAKDRLRVLMLRKHPRSLLILDDVWDPWVLKAFDNQCQILLTTRDKSVTDSVMGPKYVI
  PVESGLGKEKGLEILSLFVNMKKEDLPVEAHSIIKECKGSPLVVSLVGALLRDFPNRWAYYLRQLQNKQF
  KRIRKSSSYDYEALDEAMSISVEMLREDIKDYYTDLSILQKDVKVPTKVLCVLWDLETEEVEDILQEFVN
  KSLLFCNRNGKSFCYYLHDLQVDFLTEKNRSQLQDLHRKMVTQFQRYHQPHTLSPGQEDCMYWYNFLAYH
  MASAGMHKELCALMFSLDWIKAKTELVGPAHLIHEFVEYRHILDEKDCAVCENFQEFLSLNGHLLGRQPF
  PNIVQLGLCEPETSEVYQQAKLQAKQEVDTGRLYLEWINKKTIKNLSRLVVRPHTDAVYHACFSQDGQRI
  ASCGADKTLQVFKAETGEKLLDIKAHEDEVLCCAFSSDDSYIATCSVDKKVKIWDSGTGKLVHTYEEHSE
  QVNCCHFTNKSNHLLLATGSNDSFLKLWDLNQKECRNTMFGHTNSVTHCRFSPDDELLASCSADGTLKLW
  DVRSANEKKSINVKRFFLSSEDPPEDVEVIVKCCSWSADGDRIIVAAKNKVLLLDIHTSGLLTEIHTGHH
  STIQYCDFSPYDHLAVIALSQYCVELWNIDSRVKVADCRGHLSWVHGVMFSPDGSSFLTASDDQTIRVWE
  TRKVCKNSAIVLKQEIDVVFQENEMMVLAVDNIRGLQLIAGKTGQIDYLPEAQVSCCCLSPHLEYVAFGD
  EEGAIKIIELPNNRVFSSGIGHKKAVRHIQFTADGKTLISSSEDSVIQVWNWQTEEYVFLQAHQETVKDF
  RLLRDSRLLSWSFDGTVKVWNVITGRIERDFTCHQGTVLSCAISSDATKFSSTSADKTAKIWSFELPSPL
  HELKGHNSCVRCSAFSLDGILLATGDDNGEIRIWNVSDGQLLHLCAPISIEEGTATHGGWVTDVCFSPDR
  KMLVSAGGYLKWWNVVTGESSQTFYTNGTNLKKIHVSPDFRTYVTVDNLGILYILQVLE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_023979
• ORF Size: 3747 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_023979.1, NP_076469.1
• RefSeq Size: 6111
• RefSeq ORF: 3750
• Locus ID: 78963
• MW: 141.2 kDa
• Gene Summary: protein involved in the initiation of apoptosis [RGD, Feb 2006]