Grin2b (NM_012574) Rat Tagged ORF Clone

CAT#: RR207651

Grin2b (Myc-DDK-tagged ORF) - Rat glutamate receptor, ionotropic, N-methyl D-aspartate 2B (Grin2b), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Grin2b
• Synonyms: GluN2B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207651 representing NM_012574
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCCCAGCGCAGAGTGCTGTTCCCCCAAGTTCTGGTTGGTGTTGGCCGTCTTGGCCGTATCAGGCA
  GCAAAGCTCGTTCCCAAAAGAGCCCCCCCAGCATCGGCATCGCTGTCATCCTCGTGGGCACTTCAGACGA
  AGTGGCCATAAAAGACGCCCACGAGAAAGATGACTTCCATCATCTCTCAGTAGTTCCCCGGGTGGAGCTG
  GTAGCCATGAACGAAACTGACCCAAAGAGCATCATCACCCGTATCTGCGATCTTATGTCTGACCGGAAGA
  TCCAGGGGGTGGTGTTCGCGGATGACACCGACCAAGAAGCCATCGCTCAGATCCTCGACTTCATTTCTGC
  TCAGACTCTCACCCCCATCCTGGGCATCCATGGGGGCTCATCTATGATAATGGCGGATAAGGATGAGTCC
  TCCATGTTCTTCCAGTTTGGCCCGTCTATCGAACAGCAAGCTTCCGTCATGCTCAACATCATGGAAGAAT
  ATGACTGGTACATCTTTTCCATCGTCACCACCTACTTCCCTGGCTACCAGGACTTTGTGAACAAGATCCG
  CAGTACCATCGAGAACAGCTTCGTGGGCTGGGAGCTCGAGGAAGTCCTCCTGCTAGACATGTCTCTGGAC
  GATGGCGACTCTAAGATTCAGAATCAGCTGAAGAAGCTCCAAAGCCCCATCATTCTCCTTTATTGCACGA
  AGGAGGAAGCCACCTACATTTTTGAAGTAGCTAACTCAGTTGGGCTGACTGGCTACGGCTACACGTGGAT
  TGTGCCGAGTCTGGTGGCCGGGGATACGGACACGGTGCCTTCAGAGTTCCCCACGGGGCTTATCTCTGTG
  TCTTATGATGAATGGGACTATGGCCTTCCTGCCAGAGTGAGAGATGGAATTGCCATCATCACCACTGCTG
  CCTCGGACATGCTGTCCGAACACAGTTTCATCCCTGAGCCCAAGAGCAGTTGCTACAACACCCACGAGAA
  GAGGATCTACCAGTCTAACATGTTGAATAGGTATCTGATCAATGTCACTTTTGAAGGGAGAAACCTGTCC
  TTCAGCGAAGATGGCTACCAGATGCATCCGAAGCTGGTGATAATCCTTCTGAACAAGGAGAGGAAGTGGG
  AGAGGGTGGGGAAATGGAAGGACAAGTCCCTGCAGATGAAGTATTATGTGTGGCCTCGGATGTGTCCTGA
  GACTGAGGAGCAAGAGGATGACCATCTGAGCATTGTCACCTTGGAGGAGGCGCCATTTGTCATTGTGGAA
  AGCGTGGACCCTCTCAGTGGAACCTGCATGAGGAATACAGTCCCGTGCCAGAAGCGCATCATCTCTGAGA
  ATAAAACAGATGAGGAACCAGGCTACATCAAAAAATGCTGCAAGGGGTTCTGTATTGACATCCTTAAGAA
  AATTTCTAAGTCTGTGAAGTTCACCTATGACCTTTACCTGGTGACCAATGGCAAGCACGGGAAGAAGATT
  AATGGGACCTGGAATGGCATGATCGGTGAGGTGGTCATGAAGAGGGCCTACATGGCAGTGGGATCACTAA
  CTATCAATGAAGAACGGTCAGAGGTGGTTGACTTCTCTGTACCCTTCATAGAAACTGGCATCAGTGTCAT
  GGTATCTCGCAGCAATGGGACTGTGTCACCTTCTGCCTTCTTAGAGCCATTCAGCGCTGACGTGTGGGTG
  ATGATGTTTGTGATGCTGCTCATTGTTTCTGCGGTGGCTGTCTTTGTCTTTGAATACTTCAGCCCTGTGG
  GTTACAACAGGTGCCTAGCCGATGGCAGAGAGCCAGGAGGCCCATCTTTCACCATCGGCAAAGCAATTTG
  GTTACTCTGGGGTCTGGTGTTTAACAACTCCGTACCTGTGCAGAACCCAAAGGGGACCACCTCCAAGATC
  ATGGTGTCAGTGTGGGCCTTCTTTGCTGTCATTTTCCTGGCCAGCTACACTGCCAACTTAGCAGCCTTCA
