Tie1 (NM_053545) Rat Tagged ORF Clone

CAT#: RR208009

Tie1 (Myc-DDK-tagged ORF) - Rat tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (Tie1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tie1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR208009 representing NM_053545
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCTGGTGGGGATCCCCTTTCCTGCTCCCCATTCTTTTTCTGGCCTCTCATGTTGGTGCATCCGTGG
  ATTTAACACTGCTGGCCAATCTGCGTATCACCGAACCCCAGCGTTTCTTCCTGACCTGTGTGTCTGGTGA
  GGCGGGAGCAGGGAGGAGCTCCGACGTCTGGGGCCCGCCCCTGCTGCTGGAAAAGGATGATCGCATAGTG
  CGCACCTTCCCACCCGGGCAGCCCCTGCACCTCACTCGCAACGGTTCACACCAGGTCACGCTGCGGGGCT
  TCTCCAAGCCCTCGGATTTAGTAGGCGTCTTCTCCTGCGTGGGTGGAGCTGGCACGAGGCGCACTCGAGT
  CCTCTATGTGCACAACAGCCCGGGGGCACACCTGTTTCCAGACAAGGTCACACACACGGTGAACAAAGGT
  GATACCGCTGTGCTTTCTGCACGAGTGCACAAGGAAAAGCAGACTGATGTGATTTGGAAGAACAATGGAT
  CCTACTTCCATACCCTAGACTGGCATGAGGCCCATGATGGGCGGTTTCAACTGCAGCTCCAAAATGTACA
  GCCACCATCGAGTGGCATCTACAGCGCCACCTACCTAGAGGCCAGCCCCTTGGGCAGTGCCTTCTTTCGG
  CTCATCGTGCGAGGCTGTGAGGCTGGACGCTGGGGGCCGGGATGTGTCAAGGATTGCCCAGGCTGCCTGC
  ATGGAGGTGTCTGTCATGACCACGATGGCGAATGTGTGTGCCCCCCTGGATTCACTGGCACCCGCTGTGA
  GCAGGCCTGCAGAGAAGGTCGTTTTGGACAGAGCTGCCAGGAACAGTGCCCAGGCACAGCAGGCTGTCGG
  GGTCTCACCTTCTGCCTCCCAGATCCCTATGGCTGTTCTTGTGGATCTGGCTGGAGGGGAAGCCAGTGCC
  AGGAAGCATGTGCACCTGGTCATTTTGGGGCAGATTGTCGCCTCCAGTGTCAGTGTCAAAATGGTGGTAC
  TTGTGATCGGTTCAGCGGCTGCGTCTGCCCCTCCGGGTGGCATGGAGTCCACTGTGAAAAGTCAGACCGG
  ATCCCCCAGATCCTCAATGTGGCCACAGAGCTGGAGTTCAACTTAGGGACAATGCCCCGGATCAACTGTG
  CAGCTGCCGGGAATCCCTTCCCAGTACGGGGCAGCATGGAACTCCGCAAGCCAGACGGCACCATGCTCCT
  GTCTACCAAAGCCATTGTGGAGCCAGATAGGACCACAGCTGAGTTCGAGGTGCCCCGTTTGACTCTTGGG
  GACAGCGGGTTCTGGGAATGCCGTGTATCGACGTCTGGTGGCCAAGATAGCCGGCGCTTCAAGGTCAATG
  TCAAAGTACCCCCAGTGCCTCTGACTGCACCTCGGCTCCTGGCCAAGCAGAGCCGCCAGCTTGTGGTCTC
  CCCACTGGTCTCCTTCGGTGGGGATGGACCCATCTCCTCTGTCCGCCTGCACTACCGGCCCCAGGACAGC
  ATGATTACCTGGTCTGCCATTGTGGTGGATCCCAGTGAGAATGTGACATTAATGAACCTGAAGCCAAGGA
  CAGGATACAACGTTCGGGTGCAGCTGAGCCGGCCAGGGGAAGGAGGAGAAGGAGCCTGGGGTCCTTCGAC
  TCTCATGACTACGGACTGTCCTGAGCCTTTGCTGCAGCCGTGGGTAGAGAGCTGGAACGTGGAGGGTCCT
  GACAGGCTACGGGTGAGCTGGTCTCTACCCTCGGTGCCACTGTCTGGGGATGGTTTCCTGCTGCGTCTGT
  GGGATGGGGCCCGGGGACAGGAGAGGCGGGAGAACATCTCATCCCCCCAGGCCCGCACTGCCCTCCTGAC
  TGGACTCACGCCTGGCACGCACTACCAACTGGATGTGCGGCTGTATCACTGCACCCTCCTGGGCCCTGCC
  TCACCCTCTGCGCATGTGCACCTGCCCCTCAGCGGGCCTCCAGCTCCCCGCCACCTCCGTGCCCAGGCCC
  TCTCAGACTCTGAGATCCGTCTGATGTGGCAGCATCCTGAGGCTCCGCCTGGACCTATATCCAAGTACAT
  TGTGGAGATTCAGGTGGCCGGGGGCTCAGGAGACCCTCAGTGGATGGATGTAGACAAACCCGAGGAGACG
  AGCACCACCGTCCGTGGTCTCAATGCCAGCACACGCTACCTCTTCCGTGTGCGGGCCAGTGTGCAGGGTC
  TCGGCGACTGGAGCAACACAGTGGAAGAGACCACGCTGGGTAATGGGCTGCAGAGTGCGAGCCCAGTCCA
  AGAGAGCCGGGTGGCTGAAGACGGTCTGGATCAGCAGCTGGTCCTGGCTGTGGTAGGCTCCGTCTCTGCC
  ACCTGCCTCACCATCCTGGCTGCCCTTTTAGCCCTGGTGTGTATCCGAAGAAGCTGCCTACATCGGAGAC
  ATACCTTCACCTACCAGTCAGGATCGGGTGAAGAGACCATCCTGCAGTTCAGCTCAGGGACCTTGACATT
  GACCCGACGGCCAAAGCCACAGCCTGAGCCTTTGAGTTACCCTGTGCTGGAGTGGGAGGACATCACCTTT
  GAGGACCTCATCGGGGAGGGGAACTTCGGCCAAGTGATCCGGGCCATGATCAAGAAGGACGGACTCAAGA
  TGAACGCAGCCATCAAGATGCTGAAAGAGTATGCGTCTGAAAATGACCACCGAGACTTTGCAGGGGAACT
  AGAAGTTCTGTGCAAACTAGGACACCACCCCAATATTATCAACCTCTTGGGGGCCTGTGAGAACCGAGGC
  TACTTGTATATTGCGATCGAATATGCCCCGTATGGAAACCTGCTAGATTTCCTGAGAAAGAGCAGGGTCC
  TGGAGACTGATCCAGCTTTTGCTCGAGAGCATGGAACAGCCTCCACACTCAGCTCCCGGCAGCTGCTGCG
  CTTTGCCAGTGATGCAGCCAACGGGATGCAGTACCTTAGTGAGAAGCAGTTCATTCACAGGGACCTGGCT
  GCCCGAAATGTTCTGGTCGGAGAGAACCTGGCCTCCAAGATTGCAGACTTTGGCCTTTCTCGGGGGGAGG
  AAGTATATGTGAAGAAGACGATGGGCCGTCTTCCTGTGCGTTGGATGGCCATTGAGTCCCTCAACTACAG
  CGTCTACACTACCAAGAGTGACGTCTGGTCATTTGGGGTCCTCCTCTGGGAGATAGTGAGCCTTGGAGGC
  ACGCCCTACTGTGGCATGACCTGTGCTGAGCTCTACGAGAAACTGCCTCAAGGCTATCGCATGGAGCAGC
  CTCGAAACTGTGATGATGAAGTGTACGAGCTGATGCGGCAGTGCTGGCGGGACCGTCCCTATGAGCGCCC
  CCCTTTTGCCCAGATTGCACTACAGTTGGGCCGCATGCTGGAAGCCAGGAAGGCCTACGTGAACATGTCA
  CTGTTTGAGAACTTTACCTATGCAGGCATCGATGCCACAGCTGAGGAGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR208009 representing NM_053545
  Red=Cloning site Green=Tags(s)
  
