Cwc27 (NM_001013199) Rat Tagged ORF Clone

CAT#: RR207602

Cwc27 (Myc-DDK-tagged ORF) - Rat serologically defined colon cancer antigen 10 (Sdccag10), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cwc27
• Synonyms: Sdccag10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207602 representing NM_001013199
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTAACATCTACATCCAGGAGCCGCCCACGAATGGGAAGGTTTTATTGAAAACTACGGCTGGAGATA
  TTGACATAGAGCTGTGGTCAAAAGAGGCCCCTAAAGCATGCAGAAATTTCATTCAGCTTTGTTTGGAGGC
  CTACTATGACAACACCATTTTTCATAGAGTCGTACCTGGCTTCATAGTCCAAGGTGGAGATCCCACAGGC
  ACCGGGACTGGTGGAGAATCTGTCTATGGAGCCCCATTCAAGGATGAGTTTCACTCACGGTTGCGTTTTA
  ATAGGAGAGGACTGGTTGCCATGGCAAATGCCGGTCCACATGATAATGGCAGCCAGTTTTTCTTTACCCT
  GGGTCGAGCAGATGAACTCAACAATAAGCACACCATCTTTGGAAAGGTTACAGGGGATACAGTGTACAAC
  ATGCTACGCCTGACAGAAGTAGACATCGATGATGAAGAAAGACCCCGCAATCCACATAGAATCAAGAGTT
  GTGAGGTTTTGTTTAACCCTTTTGATGACATCATTCCAAGGGAAATTAAAAAGCCCAAGAAAGAGAAGGC
  AGAGGAAGAAATCAAGAAATTGAAACCCAAAGGCACAAAAAATTTTAGTTTACTGTCTTTTGGAGAAGAA
  GCTGAGGAGGAGGAGGAGGAAGTGAACCGAGTCAGTCAGAGCATGAAGGGGAGAAGTAAAAGCAGCCATG
  ACCTGCTGAAGGACGATCCGCATCTCAGCTCTGTCCCGGCCGTCGAAAGTGAAAAAGATGATGCAACAGG
  AGATTTAGAAGATGATGCTGAAGATGACAGTGTAGAGCATGATGGCTCCATGGAGGAGGATGAGAAGAAC
  CTGATGAGAGAAAGAATCGCAAAACGGTTGAAGAAAGATGCAAGTGCCAATGTGAAGTCAGCCGGAGATG
  GGGAGAAGAAGCCCGCAAGCCGAAGTGAAGAGCTCAGAAAAGAAGCAAGGCAATTAAAGCGGGAACTCTT
  AGCAGCAAAACAGAAGAAAGAAAGTGCCACAAAAGCAGAGAAAGGAAGTGAAGAGGAAGAAGCTGTCCCA
  GATGGCCCTGTTGCAGAGTACAGGCGAGAAAAGCAGAAGTATGAAGCTTTGAGGAAGCAACAGCCCAAGA
  AGGGAACTTCCCGAGAGGATCAGACCCTGGCACTGCTGAGCCAGTTTAAATCTAAGCTCACTCAAGCCAT
  CACCGAAATGCCTGAAAACTGTGCTGAAGCAGAAGTTGAAGATGACGAAGGCTGGATGTCCCACGTGCTC
  CAGTTTGAAGACAAGACCCGGAAGGTGAAAGACGCAAGCATGCAGGACTCAGACACGTTTGAGATCTATG
  ATCCCCGGAATCCAGTGAACAAAAGAAGGAGGGAGGAAAGCAAAAAACTGCTGAGAGAGAAAAAGGAAAG
  GCGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207602 representing NM_001013199
  Red=Cloning site Green=Tags(s)
  
  MSNIYIQEPPTNGKVLLKTTAGDIDIELWSKEAPKACRNFIQLCLEAYYDNTIFHRVVPGFIVQGGDPTG
  TGTGGESVYGAPFKDEFHSRLRFNRRGLVAMANAGPHDNGSQFFFTLGRADELNNKHTIFGKVTGDTVYN
  MLRLTEVDIDDEERPRNPHRIKSCEVLFNPFDDIIPREIKKPKKEKAEEEIKKLKPKGTKNFSLLSFGEE
  AEEEEEEVNRVSQSMKGRSKSSHDLLKDDPHLSSVPAVESEKDDATGDLEDDAEDDSVEHDGSMEEDEKN
  LMRERIAKRLKKDASANVKSAGDGEKKPASRSEELRKEARQLKRELLAAKQKKESATKAEKGSEEEEAVP
  DGPVAEYRREKQKYEALRKQQPKKGTSREDQTLALLSQFKSKLTQAITEMPENCAEAEVEDDEGWMSHVL
  QFEDKTRKVKDASMQDSDTFEIYDPRNPVNKRRREESKKLLREKKERR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001013199
• ORF Size: 1404 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001013199.1, NP_001013217.1
• RefSeq Size: 1852 bp
• RefSeq ORF: 1407 bp
• Locus ID: 361887
• Cytogenetics: 2q12
• MW: 53.3 kDa