  TGATCCAAGAGGAGTATGTGGACCAGGTTTCTGGCCTGAGTGACAAGAAGTTCCAGAGACCTAATGACTT
  CTCACCCCCTTTCCGCTTTGGGACTGTGCCCAATGGCAGCACAGAGAGGAATATCCGTAATAACTATGCA
  GAAATGCATGCCTACATGGGAAAGTTCAACCAAAGGGGTGTAGATGATGCATTGCTCTCCCTGAAAACAG
  GGAAGCTTGATGCATTCATCTATGATGCAGCTGTGCTCAACTACATGGCTGGAAGGGACGAAGGCTGCAA
  ACTGGTGACCATTGGCAGTGGCAAGGTCTTTGCTTCTACCGGCTATGGCATTGCTATCCAAAAGGACTCC
  GGGTGGAAGCGCCAGGTGGACCTGGCTATCCTGCAGCTGTTTGGAGATGGGGAGATGGAAGAACTGGAAG
  CTCTCTGGCTCACTGGCATTTGCCACAATGAGAAGAATGAGGTGATGAGCAGCCAGCTGGACATCGACAA
  TATGGCAGGTGTCTTCTATATGTTGGGGGCAGCCATGGCCCTCAGCCTCATCACCTTCATCTGTGAGCAT
  CTGTTCTATTGGCAGTTCCGGCATTGCTTCATGGGTGTCTGTTCTGGCAAGCCTGGCATGGTCTTCTCCA
  TCAGCAGAGGTATCTACAGCTGTATCCATGGGGTAGCCATAGAGGAGCGCCAATCCGTGATGAACTCCCC
  CACTGCCACCATGAACAACACCCACTCCAACATCCTACGCTTGCTCCGCACGGCCAAGAACATGGCCAAC
  CTGTCTGGAGTAAACGGCTCCCCTCAGAGTGCCCTGGACTTCATCCGCCGAGAGTCCTCCGTCTACGACA
  TCTCTGAGCATCGTCGCAGCTTCACGCATTCAGACTGCAAGTCTTACAATAACCCACCCTGTGAGGAAAA
  CCTGTTCAGTGACTACATTAGCGAGGTAGAGAGAACATTTGGTAACCTGCAGCTGAAGGACAGCAATGTG
  TACCAAGACCACTATCACCATCACCACCGGCCACACAGCATCGGCAGCACCAGCTCCATTGATGGGCTCT
  ATGACTGTGACAACCCACCCTTCACCACCCAGCCCAGGTCAATCAGCAAGAAACCCCTGGACATCGGCCT
  GCCCTCCTCCAAACATAGCCAGCTCAGCGACCTGTATGGCAAGTTCTCTTTCAAGAGTGACCGCTACAGT
  GGCCACGACGACTTGATTCGATCGGATGTCTCCGACATCTCCACGCACACTGTCACCTATGGGAACATCG
  AGGGCAACGCAGCCAAGAGGAGGAAACAGCAGTATAAGGACAGTCTAAAGAAGCGGCCAGCCTCGGCCAA
  ATCGAGGAGGGAGTTTGATGAAATCGAGCTGGCCTACCGTCGCCGACCACCCCGCTCCCCGGACCACAAG
  CGCTACTTCAGGGACAAAGAAGGGCTCCGAGACTTCTACCTGGACCAGTTCCGAACAAAGGAGAACTCGC
  CTCACTGGGAGCACGTGGACTTGACTGACATTTACAAAGAACGCAGTGACGACTTCAAGCGAGATTCGGT
  CAGTGGAGGTGGGCCCTGTACCAACAGGTCTCACCTCAAACACGGAACGGGCGAGAAGCACGGAGTGGTA
  GGCGGGGTGCCTGCTCCTTGGGAGAAGAACCTGACCAATGTGGATTGGGAGGACCGGTCTGGGGGCAACT
  TCTGCCGCAGCTGTCCTTCCAAGCTGCACAATTACTCCTCGACGGTGGCAGGGCAGAACTCGGGCCGGCA
  GGCCTGCATCAGATGTGAGGCCTGTAAGAAGGCTGGTAACCTGTACGACATCAGCAAGGACAACTCCCTG
  CAGGAACTGGACCAGCCGGCTGCCCCCGTGGCTGTGACATCCAACGCCTCCAGCACCAAGTACCCTCAAA
  GCCCGACTAATTCCAAGGCTCAGAAGAAGAATCGGAACAAACTGCGCCGGCAGCATTCCTACGACACCTT
  CGTGGACCTGCAGAAGGAGGAGGCCGCCTTGGCCCCACGCAGCGTGAGCCTGAAAGACAAGGGCCGATTC
  ATGGATGGGAGCCCCTACGCCCATATGTTTGAGATGCCAGCTGGTGAGAGCTCCTTTGCCAACAAGTCCT
  CAGTGCCCACTGCCGGACACCACCACAACAACCCCGGCAGCGGCTACATGCTCAGCAAGTCGCTCTACCC
  TGACCGGGTCACGCAAAACCCTTTCATCCCCACTTTTGGGGATGACCAGTGCTTGCTTCACGGCAGCAAA
