Cd5l (NM_001025685) Rat Tagged ORF Clone

CAT#: RR208201

Cd5l (Myc-DDK-tagged ORF) - Rat Cd5 molecule-like (Cd5l), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cd5l
• Synonyms: MGC112716
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR208201 representing NM_001025685
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTATTGTTCAACTTGATACTGGCCCTCTTGACCATTTTTGTTGGACTGTGTTTCTCAGAGTCTC
  CAACTAGAGTGCGGCTAGTGGGAGGCGCCCACCGCTGTGAAGGGCGAGTGGAGGTGAAACACAACGGCCA
  GTGGGGGACTGTGTGCGATGACGGCTGGGACTTGATTGATGTGTCTGTGGTGTGCCGGGAGCTGAATTGT
  GGAGCAGCGAAAAAAACCCCGAGTGGTGCATCATATCATCCACCAGCATCAGAAGGGCAAAGCGTTCTTA
  TTCAAGGAGTTGAATGCAGTGGGGCGGAAGACATGCTGGCTCAATGTACGCTAAATTACGATGTTTTTGA
  TTGCTCGCATGCAGAAGATGCTGGGGTACAGTGTGAGAACCCAGACAATCCAGAGAATGTGCGGCTAGTA
  GAGGGCCTCGGGCGCTGCCAGGGTCGACTGGAGGTGTTCTACCAGGGCCAGTGGAGCACTGTGTGCAAAG
  CAGGCTGGAACTTACAGGCTTCAAAGGTGGTGTGCAGGCAGCTGGGCTGTGGGCGAGCACTGCTGGCCCA
  CAGATGCTGCAATAAGAATACCCAGGGCAAAGGACCCATCTGGATGAGCAAGATGTCATGTTCTGGACGA
  GAAGCAAACCTTCAGGACTGCCCTTTCAGTCCTTTGGAGAGCAATTGTACCCATGGCGAGGACACGTGGA
  TGGAATGCGAAGATCCCTTTGAGCTGAGGCTGGTAGGAGGAGACACCACCTGCTCTGGGAGGTTGGAGGT
  GCTGCACAAGGGTTCCTGGGGCTCTGTCTGTGATGACAGCTGGGGAGAAAAGGAGGACCAAGTGGTCTGC
  AAGCAACTGGGCTGTGGGAAGTCCCTCTTTCCATCCCCTAAAGCCCGCCAAAGCTTCGGGCCTGGGACAG
  GCCGCATCTGGCTGGATGACGTCAGTTGCTCAGGGAAGGAAGAGTCTCTTGAGCTCTGCCGGCACAGGCT
  GTGGGGATACCATGATTGCACGCACAAGGAAGATGTGGGCGTGATCTGCTCAGAGCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR208201 representing NM_001025685
  Red=Cloning site Green=Tags(s)
  
  MALLFNLILALLTIFVGLCFSESPTRVRLVGGAHRCEGRVEVKHNGQWGTVCDDGWDLIDVSVVCRELNC
  GAAKKTPSGASYHPPASEGQSVLIQGVECSGAEDMLAQCTLNYDVFDCSHAEDAGVQCENPDNPENVRLV
  EGLGRCQGRLEVFYQGQWSTVCKAGWNLQASKVVCRQLGCGRALLAHRCCNKNTQGKGPIWMSKMSCSGR
  EANLQDCPFSPLESNCTHGEDTWMECEDPFELRLVGGDTTCSGRLEVLHKGSWGSVCDDSWGEKEDQVVC
  KQLGCGKSLFPSPKARQSFGPGTGRIWLDDVSCSGKEESLELCRHRLWGYHDCTHKEDVGVICSEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001025685
• ORF Size: 1038 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025685.1, NP_001020856.1
• RefSeq Size: 1990 bp
• RefSeq ORF: 1041 bp
• Locus ID: 310693
• Cytogenetics: 2q34
• MW: 37.9 kDa