  MVWWGSPFLLPILFLASHVGASVDLTLLANLRITEPQRFFLTCVSGEAGAGRSSDVWGPPLLLEKDDRIV
  RTFPPGQPLHLTRNGSHQVTLRGFSKPSDLVGVFSCVGGAGTRRTRVLYVHNSPGAHLFPDKVTHTVNKG
  DTAVLSARVHKEKQTDVIWKNNGSYFHTLDWHEAHDGRFQLQLQNVQPPSSGIYSATYLEASPLGSAFFR
  LIVRGCEAGRWGPGCVKDCPGCLHGGVCHDHDGECVCPPGFTGTRCEQACREGRFGQSCQEQCPGTAGCR
  GLTFCLPDPYGCSCGSGWRGSQCQEACAPGHFGADCRLQCQCQNGGTCDRFSGCVCPSGWHGVHCEKSDR
  IPQILNVATELEFNLGTMPRINCAAAGNPFPVRGSMELRKPDGTMLLSTKAIVEPDRTTAEFEVPRLTLG
  DSGFWECRVSTSGGQDSRRFKVNVKVPPVPLTAPRLLAKQSRQLVVSPLVSFGGDGPISSVRLHYRPQDS
  MITWSAIVVDPSENVTLMNLKPRTGYNVRVQLSRPGEGGEGAWGPSTLMTTDCPEPLLQPWVESWNVEGP
  DRLRVSWSLPSVPLSGDGFLLRLWDGARGQERRENISSPQARTALLTGLTPGTHYQLDVRLYHCTLLGPA
  SPSAHVHLPLSGPPAPRHLRAQALSDSEIRLMWQHPEAPPGPISKYIVEIQVAGGSGDPQWMDVDKPEET
  STTVRGLNASTRYLFRVRASVQGLGDWSNTVEETTLGNGLQSASPVQESRVAEDGLDQQLVLAVVGSVSA
  TCLTILAALLALVCIRRSCLHRRHTFTYQSGSGEETILQFSSGTLTLTRRPKPQPEPLSYPVLEWEDITF
  EDLIGEGNFGQVIRAMIKKDGLKMNAAIKMLKEYASENDHRDFAGELEVLCKLGHHPNIINLLGACENRG
  YLYIAIEYAPYGNLLDFLRKSRVLETDPAFAREHGTASTLSSRQLLRFASDAANGMQYLSEKQFIHRDLA
  ARNVLVGENLASKIADFGLSRGEEVYVKKTMGRLPVRWMAIESLNYSVYTTKSDVWSFGVLLWEIVSLGG
  TPYCGMTCAELYEKLPQGYRMEQPRNCDDEVYELMRQCWRDRPYERPPFAQIALQLGRMLEARKAYVNMS
  LFENFTYAGIDATAEEA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053545
• ORF Size: 3411 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_053545.1, NP_445997.1
• RefSeq Size: 3848
• RefSeq ORF: 3414
• Locus ID: 89806
• MW: 125.2 kDa
• Gene Summary: essential for vascular development and remodeling in the embryo; may mediate maintenance and repair of the adult vascular system [RGD, Feb 2006]