Fen1 (NM_053430) Rat Tagged ORF Clone

CAT#: RR207787

Fen1 (Myc-DDK-tagged ORF) - Rat flap structure-specific endonuclease 1 (Fen1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fen1
• Synonyms: FEN-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207787 representing NM_053430
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAATTCAAGGCCTTGCCAAACTAATTGCTGATGTGGCCCCCAGTGCCATCCGTGAGAATGACATCA
  AGAGCTACTTTGGTCGCAAAGTGGCCATTGATGCCTCCATGAGCATTTACCAGTTCCTGATTGCTGTTCG
  TCAGGGTGGGGACGTACTGCAGAACGAGGAGGGGGAGACCACCAGCCACCTGATGGGCATGTTCTACCGT
  ACCATCCGCATGATGGAGAATGGCATCAAGCCGGTGTATATCTTTGATGGCAAACCACCACAGCTGAAGT
  CGGCCCAGCTGGCCAAACGCAGTGAGCGGCGTGCAGAGGCCGAGAAGCAGCTGCAGCAGGCTCAGGAGGC
  GGGGGCAGAGGAGGAGGTGGAAAAGTTCACCAAGCGGCTTGTGAAGGTCACCAAGCAACACAACGATGAG
  TGCAAACACCTGCTGAGCCTCATGGGCATCCCATACCTTGATGCGCCCAGCGAGGCAGAGGCCAGCTGTG
  CTGCCCTGGCAAAGGCTGGCAAAGTCTATGCCGCAGCCACGGAGGACATGGACTGCCTCACTTTTGGCAG
  CCCCGTGCTAATGCGACACTTAACTGCCAGTGAGGCCAAGAAGCTGCCCATCCAAGAGTTCCACCTGAGC
  CGCGTCCTGCAAGAGCTGGGTCTGAACCAGGAGCAGTTTGTGGATCTGTGCATCCTGCTGGGTAGCGACT
  ACTGTGAGAGTGTCCGTGGCATTGGACCCAAGCGGGCTGTGGACCTCATCCAGAAGCACAAGAGCATCGA
  GGAGATCGTGAGGCGGCTGGATCCCAGCAAGTACCCTGTTCCGGAAAACTGGCTCCACAAGGAAGCCCGG
  CAGCTCTTCCTGGAGCCGGAAGTAGTGGACCCAGAGTCTGTGGAGCTGAAGTGGAGTGAGCCAAACGAAG
  AAGAGTTGGTCAAATTTATGTGCGGTGAAAAGCAGTTTTCTGAAGAGCGGATTCGTAGTGGGGTCAAACG
  GCTGAATAAGAGCCGCCAAGGCAGCACCCAGGGACGCCTAGATGATTTCTTCAAGGTGACAGGCTCACTC
  TCCTCAGCCAAGCGCAAGGAGCCAGAGCCCAAGGGGCCTGCCAAGAAGAAAGCAAAGACTGGGGCAGCAG
  GGAAGTTCCGAAGGGGAAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207787 representing NM_053430
  Red=Cloning site Green=Tags(s)
  
  MGIQGLAKLIADVAPSAIRENDIKSYFGRKVAIDASMSIYQFLIAVRQGGDVLQNEEGETTSHLMGMFYR
  TIRMMENGIKPVYIFDGKPPQLKSAQLAKRSERRAEAEKQLQQAQEAGAEEEVEKFTKRLVKVTKQHNDE
  CKHLLSLMGIPYLDAPSEAEASCAALAKAGKVYAAATEDMDCLTFGSPVLMRHLTASEAKKLPIQEFHLS
  RVLQELGLNQEQFVDLCILLGSDYCESVRGIGPKRAVDLIQKHKSIEEIVRRLDPSKYPVPENWLHKEAR
  QLFLEPEVVDPESVELKWSEPNEEELVKFMCGEKQFSEERIRSGVKRLNKSRQGSTQGRLDDFFKVTGSL
  SSAKRKEPEPKGPAKKKAKTGAAGKFRRGK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053430
• ORF Size: 1140 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_053430.1, NP_445882.1
• RefSeq Size: 2192
• RefSeq ORF: 1143
• Locus ID: 84490
• MW: 42.6 kDa
• Gene Summary: removes RNA primers during Okazaki fragment maturation in DNA synthesis [RGD, Feb 2006]