Ube3b (NM_001143894) Rat Tagged ORF Clone

CAT#: RR208033

Ube3b (Myc-DDK-tagged ORF) - Rat similar to ubiquitin protein ligase E3B (LOC687633), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ube3b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR208033 representing NM_001143894
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCACTGTATCTCAGACCTCCAGAGCATGGTTCCTCGACAAAGCCAGGCAGGCACGGGAAGAGAGGC
  TGGAGCAGAAGGAGCGGGAGCGCTCAGCAGTGCTGATCCAGGCACTAGTCCGCAGTTTCCTCTGTCGGAG
  GCGACTGCACAAAGATGTCAGGAGAGAGATCGATGAGTTTTTCACTGCTGATGAGTCTGGATCCAGTAAA
  AGAAGTGCACTTTGTATTTTTAAGATTGCCAGGAAGCTGCTGTTCATATGCAAAACTACCGAGGACAATG
  AGAGAATGGAGAAGCTGTGTCGTAGCATCCTGAGCAGCATGGATGCAGAGAATGAGCCAAAGGTATGGTA
  CGTGTCCCTGGCTCTTTCCAAGGACCTCACTCTGCTGTGGATTAAACAGATCAAGAGCATCCTGTGGTAC
  TGCTGTGAGCTTCTTGGGCAGCTCAAGCCAGAAATCTTACAGGACTCCAGGCTCATCACGCTGTACCTTA
  CCATGCTTGTCACCTTCACGGACACTTCAACATGGAAGATTCTCCGGGGGAAAGGTGAAAGCCTTCGGCC
  AGCCCTGAACCATATTTGTGCAAATATAATGGGCCACCTCAACCAGCGAGGATTCTACTCAGTGTTGCAG
  GTACTGCTGACCCGAGGCCTGGCCAGACCCCGGCCCTGTCTGTCCAAAAGCACGCTGACGGCTGCCTTCA
  CTCTGGCCCTGCGCCCTGTAGTTGCTGCGCAGTTCTCAGACAGTCTGATGCGACCGTTCATCATCCACAT
  CATGTCGGTACCTGCTCTCGTGGCTCATCTCAGCACCATGGCCCCTGAGCGTCTCGGGGTTTTAGAGTCC
  CATGACATGCTTCGTAAATTCATCGTCTTTCTGAGAGACAGAGACCGATGTCAAGATGCTTGCGAAAGTT
  TAGAAGGCTGCCATACCCTGTGCCTCATGGGCAATCTCCTGCACTTGGGCTCCCTCAACCTTAAGATGTT
  AGAGGAAGAAACAGATGGGTTTGTGAGCTTGCTCACCCAGATGCTGTGCTACTGTCAGAAGTATGTGTCC
  CAGAAGAAGTCCAACCTGACCCACTGGCACCCTGTGCTTGGCTGGTTTTCCCAGTCTGTGGATTACGGCC
  TCAATGACTCCATGTACCTGATCACCAAGCAGCTGCAGTTCCTGTGGGGCGTGCCTCTCATCCGCATTTT
  CTTCAGTGACATCCTCAGCAAGAAGCTGCTGGAGCCCCCAGAGCCAGTGCCTGCGCAGCCACAGCCATCC
  TCCCCACAGACCGTGCTTCCCGTGAAGAGTCTCCTCAAGCGTGCGTTTCAAAAGTCAGCTTCAGTTCGGA
  ATATCCTCAAGCCTGTCGGGGGCCGACGCGTGGACTCCACGGAGGTGCGGAAGGTCTGCAGCATCTGTGT
  GCTCTACCAGACCTCGCTGACAACCCTCACCCAGATCCGCTTGCAGATCCTCACAGGCCTTACCTACCTT
  GATGACCTGCTGCCCAAGCTCTGGGCTTTTATCTGTGAGCTGGGGCCCCATGGAGGGCTAAAGCTCTTCT
  TGGAGTGCCTGAACAATGACACTGGAGAGTCCAAGCAGCTCCTGGCCATGCTGATGCTCTTCTGTGACTG
  CTCCCGCCACCTCATCACCATCCTGGACGACATTGAAGTTTATGAGGAGCAGATCTCATTCAAGCTGGAA
  GAGCTGGTCACCATCTCTTCCTTCCTCAACTCCTTTGTGTTCAAGATGATCTGGGATGGAATCGTAGAGA
  ACGCCAAGGGTGAGACCTTGGAGCTGTTCCAGTCCGTCCATGGCTGGCTGATGGTGCTGTATGAACGAGA
  CTGCCGCAGGCGCTTCGCTCCAGAGGACCACTGGCTACGCAAGGATCTCAAGCCTGGCGTGCTCTTCCAG
  GAACTTGACAAGGACCGCAAGAGGGCTCAGCTGATCCTGCTGCACATTCCCCACGTCGTGCCTCACAAGA
  ACAGAGTTCTTCTGTTCCGGAACATGGTTATCAAGGAGAAAGAGAAGCTGGGGCTTGTGGAAACCAGCTC
  TGCCTCCCCTCACGTCACCCACATCACTATCCGAAGGTCCCGGATGCTGGAGGACGGCTATGAGCAACTG
  CGGCAGCTTCCTCAGCACGCCATGAAGGGCGCGATCCGAGTGAAGTTCGTCAGTGACCTTGGGGTGGACG
  AAGCTGGCATTGACCAGGACGGTGTCTTTAAGGAGTTTCTGGAAGAGATCATCAAGAGGGTGTTTGACCC
  AGCACTCAACCTGTTCAAGACAACCAGTGGGGATGAGAGGCTGTACCCGTCACCCACGTCCTACATCCAT
  GAGAACTACCTGCAGCTCTTTGAGTTTGTGGGGAAGATGCTGGGAAAGGCGGTGTATGAGGGGATCGTGG
  TGGACGTGCCCTTTGCCTCCTTCTTCCTGAGCCAAATGCTCGGGCACCACCACAGCGTCTTCTACAGCTC
  CGTGGATGAGCTGCCCTCCTTGGACTCGGAGTTCTATAAAAACCTCACCTCCATCAAGCGCTATGATGGA
  GACGTCGCAGACCTCGGGCTGACACTGTCTTACGATGAAGATGTCATGGGTCAGCTCGTCTGCCACGAAC
  TGGTTCCTGGAGGGAAGACCATCCCGGTCACAGATGAGAACAAAATTAGCTACATCCATCTGATGGCTCA
  TTTTCGAATGCACACTCAAATCAAGAACCAGACAGCTGCTCTCATTAGTGGCTTCCGTTCCATTATTAAG
  CCCGAGTGGATCCGAATGTTCTCTACCCCTGAGCTTCAACGTCTCATCTCCGGGGACAATGCCGAGATCG
  ATCTGGAAGACTTAAAGAAGCACACGGTGTACTACGGTGGCTTTCACGGGAGTCACAGAGTCATCGTCTG
  GCTCTGGGATATCCTGGCTTCTGACTTCACGCCTGGTGAAAGAGCCATGTTCCTGAAGTTTGTGACCAGC
  TGCTCCCGACCCCCGCTCCTAGGATTCGCCTACCTCAAACCTCCGTTCTCCATTCGCTGTGTGGAAGTGT
  CGGATGACCAGGACACTGGGGACACCCTGGGCAGCGTCCTCAGGGGCTTCTTCACCATCCGCAAGAGGGA
  GCCAGGCGGGCGTCTGCCCACCTCTTCCACTTGCTTCAACCTGCTTAAGCTGCCCAACTACAGCAAGAAA
  AGCGTACTCCGAGAGAAACTGCGCTACGCCATCAGCATGAACACAGGCTTTGAGCTCTCC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR208033 representing NM_001143894
  Red=Cloning site Green=Tags(s)
  
