Pde4a (NM_013101) Rat Tagged ORF Clone

CAT#: RR207991

Pde4a (Myc-DDK-tagged ORF) - Rat phosphodiesterase 4A, cAMP-specific (phosphodiesterase E2 dunce homolog, Drosophila) (Pde4a), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pde4a
• Synonyms: PHOSA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207991 representing NM_013101
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCTCCGGCCGCCCCCTCGGAAAGGAGCCTGTCTCTCTCTCTTCCGGGGCCCCGGGAGGGCCAGG
  CCACCCTGAAGCCGCCCCCCCAGCACCTGTGGCGGCAGCCGAGGACCCCGATCCGCATCCAGCAACGCGG
  CTACCCGGACAGTGCCGAGCGCTCAGAGACAGAGCGCTCACCGCACCGGCCCATAGAGCGCGCCGACGCC
  GTGGACACTGGCGACCGGCCAGGCCTGCGGACTACCCGCATGTCCTGGCCCTCGTCCTTCCACGGCACCG
  GTACCGGCGGAGGCAGCAGTAGGCGCTTGGAGGCAGAAAATGGGCCAACGCCATCCCCTGGCCGCAGCCC
  CCTGGACTCGCAGGCGAGCCCGGGGCTTGTGCTGCATGCTGGGGCCACCACCAGCCAGCGCCGCGAGTCC
  TTCCTCTACCGCTCAGACAGCGACTATGACATGTCACCGAAGGCTGTGTCCAGGAGCTCGTCTGTCGCCA
  GCGAAGCGCACGCTGAGGACCTCATTGTGACACCATTTGCCCAGGTGCTGGCCAGTCTCCGCAGCGTTCG
  AAGCAACTTCTCACTCTTAACCAATGTGCCCATCCCCAGCAACAAGAGGTCTCCACTGGGTGGCCCACCC
  TCTGTCTGCAAGGCCACACTGTCAGAGGAGACGTGCCAGCAGCTGGCCCGGGAGACCCTGGAAGAGCTGG
  ATTGGTGCCTGGAGCAGCTGGAGACCATGCAGACCTACCGCTCTGTCAGCGAGATGGCCTCACACAAGTT
  CAAAAGGATGCTGAACCGTGAGCTCACACACCTGTCGGAAATGAGCAGGTCAGGAAACCAGGTCTCAGAG
  TACATTTCCAACACATTCCTGGACAAGCAGAATGAAGTGGAGATCCCCTCACCCACACCTCGGCAGAGAG
  CCTTCCAGCAGCCCCCGCCGTCAGTGCTGCGACAGTCCCAGCCCATGTCTCAGATCACAGGGCTGAAAAA
  GCTGGTACACACTGGAAGCTTGAACACCAACGTCCCACGGTTTGGAGTCAAGACAGATCAAGAGGACCTC
  TTAGCACAAGAACTGGAGAACTTGAGCAAATGGGGCCTGAACATCTTTTGTGTGTCGGAGTACGCTGGAG
  GCCGCTCACTCAGCTGTATCATGTATACGATATTCCAGGAGCGGGACCTACTGAAGAAATTCCACATCCC
  TGTGGACACCATGATGATGTACATGCTGACCCTGGAGGACCACTACCATGCCGACGTGGCCTACCACAAC
  AGCCTGCACGCAGCGGATGTGCTGCAGTCCACACACGTGCTGCTGGCCACGCCCGCACTGGACGCTGTGT
  TCACAGACCTGGAGATTCTTGCTGCCCTCTTCGCTGCTGCCATCCACGATGTGGACCACCCTGGCGTCTC
  CAACCAGTTCCTAATCAACACCAATTCGGAGCTGGCGTTGATGTACAACGATGAGTCTGTGCTTGAGAAC
  CACCACCTGGCTGTGGGATTCAAGCTGCTGCAAGAAGAGAACTGCGACATCTTCCAGAACCTCAGCAAGC
  GCCAGCGGCAGAGCCTGCGCAAGATGGTCATCGACATGGTGCTGGCCACAGACATGTCCAAACACATGAC
  CCTCCTGGCTGACCTGAAGACTATGGTGGAGACGAAGAAAGTGACCAGCTCCGGAGTTCTCTTGCTGGAC
  AACTACTCTGACCGTATCCAGGTCCTCAGGAACATGGTGCACTGTGCAGACCTCAGCAATCCCACCAAGC
  CCCTGGAGCTGTACCGACAGTGGACCGACCGCATCATGGCTGAGTTCTTCCAGCAGGGCGACCGAGAACG
  GGAGCGTGGAATGGAGATCAGCCCCATGTGCGACAAGCACACAGCCTCTGTGGAGAAGTCTCAGGTGGGC
  TTCATCGACTACATTGTTCATCCATTGTGGGAGACATGGGCAGATCTCGTCCACCCGGATGCCCAAGACA
  TCCTGGACACGCTGGAAGACAACCGGGACTGGTACCACAGTGCCATTCGGCAGAGTCCTTCCCCACCCCT
  GGAAGAGGAGCCAGGGGGGCTTGGCCATCCGTCCCTGCCTGACAAGTTCCAATTTGAGCTCACCTTGGAG
  GAAGAGGAGGAAGAGGATTCCTTGGAGGTTCCAGGATTGCCTACCACTGAGGAAACCTTCCTGGCTGCAG
  AGGATGCCAGAGCTCAAGCTGTGGACTGGTCAAAGGTCAAAGGCCCGAGCACTACAGTGGTGGAAGTGGC
  AGAGCGCTTGAAGCAGGAGACCGCCTCAGCATATGGTGCTCCTCAGGAGTCCATGGAGGCTGTAGGCTGT
  TCCTTCAGCCCTGGGACCCCTATTCTGCCTGACGTGAGGACCCTATCCTCCTCAGAGGAGGCCCCGGGCC
  TCCTGGGCCTCCCCTCCACGGCGGCAGAGGTGGAGGCCCCAAGAGACCATCTGGCTGCCACGAGGGCTTG
  TTCTGCCTGCTCTGGGACCTCAGGAGACAATTCTGCCATCATCTCTGCTCCAGGCAGGTGGGGGTCAGGC
  GGAGACCCTGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207991 representing NM_013101
  Red=Cloning site Green=Tags(s)
  