  TCCTACTTCTTCAGGCAGCCCACGGTGGCAGGGGCGTCAAAAACAAGGCCGGACTTCCGGGCCCTTGTCA
  CCAATAAGCCAGTGGTGTCAGCCCTTCATGGGGCTGTGCCAGGTCGTTTCCAGAAGGACATTTGTATAGG
  GAACCAGTCCAACCCCTGTGTGCCTAACAACAAAAACCCCAGGGCTTTCAATGGCTCCAGCAATGGACAT
  GTTTATGAGAAACTTTCTAGTATTGAGTCTGATGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207651 representing NM_012574
  Red=Cloning site Green=Tags(s)
  
  MKPSAECCSPKFWLVLAVLAVSGSKARSQKSPPSIGIAVILVGTSDEVAIKDAHEKDDFHHLSVVPRVEL
  VAMNETDPKSIITRICDLMSDRKIQGVVFADDTDQEAIAQILDFISAQTLTPILGIHGGSSMIMADKDES
  SMFFQFGPSIEQQASVMLNIMEEYDWYIFSIVTTYFPGYQDFVNKIRSTIENSFVGWELEEVLLLDMSLD
  DGDSKIQNQLKKLQSPIILLYCTKEEATYIFEVANSVGLTGYGYTWIVPSLVAGDTDTVPSEFPTGLISV
  SYDEWDYGLPARVRDGIAIITTAASDMLSEHSFIPEPKSSCYNTHEKRIYQSNMLNRYLINVTFEGRNLS
  FSEDGYQMHPKLVIILLNKERKWERVGKWKDKSLQMKYYVWPRMCPETEEQEDDHLSIVTLEEAPFVIVE
  SVDPLSGTCMRNTVPCQKRIISENKTDEEPGYIKKCCKGFCIDILKKISKSVKFTYDLYLVTNGKHGKKI
  NGTWNGMIGEVVMKRAYMAVGSLTINEERSEVVDFSVPFIETGISVMVSRSNGTVSPSAFLEPFSADVWV
  MMFVMLLIVSAVAVFVFEYFSPVGYNRCLADGREPGGPSFTIGKAIWLLWGLVFNNSVPVQNPKGTTSKI
  MVSVWAFFAVIFLASYTANLAAFMIQEEYVDQVSGLSDKKFQRPNDFSPPFRFGTVPNGSTERNIRNNYA
  EMHAYMGKFNQRGVDDALLSLKTGKLDAFIYDAAVLNYMAGRDEGCKLVTIGSGKVFASTGYGIAIQKDS
  GWKRQVDLAILQLFGDGEMEELEALWLTGICHNEKNEVMSSQLDIDNMAGVFYMLGAAMALSLITFICEH
  LFYWQFRHCFMGVCSGKPGMVFSISRGIYSCIHGVAIEERQSVMNSPTATMNNTHSNILRLLRTAKNMAN
  LSGVNGSPQSALDFIRRESSVYDISEHRRSFTHSDCKSYNNPPCEENLFSDYISEVERTFGNLQLKDSNV
  YQDHYHHHHRPHSIGSTSSIDGLYDCDNPPFTTQPRSISKKPLDIGLPSSKHSQLSDLYGKFSFKSDRYS
  GHDDLIRSDVSDISTHTVTYGNIEGNAAKRRKQQYKDSLKKRPASAKSRREFDEIELAYRRRPPRSPDHK
  RYFRDKEGLRDFYLDQFRTKENSPHWEHVDLTDIYKERSDDFKRDSVSGGGPCTNRSHLKHGTGEKHGVV
  GGVPAPWEKNLTNVDWEDRSGGNFCRSCPSKLHNYSSTVAGQNSGRQACIRCEACKKAGNLYDISKDNSL
  QELDQPAAPVAVTSNASSTKYPQSPTNSKAQKKNRNKLRRQHSYDTFVDLQKEEAALAPRSVSLKDKGRF
  MDGSPYAHMFEMPAGESSFANKSSVPTAGHHHNNPGSGYMLSKSLYPDRVTQNPFIPTFGDDQCLLHGSK
  SYFFRQPTVAGASKTRPDFRALVTNKPVVSALHGAVPGRFQKDICIGNQSNPCVPNNKNPRAFNGSSNGH
  VYEKLSSIESDV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012574
• ORF Size: 4446 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012574.1, NP_036706.1
• RefSeq Size: 5259
• RefSeq ORF: 4449
• Locus ID: 24410
• MW: 166 kDa
• Gene Summary: subunit of the NMDA receptor glutamate gated ion channel; displays voltage dependent sensitivity to magnesium, calcium permeability, and glycine regulated activity; may facilitate learning and long term potentiation [RGD, Feb 2006]