Cnot9 (NM_001009357) Rat Tagged ORF Clone

CAT#: RR208221

Rqcd1 (Myc-DDK-tagged ORF) - Rat rcd1 (required for cell differentiation) homolog 1 (S. pombe) (Rqcd1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cnot9
• Synonyms: Rqcd1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR208221 representing NM_001009357
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACAGCCTGGCAACGGCAGCGCCTGTGCCTACTGCACTGGCCCAGGTGGACAGAGAGAAGATCTATC
  AGTGGATCAATGAACTGTCCAGTCCTGAAACAAGGGAAAATGCTTTGCTGGAGCTGAGCAAGAAGCGAGA
  GTCTGTCCCTGACCTTGCGCCCATGCTGTGGCATTCATTCGGTACTATTGCAGCACTTTTACAGGAAATT
  GTAAATATTTATCCATCTATCAACCCTCCAACCTTGACAGCACACCAATCTAACAGGGTTTGCAATGCTC
  TAGCATTGCTACAGTGTGTGGCCTCACACCCAGAGACCAGGTCAGCCTTTCTTGCAGCACACATCCCACT
  CTTTTTGTACCCCTTTTTGCACACAGTCAGCAAAACTCGTCCCTTTGAATATCTTCGGCTCACCAGCCTT
  GGAGTTATTGGGGCCTTGGTTAAAACAGATGAGCAAGAAGTAATCAACTTTTTATTGACAACAGAAATCA
  TCCCTTTGTGTTTGCGCATCATGGAATCTGGAAGTGAACTGTCTAAAACGGTTGCCACATTCATCCTCCA
  GAAGATCCTCTTAGATGACACTGGTCTGGCTTATATATGTCAGACATATGAGCGTTTTTCCCATGTTGCC
  ATGATCTTGGGTAAAATGGTCCTGCAGCTATCCAAAGAACCATCTGCCCGTCTGCTGAAGCATGTAGTAA
  GATGTTACCTTCGACTCTCAGATAATCCCAGGGCACGTGAAGCACTCAGACAGTGCCTCCCTGACCAGCT
  GAAGGACACAACCTTTGCCCAGGTGCTAAAAGATGACACCACCACCAAACGCTGGCTTGCACAACTGGTG
  AAGAACCTGCAGGAGGGCCAAGTCACCGATCCCCGGGGAATTCCCCTGCCCCCTCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR208221 representing NM_001009357
  Red=Cloning site Green=Tags(s)
  
  MHSLATAAPVPTALAQVDREKIYQWINELSSPETRENALLELSKKRESVPDLAPMLWHSFGTIAALLQEI
  VNIYPSINPPTLTAHQSNRVCNALALLQCVASHPETRSAFLAAHIPLFLYPFLHTVSKTRPFEYLRLTSL
  GVIGALVKTDEQEVINFLLTTEIIPLCLRIMESGSELSKTVATFILQKILLDDTGLAYICQTYERFSHVA
  MILGKMVLQLSKEPSARLLKHVVRCYLRLSDNPRAREALRQCLPDQLKDTTFAQVLKDDTTTKRWLAQLV
  KNLQEGQVTDPRGIPLPPQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001009357
• ORF Size: 897 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001009357.1, NP_001009357.1
• RefSeq Size: 3399 bp
• RefSeq ORF: 900 bp
• Locus ID: 301513
• Cytogenetics: 9q33
• MW: 33.6 kDa