  MEPPAAPSERSLSLSLPGPREGQATLKPPPQHLWRQPRTPIRIQQRGYPDSAERSETERSPHRPIERADA
  VDTGDRPGLRTTRMSWPSSFHGTGTGGGSSRRLEAENGPTPSPGRSPLDSQASPGLVLHAGATTSQRRES
  FLYRSDSDYDMSPKAVSRSSSVASEAHAEDLIVTPFAQVLASLRSVRSNFSLLTNVPIPSNKRSPLGGPP
  SVCKATLSEETCQQLARETLEELDWCLEQLETMQTYRSVSEMASHKFKRMLNRELTHLSEMSRSGNQVSE
  YISNTFLDKQNEVEIPSPTPRQRAFQQPPPSVLRQSQPMSQITGLKKLVHTGSLNTNVPRFGVKTDQEDL
  LAQELENLSKWGLNIFCVSEYAGGRSLSCIMYTIFQERDLLKKFHIPVDTMMMYMLTLEDHYHADVAYHN
  SLHAADVLQSTHVLLATPALDAVFTDLEILAALFAAAIHDVDHPGVSNQFLINTNSELALMYNDESVLEN
  HHLAVGFKLLQEENCDIFQNLSKRQRQSLRKMVIDMVLATDMSKHMTLLADLKTMVETKKVTSSGVLLLD
  NYSDRIQVLRNMVHCADLSNPTKPLELYRQWTDRIMAEFFQQGDRERERGMEISPMCDKHTASVEKSQVG
  FIDYIVHPLWETWADLVHPDAQDILDTLEDNRDWYHSAIRQSPSPPLEEEPGGLGHPSLPDKFQFELTLE
  EEEEEDSLEVPGLPTTEETFLAAEDARAQAVDWSKVKGPSTTVVEVAERLKQETASAYGAPQESMEAVGC
  SFSPGTPILPDVRTLSSSEEAPGLLGLPSTAAEVEAPRDHLAATRACSACSGTSGDNSAIISAPGRWGSG
  GDPA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013101
• ORF Size: 2532 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_013101.1, NM_013101.2, NM_013101.3, NP_037233.3
• RefSeq Size: 2914
• RefSeq ORF: 2535
• Locus ID: 25638
• MW: 93.4 kDa
• Gene Summary: human homolog catalyzes the hydrolysis of cAMP; activity is inhibited by rolipram [RGD, Feb 2006]