Atg7 (NM_001012097) Rat Tagged ORF Clone

CAT#: RR207526

Atg7 (Myc-DDK-tagged ORF) - Rat ATG7 autophagy related 7 homolog (S. cerevisiae) (Atg7), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Atg7
• Synonyms: Apg7l
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207526 representing NM_001012097
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGACCCTGGACTGTCCAAGCTGCAGTTTGCCCCCTTTAATAGTGCCCTGGACGTTGGCTTCTGGC
  ACGAACTGACCCAGAAGAAGTTGAACGAGTATCGCCTGGACGAGGCACCCAAAGACATCAAGGGCTATTA
  CTACAATGGTGACTCTGCCGGCCTCCCCACCCGCTTGACATTGGAGTTCAGTGCTTTTGACATGAGCGCC
  CCCACGCCTGCCCGCTGCTGCCCGGCCATGGGAACCCTGCACAACACCAACACACTTGAGGCTTTTAAGA
  CTGCAGACAAGAAGCTCCTTCTGGAGCAGTCAGCCAATGAGATCTGGGAAGCCATAAAGTCAGGTGCTGC
  TCTCGAAAACCCCATGCTCCTCAACAAGTTTCTGCTCTTGACCTTCGCGGACCTAAAGAAGTACCACTTC
  TACTACTGGTTTTGCTGCCCTGCCCTCTGTCTTCCTGAGAGCATCCCTCTAATCCGGGGACCTGTGGGCT
  TGGATCAAAGGCTTTCACCAAAACAGATCCAGGCCCTGGAGCATGCCTACGATGACCTGTGTCGAACTGA
  AGGCGTCACAGCCCTGCCATACTTCTTATTCAAGTACGATGACGACACTGTGCTGGTCTCCTTGCTCAAA
  CACTACAGTGATTTCTTCCAAGGTCAAAGGACAAAGTTAACAGTCGGTGTGTACGATCCCTGTAACCTAA
  CCCAGCACCCTGGATGGCCTTTGAGGAATTTTTTGGTCCTGGCAGCCCACAGATGGAGCGGCAGTTTCCA
  GTCTGTTGAAGTTCTCTGCTTTCGGGACCGCACCATGCAGGGAGCAAGAGATGTGACACACAGCATCATC
  TTTGAAGTGAAACTTCCAGAAATGGCGTTTAGCCCAGATTGTCCTAAGGCAGTTGGCTGGGAGAAGAACC
  AGAAAGGAGGCATGGGTCCGAGGATGGTGAACCTCAGCGGATGTATGGACCCCAAAAGGCTGGCTGAGTC
  ATCCGTGGATCTGAATCTCAAGCTGATGTGCTGGCGGTTGGTCCCCACCTTGGACTTGGACAAGGTCGTG
  TCTGTCAAGTGCCTGCTGCTGGGAGCTGGTACCTTGGGGTGTAATGTGGCTAGAACACTGATGGGCTGGG
  GCGTCAGACACGTCACGTTTGTGGACAACGCCAAGATCTCCTACTCCAATCCCGTGAGGCAGCCTCTGTA
  TGAATTTGAAGATTGTCTAGGGGGTGGCAAGCCCAAGGCCCTGGCTGCAGCAGAGCGGCTCCAGAAAATA
  TTTCCCGGAGTGAATGCCAGCGGGTTCAACATGAGCATCCCCATGCCCGGACACCCTGTGAACTTCTCTG
  ACGTCACGATGGAGCAGGCCCGCAGAGATGTGGAGCAGCTGGAGGAACTCATCGATAGCCACGATGTCAT
  CTTCCTGCTAATGGACACCAGGGAGAGCCGATGGCTTCCTACTGTTATTGCAGCCAGCAAGCGAAAGCTG
  GTCATCAATGCTGCCTTGGGGTTTGACACCTTTGTTGTCATGAGACATGGCCTGAAGAAACCCAAGCAGC
  AGGGAGCCGGGGACCTGTGCCCAAGCCATCTTGTAGCACCTGCTGACCTGGGCTCCTCACTTTTTGCCAA
  CATCCCTGGATACAAGCTTGGCTGCTACTTCTGCAATGATGTGGTGGCTCCAGGAGATTCCACCAGAGAC
  CGGACTCTGGACCAGCAGTGCACAGTGAGCCGCCCAGGGCTGGCCGTGATTGCAGGAGCCCTGGCTGTGG
  AGCTGATGGTTTCTGTCTTGCAGCATCCTGAGGGGGGCTACGCCATCGCCAGCAGCAGTGATGACCGCAT
  GAATGAGCCTCCCACCTCGCTGGGGCTTGTACCTCACCAGATCCGGGGCTTTCTGTCACGGTTCGATAAT
  GTCCTTCCTGTCAGCCTGGCATTTGATAAATGTACAGCCTGTTCATCCAAAGTTCTTGATCAATATGAGC
  AAGAAGGATTCACCTTCCTGGCCAAGGTGTTTAACTCTTCACATTCCTTCTTAGAAGATTTGACTGGTCT
  TACACTGCTCCACCAAGAGACCCAAGCTGCTGAGATCTGGGACATGAGTGACGAGGAGACCGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207526 representing NM_001012097
  Red=Cloning site Green=Tags(s)
  
  MGDPGLSKLQFAPFNSALDVGFWHELTQKKLNEYRLDEAPKDIKGYYYNGDSAGLPTRLTLEFSAFDMSA
  PTPARCCPAMGTLHNTNTLEAFKTADKKLLLEQSANEIWEAIKSGAALENPMLLNKFLLLTFADLKKYHF
  YYWFCCPALCLPESIPLIRGPVGLDQRLSPKQIQALEHAYDDLCRTEGVTALPYFLFKYDDDTVLVSLLK
  HYSDFFQGQRTKLTVGVYDPCNLTQHPGWPLRNFLVLAAHRWSGSFQSVEVLCFRDRTMQGARDVTHSII
  FEVKLPEMAFSPDCPKAVGWEKNQKGGMGPRMVNLSGCMDPKRLAESSVDLNLKLMCWRLVPTLDLDKVV
  SVKCLLLGAGTLGCNVARTLMGWGVRHVTFVDNAKISYSNPVRQPLYEFEDCLGGGKPKALAAAERLQKI
  FPGVNASGFNMSIPMPGHPVNFSDVTMEQARRDVEQLEELIDSHDVIFLLMDTRESRWLPTVIAASKRKL
  VINAALGFDTFVVMRHGLKKPKQQGAGDLCPSHLVAPADLGSSLFANIPGYKLGCYFCNDVVAPGDSTRD
  RTLDQQCTVSRPGLAVIAGALAVELMVSVLQHPEGGYAIASSSDDRMNEPPTSLGLVPHQIRGFLSRFDN
  VLPVSLAFDKCTACSSKVLDQYEQEGFTFLAKVFNSSHSFLEDLTGLTLLHQETQAAEIWDMSDEETV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012097
• ORF Size: 2094 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012097.1, NP_001012097.1
• RefSeq Size: 2386 bp
• RefSeq ORF: 2097 bp
• Locus ID: 312647
• Cytogenetics: 4q42
• MW: 77.4 kDa