  MFTVSQTSRAWFLDKARQAREERLEQKERERSAVLIQALVRSFLCRRRLHKDVRREIDEFFTADESGSSK
  RSALCIFKIARKLLFICKTTEDNERMEKLCRSILSSMDAENEPKVWYVSLALSKDLTLLWIKQIKSILWY
  CCELLGQLKPEILQDSRLITLYLTMLVTFTDTSTWKILRGKGESLRPALNHICANIMGHLNQRGFYSVLQ
  VLLTRGLARPRPCLSKSTLTAAFTLALRPVVAAQFSDSLMRPFIIHIMSVPALVAHLSTMAPERLGVLES
  HDMLRKFIVFLRDRDRCQDACESLEGCHTLCLMGNLLHLGSLNLKMLEEETDGFVSLLTQMLCYCQKYVS
  QKKSNLTHWHPVLGWFSQSVDYGLNDSMYLITKQLQFLWGVPLIRIFFSDILSKKLLEPPEPVPAQPQPS
  SPQTVLPVKSLLKRAFQKSASVRNILKPVGGRRVDSTEVRKVCSICVLYQTSLTTLTQIRLQILTGLTYL
  DDLLPKLWAFICELGPHGGLKLFLECLNNDTGESKQLLAMLMLFCDCSRHLITILDDIEVYEEQISFKLE
  ELVTISSFLNSFVFKMIWDGIVENAKGETLELFQSVHGWLMVLYERDCRRRFAPEDHWLRKDLKPGVLFQ
  ELDKDRKRAQLILLHIPHVVPHKNRVLLFRNMVIKEKEKLGLVETSSASPHVTHITIRRSRMLEDGYEQL
  RQLPQHAMKGAIRVKFVSDLGVDEAGIDQDGVFKEFLEEIIKRVFDPALNLFKTTSGDERLYPSPTSYIH
  ENYLQLFEFVGKMLGKAVYEGIVVDVPFASFFLSQMLGHHHSVFYSSVDELPSLDSEFYKNLTSIKRYDG
  DVADLGLTLSYDEDVMGQLVCHELVPGGKTIPVTDENKISYIHLMAHFRMHTQIKNQTAALISGFRSIIK
  PEWIRMFSTPELQRLISGDNAEIDLEDLKKHTVYYGGFHGSHRVIVWLWDILASDFTPGERAMFLKFVTS
  CSRPPLLGFAYLKPPFSIRCVEVSDDQDTGDTLGSVLRGFFTIRKREPGGRLPTSSTCFNLLKLPNYSKK
  SVLREKLRYAISMNTGFELS
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001143894
• ORF Size: 3210 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001143894.1, NP_001137366.1
• RefSeq Size: 5201
• RefSeq ORF: 3213
• Locus ID: 687633
• MW: 122.6 kDa