Limk2 (NM_024135) Rat Tagged ORF Clone

CAT#: RR207531

Limk2 (Myc-DDK-tagged ORF) - Rat LIM domain kinase 2 (Limk2), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Limk2
• Synonyms: Limk2b; Link2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207531 representing NM_024135
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGCTGGCGGGTGAAGAGGCCTGGAGGTGTCGGGGCTGTGGCAACTATGTTCCCCTGAGCCAGA
  GGCTGTACAGGACAGCCAACGAGGCCTGGCATAGCTCCTGCTTCCGGTGTTCCGAGTGCCAGGAGTCCCT
  CACCAACTGGTACTATGAGAAGGACGGGAAGCTCTACTGCCACAAGGACTACTGGGCCAAGTTTGGAGAA
  TTCTGCCACGGGTGCTCCCTGCTGATGACAGGGCCAGCCATGGTGGCTGGGGAGTTTAAGTACCACCCAG
  AGTGCTTTGCCTGCATGAGTTGCAAGGTGATCATCGAGGATGGGGATGCGTACGCCCTGGTGCAGCATGC
  TACACTCTACTGCGGGAAGTGCCACAATGAGGTGGTGCTGGCACCCATGTTTGAAAGACTGTCTACGGAG
  TCTGTCCAGGACCAGCTGCCCTACTCTGTCACGCTCATCTCCATGCCTGCCACCACCGAGTGCAGACGTG
  GCTTCTCTGTGTCTGTGGAAAGCGCCTCCTCTAACTATGCCACCACCGTGCAAGTGAAAGAGGTCAACCG
  GATGCACATCAGCCCCAACAACCGAAATGCCATCCACCCCGGGGACCGCATCTTGGAGATCAATGGGACC
  CCTGTCCGCACTCTCCGAGTAGAAGAGGTGGAGGATGCGATTAACCAGACGAGCCAGACACTTCAACTGC
  TGATTGAACATGACCCTGTCCCCCAGCGCCTGGACCAGTTACGGCTAGACACCCGGCTTTCTCCCCACAT
  GCAGAGTAGTGGACATACTCTCATGCTCAGCACCTTGGACGCCAAGGAAAATCAGGAGGGAACACTGAGG
  AGACGTTCTCTGAGGCGCAGTAACAGCATCTCCAAGTCTCCTGGCCCCAGCTCCCCCAAGGAGCCGCTGC
  TCCTGAGCCGAGACATCAGTCGCTCAGAGTCCCTTCGCTGCTCCAGCAGCTACTCACAGCAGATCTTCCG
  GCCCTGTGACCTGATCCACGGGGAGGTCCTGGGGAAGGGCTTCTTCGGACAGGCCATCAAGGTGACTCAC
  AAAGCCACAGGCAAAGTGATGGTCATGAAGGAGTTAATTCGCTGTGATGAGGAAACCCAGAAGACTTTCC
  TGACTGAGGTGAAAGTGATGCGAAGCCTGGACCACCCTAACGTGCTTAAGTTCATCGGGGTCCTGTACAA
  GGACAAGAAGCTGAATCTGCTGACAGAGTACATTGAGGGGGGCACCCTGAAGGACTTTCTGCGCAATGTG
  GACCCGTTTCCCTGGCAACAGAAGGTCAGGTTTGCCAAAGGCATCGCTTCCGGAATGGCTTATTTACACT
  CCATGTGCATCATCCACCGAGATCTGAACTCGCACAACTGTCTCATCAAATTGGACAAGACGGTGGTGGT
  AGCAGACTTCGGGCTGTCACGTCTCATAGTGGAAGAGAGGAAAAGGCCTCCAGTAGAGAAGGCCGCCACC
  AAGAAACGCACCTTACGCAAGAGTGATCGCAAGAAGCGCTACACTGTGGTGGGAAACCCCTACTGGATGG
  CCCCTGAGATGCTGAATGGCAAGAGCTACGATGAGACTGTGGATGTCTTCTCTTTTGGGATTGTTCTCTG
  TGAGATCATTGGGCAGGTATATGCTGATCCCGACTGCCTTCCCCGCACACTGGACTTCGGCCTCAACGTA
  AAGCTTTTCTGGGAGAAGTTTGTCCCAACAGACTGTCCCCCAGCCTTCTTCCCCCTGGCTGCTATCTGCT
  GCAAACTGGAGCCTGAGAGCAGACCGGCATTCTCAAAGCTGGAGGACTCATTCGAGGCGCTCTCCCTGTT
  CTTGGGAGAGTTGGCCATCCCACTGCCAGCAGAGCTGGAAGAGCTGGACCACACTGTGAGCATGGAGTAT
  GGCCTAACCCGGGACTCGCCACCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207531 representing NM_024135
  Red=Cloning site Green=Tags(s)
  
  MAALAGEEAWRCRGCGNYVPLSQRLYRTANEAWHSSCFRCSECQESLTNWYYEKDGKLYCHKDYWAKFGE
  FCHGCSLLMTGPAMVAGEFKYHPECFACMSCKVIIEDGDAYALVQHATLYCGKCHNEVVLAPMFERLSTE
  SVQDQLPYSVTLISMPATTECRRGFSVSVESASSNYATTVQVKEVNRMHISPNNRNAIHPGDRILEINGT
  PVRTLRVEEVEDAINQTSQTLQLLIEHDPVPQRLDQLRLDTRLSPHMQSSGHTLMLSTLDAKENQEGTLR
  RRSLRRSNSISKSPGPSSPKEPLLLSRDISRSESLRCSSSYSQQIFRPCDLIHGEVLGKGFFGQAIKVTH
  KATGKVMVMKELIRCDEETQKTFLTEVKVMRSLDHPNVLKFIGVLYKDKKLNLLTEYIEGGTLKDFLRNV
  DPFPWQQKVRFAKGIASGMAYLHSMCIIHRDLNSHNCLIKLDKTVVVADFGLSRLIVEERKRPPVEKAAT
  KKRTLRKSDRKKRYTVVGNPYWMAPEMLNGKSYDETVDVFSFGIVLCEIIGQVYADPDCLPRTLDFGLNV
  KLFWEKFVPTDCPPAFFPLAAICCKLEPESRPAFSKLEDSFEALSLFLGELAIPLPAELEELDHTVSMEY
  GLTRDSPP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024135
• ORF Size: 1914 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_024135.2, NP_077049.2
• RefSeq Size: 3455
• RefSeq ORF: 1917
• Locus ID: 29524
• MW: 72.2 kDa
• Gene Summary: kinase with a LIM-kinase (LIMK) domain; human homolog phosphorylates cofilin [RGD, Feb 2006]