Naga (NM_001012120) Rat Tagged ORF Clone

CAT#: RR208245

Naga (Myc-DDK-tagged ORF) - Rat N-acetyl galactosaminidase, alpha (Naga), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Naga
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR208245 representing NM_001012120
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGCAGAAGACAGTGCTCTTACTGGCCCTGGTGGCTCAGGTACTGATGCTGGAGAATGGGCTACTGC
  GGACACCACCCATGGGCTGGCTGGCCTGGGAACGTTTCCGCTGTAACATCAACTGTGAGGAGGACCCGAA
  GAACTGCATCAGTGAGCGACTCTTTATGGAGATGGCTGACAGATTGGCTCAGGATGGATGGCGGGACCTA
  GGCTATGTATACCTCAACATCGATGACTGCTGGATTGGTGGACGTGATGCCACAGGCCGCCTGATACCTG
  ATCCCAAGCGATTCCCTCACGGGATTGCCTTCCTGGCTGACTATGCTCATTCTTTGGGTCTGAAGCTAGG
  CATCTATGAGGACATGGGCAAAATGACCTGCATGGGTTACCCTGGCACCACTCTAGACAAAGTGGAGCTA
  GACGCTGCAACCTTCGCAGAGTGGAAGGTGGACATGCTGAAACTGGATGGCTGTTACTCCACTCCCAAGG
  AGCGTGCAGAGGGGTACCCCAAGATGGCTGCTGCCCTGAATGCCACAGGCCGTCCCATCGCCTTTTCTTG
  CAGCTGGCCAGCCTATGAAGGGGGCCTACCCCCCAAGGTGAACTACACTGAGGTCGCCGGCACCTGCAAT
  CTCTGGCGCAACTATAAAGACATCCAGGACTCCTGGAAGAGTGTGCTGTCCATTCTGGACTGGTTTGTGA
  AACACCAGGACATACTGCAGCCCGTGTCGGGTCCTGGGCACTGGAATGACCCAGATATGCTGCTCATCGG
  GAACTTCGGCCTCAGCTTCGATGAATCCCGGGCCCAGATGGCCCTGTGGACAGTGCTGGCAGCCCCCCTC
  TTCATGTCCACGGACCTGCGTACCATCTCCCCCCAGAATATAGACATTCTTCAGAATCCACTCCTGATCA
  AAATCAATCAGGACCCCTTAGGCATCCAGGGACGCTTGATCTTCAAGAGCAAATCCCACATCGAAGTGTT
  CAAGCGGAATCTGTCTGACGATGCCAGTGCCCTGGTCTTCTTCAGCCGCAGGACAGACATGCCTTACCAC
  TTCCATTGTTCCCTCTTGGAACTGAACTACCCCAAAGGCAGTGTGTATGAGGGCCAGAACGTCTTCACAG
  GAGACATCATCAGCGGGCTCCACCCTGAAACCAACTTCACTGTGATCATCAACCCTTCCGGGGTTGTGAT
  GTGGTACCTATATCCCGTCAAGGGCTTGGGGATATACACAATGATGTCCCAGTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR208245 representing NM_001012120
  Red=Cloning site Green=Tags(s)
  
  MLQKTVLLLALVAQVLMLENGLLRTPPMGWLAWERFRCNINCEEDPKNCISERLFMEMADRLAQDGWRDL
  GYVYLNIDDCWIGGRDATGRLIPDPKRFPHGIAFLADYAHSLGLKLGIYEDMGKMTCMGYPGTTLDKVEL
  DAATFAEWKVDMLKLDGCYSTPKERAEGYPKMAAALNATGRPIAFSCSWPAYEGGLPPKVNYTEVAGTCN
  LWRNYKDIQDSWKSVLSILDWFVKHQDILQPVSGPGHWNDPDMLLIGNFGLSFDESRAQMALWTVLAAPL
  FMSTDLRTISPQNIDILQNPLLIKINQDPLGIQGRLIFKSKSHIEVFKRNLSDDASALVFFSRRTDMPYH
  FHCSLLELNYPKGSVYEGQNVFTGDIISGLHPETNFTVIINPSGVVMWYLYPVKGLGIYTMMSQL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012120
• ORF Size: 1245 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012120.1, NP_001012120.1
• RefSeq Size: 1785 bp
• RefSeq ORF: 1248 bp
• Locus ID: 315165
• Cytogenetics: 7q34
• MW: 46.9 kDa