Bckdha (NM_012782) Rat Tagged ORF Clone

CAT#: RR208247

Bckdha (Myc-DDK-tagged ORF) - Rat branched chain ketoacid dehydrogenase E1, alpha polypeptide (Bckdha), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Bckdha
• Synonyms: BCKDA; E1a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR208247 representing NM_012782
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGTAGCGATGTCCGCGGCCAAGATCTGGAGGCCGAGCCGTGGCCTGCGCCAGGCTGCCCTCTTGC
  TGTTGGGGCGACCTGGGGCTCGGGGCTTGGCTAGATTCCACCCCAGCAGGCAGCAGCAACAGCAGTTCCC
  ATCCCTGGATGACAAGCCCCAGTTCCCAGGGGCCTCTGCAGAGTTTGTAGACAAGCTTGAGTTCATCCAG
  CCCAATGTCATCTCCGGCATCCCCATCTACCGCGTCATGGACCGACAGGGCCAGATCATCAACCCCAGTG
  AAGATCCCCACCTGCCCCAGGAGGAGGTCCTGAAGTTGTACAGGAGCATGACGCTGCTCAACACCATGGA
  CCGCATTCTCTATGAGTCCCAGCGACAGGGCCGGATCTCCTTCTACATGACCAACTATGGCGAGGAGGGG
  ACACATGTGGGGAGTGCCGCTGCCCTGGAGCGAACGGACCTGGTGTTTGGCCAGTACCGGGAGGCAGGTG
  TGCTCATGTACCGGGACTACCCGCTGGAGCTGTTCATGGCCCAGTGCTACGGCAACGTGAGTGACCCAGG
  CAAGGGACGCCAGATGCCCGTTCACTACGGCTGCAAGGAGCGTCACTTCGTCACCATTTCCTCTCCACTG
  GCTACGCAGATCCCTCAGGCGGTAGGGGCAGCCTATGCTGCCAAGCGGGCCAATGCCAATCAGATCGTGA
  TCTGTTACTTTGGCGAGGGGGCAGCCAGTGAAGGGGATGCCCACGCCGGTTTCAATTTTGCTGCCACCTT
  GGAGTGTCCTATCATCTTCTTCTGCCGGAACAATGGCTATGCCATCTCCACACCAACCTCTGAGCAGTAC
  CGTGGGGATGGCATAGCGGCTCGGGGCCCTGGGTATGGCATCATGTCAATCCGTGTGGACGGCAACGATG
  TGTTTGCGGTGTACAATGCCACTAAGGAGGCCCGACGGCGGGCTGTGGCTGAGAACCAGCCCTTCCTCAT
  TGAGGCCATGACCTACAGGATCGGGCACCACAGCACCAGTGACGACAGCTCTGCGTACCGTTCGGTGGAT
  GAGGTCAATTACTGGGACAAGCAGGACCACCCGATCTCGAGACTGAGGCAGTACCTGCTGAACCAGGGTT
  GGTGGGATGAGGAACAGGAGAAGGCCTGGCGGAAGCAGTCACGAAAGAAGGTGATGGAGGCCTTTGAACA
  GGCTGAGCGGAAGCTAAAGCCCAACCCAAGCCTCCTCTTCTCCGATGTGTACCAGGAGATGCCTGCCCAG
  CTGCGCAGGCAGCAGGAGTCCCTGGCTCGGCACCTGCAGACCTACGGGGAGCACTACCCCCTGGACCACT
  TTGACAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR208247 representing NM_012782
  Red=Cloning site Green=Tags(s)
  
  MAVAMSAAKIWRPSRGLRQAALLLLGRPGARGLARFHPSRQQQQQFPSLDDKPQFPGASAEFVDKLEFIQ
  PNVISGIPIYRVMDRQGQIINPSEDPHLPQEEVLKLYRSMTLLNTMDRILYESQRQGRISFYMTNYGEEG
  THVGSAAALERTDLVFGQYREAGVLMYRDYPLELFMAQCYGNVSDPGKGRQMPVHYGCKERHFVTISSPL
  ATQIPQAVGAAYAAKRANANQIVICYFGEGAASEGDAHAGFNFAATLECPIIFFCRNNGYAISTPTSEQY
  RGDGIAARGPGYGIMSIRVDGNDVFAVYNATKEARRRAVAENQPFLIEAMTYRIGHHSTSDDSSAYRSVD
  EVNYWDKQDHPISRLRQYLLNQGWWDEEQEKAWRKQSRKKVMEAFEQAERKLKPNPSLLFSDVYQEMPAQ
  LRRQQESLARHLQTYGEHYPLDHFDK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012782
• ORF Size: 1338 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012782.1, NP_036914.1
• RefSeq Size: 1690
• RefSeq ORF: 1341
• Locus ID: 25244
• MW: 50.7 kDa
• Gene Summary: plays a role in the conversion of 3-methyl-2-oxobutanoate and lipoamide to S-(2-methylpropanoyl)dihydrolipoamide and CO2 [RGD, Feb 2006]