Selplg (NM_001013230) Rat Tagged ORF Clone

CAT#: RR207935

Selplg (Myc-DDK-tagged ORF) - Rat selectin, platelet (p-selectin) ligand (Selplg), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Selplg
• Synonyms: Psgl1; Selpl
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207935 representing NM_001013230
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCCCACACTTCCTTCTGCTGCTGACCGTCTTGGGCCCTGGCAACAGCCTTCATCTGCAGGAGCCGT
  GGGGGCATGAAACCATGGAAGGCCTGGGCCCTGTGCATCTCCGGGAACGGAGACAGGTGGTTGGGGATGA
  TGATTTTGAATACCCCGACTACAGCGTGTATGGCACAGATCCCCCAGAAACGTTGATAAGTGTCCCCAAC
  ATGGTGGCGGCTCACCTTGAGCTTCGGACAATGGTGGCCGTGTTAGAGCAGAGAGTTTCCGCGGGGGCTG
  GAACTTCTGAGACTGTCACTGCGCAGGTTGCCACCACTGGCCCTGCTGACCCGGATACAGAAAGGCCAGC
  AGTTGGGATGCTGAGCACTGAATCTGTCACACAGCGGAGACTAAGCCCAGTGGAGATGACTACCCGGCTA
  GCACCCACGGAGGCAGAGACCTCACAGCCAGCACCCATAAAGGCAGAGACCTCACAGCCAGCACCCATAA
  AGGCAGAGACCTCACAGCCAGCACCCAGGGAGGCAGAGACCTCACAGCCAGCACCCACGGAGGCAGAGAC
  CTCACAGCCAGCACCCACAAAGGCAGAGACCTCACAGCCAGCACCCACAGAGGCAGAGACCTCACAGCCA
  GCACCCACGGAGGTAGAGACCTCACAGCCAGCACCCACAGAGGCAGAGACCTCACAGCCAGCACCCACGG
  AGGCAGAGACCTCACAGCCAGCGTCCACAGAGACAGAGACCACCCAGCTTCCCAGGAGTCAGGTTGTAGA
  AAGTCTATTTACAACATCTGCGGTCACAGAAGCCCCTTCTACGGAACCCACCGCCATGGAGACCCTGTCC
  ACAGAGGCTAATGAGTCTGCCATCTTCCTTGGGCCTTCCATTGGCCACTTACCTGACACCGGGCTGAAGA
  AAGGGCTGATTGTCACCCCTGGGAGTTCTCTGGCCCCAACCCCGCCAGGGACCTCGGACCTCATCCCGGT
  GAAGCAATGTTTGCTGGCCATTCTCATCTTAGCCTCCCTGGCCACCATCTTCCTTGTGTGCACGGTGGTG
  CTGGCCGTCCGCCTCTCCCGTAAGACGCACATGTACCCAGTGCGGAACTACTCCCCCACAGAGATGATCT
  GCATCTCATCCTTGCTTCCCGAGGGGGGTGACGGGGCCCCTGTCACAGCCAACGGGGGCCTGCCCAAGGT
  CCAGGACCAGAAGACAGAACGCAGTGGGGACCCGGATGGGGATGACCTCACCCTGCACAGCTTCCTCCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207935 representing NM_001013230
  Red=Cloning site Green=Tags(s)
  
  MFPHFLLLLTVLGPGNSLHLQEPWGHETMEGLGPVHLRERRQVVGDDDFEYPDYSVYGTDPPETLISVPN
  MVAAHLELRTMVAVLEQRVSAGAGTSETVTAQVATTGPADPDTERPAVGMLSTESVTQRRLSPVEMTTRL
  APTEAETSQPAPIKAETSQPAPIKAETSQPAPREAETSQPAPTEAETSQPAPTKAETSQPAPTEAETSQP
  APTEVETSQPAPTEAETSQPAPTEAETSQPASTETETTQLPRSQVVESLFTTSAVTEAPSTEPTAMETLS
  TEANESAIFLGPSIGHLPDTGLKKGLIVTPGSSLAPTPPGTSDLIPVKQCLLAILILASLATIFLVCTVV
  LAVRLSRKTHMYPVRNYSPTEMICISSLLPEGGDGAPVTANGGLPKVQDQKTERSGDPDGDDLTLHSFLP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001013230
• ORF Size: 1260 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001013230.1, NP_001013248.1
• RefSeq Size: 1263 bp
• RefSeq ORF: 1263 bp
• Locus ID: 363930
• Cytogenetics: 12q16
• MW: 44.3 kDa